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1/43

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LAPORAN PRAKTIKUM : ISOLASI DNA

MAY 29, 2014 / ASTRIDSAFIRAIDHAM

LAPORAN PRAKTIKUM

GENETIKAPERCOBAAN II

ISOLASI DNA

NAMA : ASTRID SAFIRA IDHAM

NIM : H41113341

HARI/TANGGAL : KAMIS, 13 MARET 2014

KELOMPOK : VI (ENAM) B

ASISTEN : ANDRI NINDA

LABORATORIUM GENETIKA !URUSAN BIOLOGI

FAKULTAS MATEMATIKA DAN ILMU PENGETAHUAN ALAM

UNIVERSITAS HASANUDDIN

MAKASSAR

2014
https://astridsafiraidham.wordpress.com/https://astridsafiraidham.wordpress.com/2014/05/29/laporan-praktikum-isolasi-dna/#contenthttps://astridsafiraidham.wordpress.com/https://astridsafiraidham.wordpress.com/about/https://astridsafiraidham.wordpress.com/2014/05/29/laporan-praktikum-isolasi-dna/https://astridsafiraidham.wordpress.com/author/astridsafiraidham/https://astridsafiraidham.wordpress.com/2014/05/29/laporan-praktikum-isolasi-dna/#contenthttps://astridsafiraidham.wordpress.com/https://astridsafiraidham.wordpress.com/about/https://astridsafiraidham.wordpress.com/2014/05/29/laporan-praktikum-isolasi-dna/https://astridsafiraidham.wordpress.com/author/astridsafiraidham/https://astridsafiraidham.wordpress.com/


2/43

BAB I

PENDAHULUAN

I"1 L#$#% B&'##*

Deoxyribonucleic acidatau DNA meu!a"a# $e#%a&a "'m'a %a#(

!a)'#( !e#t'#( *a)am ma"+)u" +'*u!DNA meu!a"a# $e#%a&a %a#(

me#(a#*u#( '#-.ma$' (e#et'" ma"+)u" +'*u! *a' $atu (e#ea$' "e

(e#ea$' $e)a#ut#%a Ke$e)uu+a# DNA *a)am $uatu $e) a"a#

meme#tu" (e#.m e#.m me)'!ut' a('a# (e# %a#( -u#($'.#a)

mau!u# #.#-u#($'.#a) *a)am $e) .(a#'$me DNA (e#.m me)'!ut'

(e# *a#'#te(e# 3Su%., 2004

DNA a*a)a+ a$am #u")eat %a#( me#(a#*u#( mate' (e#et'" *a#

e-u#($' u#tu" me#(atu !e"ema#(a# '.).('$ $e)uu+ e#tu"

"e+'*u!a# $e5aa $e)u)e DNA te*a!at !a*a #u")eu$, m't'".#*'a,

*a# ")..!)a$ Pee*aa# "et'(a#%a a*a)a+ DNA #u")eu$ ee#tu"

)'#'e *a# ea$.$'a$' $a#(at eat *e#(a# !.te'# +'$t.#, $e*a#("a#

DNA m't.".#*'a *a# ")..!)a$ ee#tu" $'"u)a *a# t'*a"ea$.$'a$' *e#(a# !.te'# +'$t.# Se)a'# 'tu DNA m't.".#*'a *a#

")..!)a$ mem')'"' 5'' "+a$, %a'tu +a#%a me&a'$"a# $'-at$'-at %a#(

ea$a) *a' (a'$ 'u Se*a#("a# DNA #u")eu$ mem')'"' !.)a

!e&a'$a# $'-at *a' "e*ua .a#(tua D')'+at *a' .(a#'$me#%a,

$tu"tu DNA !."a'.t t'*a" mem')'"' !.te'# +'$t.# *a# ee#tu"

$'"u)a, $e*a#("a# DNA eu"a'.t ee#tu" )'#'e *a# mem')'"'

!.te'# +'$t.# 3K'$ma#, 2010

I$.)a$' DNA meu!a"a# )a#("a+ mem!e)aa' DNA Sa)a+ $atu

!'#$'!'$.)a$' DNA %a'tu *e#(a# $e#t'-u(a$' Se#t'-u(a$'

meu!a"a# te"#'" u#tu"mem'$a+"a# 5am!ua# e*a$a"a# eat


3/43

m.)e"u) ".m!.#e##%a M.)e"u) %a#( mem!u#%a' eat m.)e"u)

e$a a"a# ea*a *' a('a# a&a+ tau#( *a# m.)e"u) '#(a# a"a#

ea*a !a*a a('a# ata$ tau#( Ha$') $e#t'-u(a$' a"a#

me#u#u""a# *ua ma5am -a"$' %a#( te!'$a+, %a'tu $u!e#ata#

!a*a a('a# ata$ *a# !e)et !a*a a('a# a&a+ 3Ra5+mat, 2012

I"2 T++# P&%-.##

A*a!u# tuua# %a#( a"a# *'5a!a' !a*a !e5.aa# '$.)a$'

DNA '#' %a'tu u#tu" me#(eta+u' 5aa mem'$a+"a# 3me#('$.)a$'

DNA *a' 2 e#'$ ua+ %a#( *'a*'"a# $ea(a' $am!e), $eta u#tu"

me#(eta+u' "ea"t'-a# *ete(e# *a)am !.$e$ '$.)a$' DNA !a*a

$am!e) ua+

I"3 #$+ # T$ P&%-.##

Pe5.aa# '#' *')a"$a#a"a# !a*a +a' Kam'$, 16 Maet 2014,

!u"u) 1400 7 1860 ITA Pe5.aa# '#' etem!at *' :a.at.'um

B'.).(' Da$a, ;uu$a# B'.).(', Fa"u)ta$ Matemat'"a *a# I)mu

Pe#(eta+ua# A)am, U#'


4/43

BAB II

TIN!AUAN PUSTAKA

I$.)a$' DNA a*a)a+ !.$e$ !e#(e)uaa# DNA *a' tem!at#%a ea*a

3e"$ta"$' atau )'$'$ 'a$a#%a *')a"u"a# *e#(a# +.m.(e#a$' *a#

!e#ama+a# u=e e"$ta"$' atau u=e )'$'$ u#tu" me#5e(a+ DNA

u$a" 3Yu&.#., 200>

Pa*a $e) eu"a'.t'" tema$u" ta#ama# *a# +e&a# a('a# tee$a

*a' DNA ea*a !a*a #u")eu$ %a'tu .(a#e) %a#( *'!'$a+"a# *a'

$'t.!)a$ma *e#(a# mema# Nu")eu$ te*'' *a' 90 ? "e$e)uu+a#

DNA $e)u)e S'$a DNA a*a)a+ .(a#e) )a'# $e!et' m't.".#*'a *a#

")..!)a$ Kae#a DNA te*a!at !a*a #u")eu$, ma"a !e)u a*a#%a

met.*e !e)'$'$a# $e) $am!a' !ema#e#a# $e) D'ma#a met.*e

te$eut meu!a"a# a('a# *a' met.*e '$.)a$' DNA 3E).*, 2008

Pem'$a+a# DNA *a' mate' $e)u)e )a'##%a meu!a"a# +a) %a#($'(#'@"a# *a# me#(+au$"a# !e#%a))'#a# DNA me#a*' RNA *a#

ta#$)a$' RNA me#a*' !.te'# e)a#($u#( *a)am

".m!ateme#3 ua#( %a#( ee*a %a'tu $e5aa etuuttuut

*a)am #u5)eu$ *a# $'t.!)a$ma 3 E).*, 2008


5/43

Te*a!at .(a#e).(a#e) emema# (a#*a *' *a)am $'t.!)a$ma,

tema$u" m't.".#*'a a'" !a*a tumu+a# mau!u# +e&a# O)e+

"ae#a 'tu !e)u *')a"u"a# '$.)a$' DNA !a*a ta#ama# *a# +e&a#

u#tu" me#(eta+u' *a# mem!e)aa' DNA *a' ta#ama# *a# +e&a#

te$eut Se) eu"a'.t'" mem')'"' DNA )e'+ a#%a", )e#("a! *e#(a#

".m!.#e#".m!.#e# )a'# DNA ta#ama# *a# +e&a# te$'m!a#

*a)am #u5)eu$%a#( teu#("u$ .)e+ mema# I$.)a$' DNA

meu!a"a# )a#("a+ %a#( te!at u#tu" mem!e)aa' DNA P'#$'!#%a

a*a *ua, %a'tu $e#t'-u(a$' *a# !e$'!'ta$'Se#t'-u(a$' meu!a"a#

te"#'" u#tu" mem'$a+"a# 5am!ua# e*a$a"a# eat m.)e"u)

".m!.#e##%a M.)e"u) %a#( mem!u#%a' eat m.)e"u) e$a a"a#

ea*a *' a('a# a&a+ tau#( *a# m.)e"u) '#(a# a"a# ea*a

!a*a a('a# ata$ tau#( Ha$') $e#t'-u(a$' a"a# me#u#u""a# *ua

ma5am -a"$' %a#( te!'$a+, %a'tu $u!e#ata# !a*a a('a# ata$ *a#

!e)et !a*a a('a# a&a+ Pe$'!'ta$' meu!a"a# )a#("a+ %a#(

*')a"u"a# u#tu" me#(e#*a!"a# $uatu ".m!.#e# *a' 5am!ua#

3A)et, 1994

DNA !a*a ma"+)u" +'*u! *a!at *''$.)a$' $e5aa $e*e+a#a

Pe#('$.)a$'a# DNA $e5aa $e*e+a#a *a!at *')a"u"a# *e#(a#

meme5a+"a# *'#*'#( $e), mema# !)a$ma *a# mema# '#t' a'"

$e5aa me"a#'" mau!u# $e5aa "'m'a&' I$.)a$' DNA meu!a"a#

$uatu te"#'" %a#( *'(u#a"a# u#tu" mem!e.)e+ DNA mu#', %a'tu

ta#!a !.te'# *a# RNA *a' $uatu $e) *a)am a'#(a# Peme5a+a#*'#*'#( $e) $e5aa me"a#'" *a!at *')a"u"a# *e#(a# !em)e#*ea#

atau !e#((eu$ me#((u#a"a# m.ta *a# !'$t') Se*a#("a# $e5aa

"'m'a&' *a!at *')a"u"a# *e#(a# !eme'a# *ete(e# Pe#ama+a#


6/43

$au# 5a' *a# (aam *a!u a*a)a+ u#tu" me)'$'$"a# mema# '#t'

u#tu" me#(e)ua"a# '$' '#t' $e) %a#( e'$' DNA 3Ra5+mat, 2012

I$.)a$' DNA *a!at *')a"u"a# me)a)u' ta+a!a#ta+a!a# a#taa )a'#!e!aa$' e"$ta" $e), !emu#'a# DNA *a' e"$t$" $e) *a# !e$'!'ta$'

DNA Me$"'!u# '$.)a$' DNA *a!at *')a"u"a# *e#(a# ea(a' 5aa,

a"a# teta!' !a*a $et'a! e#'$ atau a('a# ta#ama# *a!at

meme'"a# +a$') %a#( ee*a, +a) '#' *'"ae#a"a# a*a#%a

$e#%a&a !.)'-e#.) *a# !.)'$a"a'*a *a)am ".#$e#ta$' t'#((' %a#(

*a!at me#(+amat !emu#'a# DNA ;'"a '$.)a$' DNA *')a"u"a#

*e#(a# $am!)e ua+, ma"a "a*a a' !a*a ma$'#(ma$'#( ua+

ee*a, *a!at meme' +a$') %a#( ee*ae*a !u)a Sema"'#

t'#((' "a*a a', ma"a $e) %a#( te)aut *' *a)am e"$ta" a"a#

$ema"'# $e*'"'t, $e+'#((a DNA %a#( te!et'$'!a$' u(a a"a# $e*'"'t

3K'$ma#, 2010

Pe#ama+a# *etee# *a)am '$.)a$' DNA *a!at me#%ea"a#

u$a"#%a mema#e $e), me)a)u' '"ata# %a#( *'e#tu" me)a)u' $'$'

+'*.-.'" *etee# *e#(a# !.te'# *a# )ema" !a*a mema#e

meme#tu" $e#%a&a )'!'* !.te'#*etee# ".m!)e"$ Se#%a&a

te$eut *a!at tee#tu" "ae#a !.te'# *a# )'!'* mem')'"' uu#(

+'*.@)'" *a# +'*.-.'", *em'"'a# u(a *e#(a# *etee#, $e+'#((a

*a!at meme#tu" $uatu '"ata# "'m'a 3K'$ma#, 2010

P'#$'! 7 !'#$'! *a)am me#('$.)a$' DNA 3T.+', 2012

1 me)'$'$ $e) $e5aa @$'", *e#(a# 5aa !e#((eu$a#

2 !eme5a+a# *'#*'#( $e)


7/43

6 !eme5a+a# mema# $e)

4 !em'$a+a# DNA *a' a+a# %a#( )a'#

Da)am '$.)a$' DNA, a+a# %a#( "'ta (u#a"a# 'a$a#%aeu!a a'#(a# tumu+a# atau a'#(a# +e&a#, u#tu" 'tu )a#("a+

!etama %a#( +au$ "'ta )a"u"a# a*a)a+ meme5a+"a# a'#(a#

me#a*' $e)$e) %a#( ma#*'' P.$e$ *')a"u"a# $e5aa me"a#'" atau

@$'" *e#(a# me#umu" atau me#((eu$ a+a# %a#( a"a# "'ta

(u#a"a# *e#(a# m.ta atau )e#*e Ke*ua a*a)a+ meme5a+"a#

*'#*'#( $e) *a# mema# $e) )a!'$a# !emu#("u$ DNA $tu"tu

utama !eme#tu" mema# *a# *'#*'#( $e) a*a)a+ )ema", u#tu" 'tu

"'ta (u#a"a# *etee# *a# (aam *a!u Ke*ua a+a# '#' *'(u#a"a#

u#tu" me)ua#(' *a# meu$a" $e) $e+'#((a '$' '#t' $e) 3DNA '$a

"e)ua 3T.+', 2012

Ta+a! $e)a#ut#%a a*a)a+ !em'$a+a# DNA *a' a+a# %a#( )a'#

Pem'$a+a# *')a"u"a# *e#(a# me#((u#a"a# et+a#.)/a)".+.) *'#('#

e".#$e#ta$' 909? Et+a#.)/A)".+.) t'*a" me)aut"a# DNA *a#

eat e#'$ a)".+.) %a#( )e'+ '#(a# *a' a' memuat DNA #a'" *a#

me)a%a#()a%a#( *' !emu"aa# 3T.+', 2012

CTAB atau Cetyl trimethylammonium bromide meu!a"a# $ee#'$

*etee# %a#( *a!at me#*e(a*a$' *'#*'#( $e), *e#atua$' !.te'#,

mem'$a+"a# "a.+'*at, meu$a" mema# $e) *a# me)aut"a#DNA A!a')a *'#*'#( $e) te*e(a*a$' ma"a $emua '$' $e) *a!at

"e)ua tema$u" DNA *a# *')e!a$"a# "e *a)am

u=e e"$ta"$'Da)am !.$e$ '$.)a$' DNA ta#ama#, !e#ama+a#

$e#%a&a !ee*u"$' $e!et' me5+a!t.eta#.) *a!at me#5e(a+


8/43

!.$e$ ."$'*a$' $e#%a&a -e#.)'" $e+'#((a me#(+amat a"t'


9/43

Met.*e '#' t'*a" memutu+"a# 'a%a %a#( )e'+ ma+a)

*'a#*'#("a# *e#(a# me#((u#a"a# "'t Se)a'# 'tu, "e)e'+a# *a'

e"ta"$' '#' a*a)a+ !'ta DNA %a#(*'!.)e+ )e'+ tea) ')a

*'a#*'#()a# *e#(a# e"ta"$' met.*e -e#.) *a# ta#!a -e#.) A"a#

teta!', *a' +a$') *e#(a# met.*e '#' ma$'+ te*a!at !'ta $mea *a#

DNA %a#( *'+a$')"a# )e'+ $e*'"'t *a'!a*a e"ta"$' *e#(a#

me#((u#a"a# "'t Ke#*a)a %a#( umum tea*' *a)am e"$ta"$' CTAB

a*a)a+ a*a#%a '#+''t. !a*a '#a#(, e#*a+#%a ".#$e#ta$'


10/43

METODE PERCOBAAN

III"1 A'#$ # B##

III"1"1 A'#$

A)at 7 a)at %a#( *'(u#a"a# *a)am !e5.aa# '#' a*a)a+ a)at

tu)'$ me#u)'$, tau#( ea"$', t'ma#(a#, (e)a$ aua, !'$au, ata#(

!e#(a*u", $!atu)a, )e#*e, !'!et tete$, *a# me$'#


11/43

T'(a e#'$ )auta# *ete(e# "emu*'a# *'ma$u""a# "e*a)am

ma$'#(ma$'#( tau#( ea"$' %a#( e'$' u$ ua+ $ea#%a" 4

m)

8 Tama+"a# 1 $!atu)a (aam *a!u "e*a)am ma$'#(ma$'#(

tau#( ea"$', "emu*'a# *'a*u" +'#((a )aut

> Cam!ua# "emu*'a# *'$a'#( $ea#%a" 2 "a)' *e#(a# "eta$

$a'#(

9 :a)u *'tama+"a# et+a#.) 9 ? $ea#%a" m) "e*a)am

ma$'#(ma$'#( 5am!ua#

10 :auta# "emu*'a# *'+.m.(e#"a# *e#(a# me#((u#a"a#

me$'#


12/43

DAFTAR PUSTAKA

A)et, B, 1994 Biologi olekuler Sel Edisi Kedua PT ame*'a

Pu$ta"a Utama ;;;;;;;a"ata

A$'$, 2010 De!nisi "solasi D#A dan anfaat "solasi

D#A+tt!//a$'$08Stu*e#t'!a5'* D'a"$e$ !a*a ta#((a) 18 Maet

2014, !a*a !u"u) 2600 ITA

E).*, S, 2008 $enetika Edisi Keempat E)a#((a ;a"ata

K'$ma#, 2010"solasi D#A Buah +tt!//"'$ma#>6&ee)5.m

D'a"$e$ !a*a ttttttttta#((a) 18 Maet 2014, !a*a !u"u) 2600 ITA

Ra5+mat, 2012 "solasi D#A% +tt!//a5+mat%u$u-).($!.t5.m

D'a"$e$ !a*a $$$$$$$ta#((a) 18 Maet 2014, !a*a !u"u) 2600

ITA
http://asris07/http://kirsman83.weebl/http://rachmatyusuf.blogspot.com/http://asris07/http://kirsman83.weebl/http://rachmatyusuf.blogspot.com/


13/43

Su%., 2004 $enetika Strata &% U#'


14/43

1"PEPAACarica papaya

(++, -#6%, %6)

(++, -#6%, %6)

2"T.#$Solanu

m lycopercium

(%6, ++, -#6%)

(++, -#6%, %6)

IV"1"2 T#&' P&*##$#

!ENISBUAH PERLAKUAN

HASIL PERCOBAAN

!UMLAHARNA AKTU

Pe!a%a

Caricapapaya

Dete(e#Buu"

Put'+ 7e#'#( Ce!at

Dete(e# Ca'Ku#'#( 7e#'#(

:amat

Dete(e# K'mKu#'#( 7!u5at Se*a#(

T.mat

Solanumlycopercium

Dete(e#Buu"

Put'+ 7e#'#( Ce!at

Dete(e# Ca' Be#'#( :amat

Dete(e# K'm Ku#'#( Se*a#(


15/43

IV"2 P#5#

P'#$'! utama *a)am '$.)a$' DNA a*a t'(a %a"#'

!e#(+a#5ua# 3)'$'$, e"ta"$' atau !em'$a+a# DNA *a' a+a#

!a*at $e!et' $e)u).$a *a# !.te'#, $eta

!emu#'a# DNA Se)a'# 'tu a*a 2 !'#$'! )a(' *a)am me)a"u"a#

'$.)a$' DNA, %a'tu $e#t'-u(a$' *a# !e$'!'ta$' P'#$'! utama

$e#t'-u(a$' a*a)a+ mem'$a+"a# $u$ta#$' e*a$a"a# eat e#'$

m.)e"u) *e#(a# 5aa meme'"a# (a%a $e#t'-u(a) $e+'#((a

$u$ta#$' %a#( )e'+ eat a"a# ea*a *' *a$a, $e*a#("a#

$u$ta#$' %a#( )e'+ '#(a# a"a# te)eta" *' ata$ Te"#'" $e#t'-u(a$'

te$eut *')a"u"a# *' *a)am $eua+ me$'# %a#( e#ama me$'#

$e#t'-u(a$' *e#(a# "e5e!ata# %a#( e


16/43

tee#tu" a#taa *ete(e# *e#(a# atat %a#( a*a !a*a ua+

Sete)a+ !e#ama+a# *ete(e# ta+a! $e)a#ut#%a %a'tu

!e#ama+a#a (aam *a!u $ea#%a" 1 $!atu)a "e ma$'#(ma$'#(

tau#( %a#( e'$' 5am!ua# ua+ *a# *ete(e#, tuua# *a'

!e#ama+a# (aam a*a)a+ u#tu" memu*a+"a# !em'$a+a# e#a#(

e#a#( DNA *a' 5am!ua# $e+'#((a e#a#(e#a#( te$eut a"a#

mu*a+ *'amat' Ha) '#' tea*' "ae#a Na *a)am (aam *a!at

meme#tu" '"ata# !a*a "utu #e(at'


17/43

mau!u# "a#*u#(a# a' *a' ma$'#(ma$'#( ua+ t'*a" $ama Da'

!e5.aa# t'*a" *'!e.)e+ DNA mu#' me)a'#"a# +a#%a DNA "a$a

atau e#a#( 7 e#a#( +a)u$ 3$u!e#ata#t *a)am um)a+ %a#(

$e*'"'t $eta e#*a!a# !ut'+ %a#( te)'+at Da' "e*ua ua+ DNA

"a$a %a#( !a)'#( te)'+at a*a !a*a ua+ !e!a%a, +a) 'tu tea*'

a"'at "a#*u#(a# a' !e!a%a )e'+ $e*'"'t *a' t.mat $e+'#((a

e"$ta" *a# DNA "a$a %a#( *'+a$')"a# .)e+ !e!a%a )e'+ a#%a"

*a'!a*a ua+ t.mat, $e)a'# 'tu e#*a!a# !ut'+ !a*a ua+ !e!a%a

)e'+ a#%a" *a'!a*a ua+ t.mat


18/43

BAB V

PENUTUP

V"1 K&56+'#

Da' !e5.aa# '$.)a$' DNA %a#( te)a+ *')a"u"a# *a!at

*'"eta+u' met.*e atau 5aa a(a'ma#a me#('$.)a$' DNA *a' ua+

%a#( *'a*'"a# $ea(a' $am!e) Ya'tu me)a)u 5aa !e#(+a#5ua#

3)'$'$, e"$ta"$', $eta !emu#'a# Pe#((u#aa# *ete(e# %a#(

ee*a *a)am !e5.aa# *')a"u"a# u#tu" me#(eta+u' *ete(e#

*a)am e#tu" a!a %a#( !a)'#( a"t'- *a)am me#(+a#5u"a#

mema# $e) !a*a $et'a! $am!e) ua+Sete)a+ *')a"u"a# !e5.aa#

te#%ata *ete(e# uu" me#(+a$')"a# DNA "a$a %a#( )e'+

te)'+at $eta e#*a!a# !ut'+ %a#( )e'+ a#%a"

V"2 S#%#

V"2"1 S#%# U$+ L#.%#$.%6+ Sea'"#%a )a.at.'um me#%e*'a"a# a)at %a#( 5u"u!

mema*a' $eta a)at %a#( 5u"u! a#%a", a(a !a"t'"um *a)am

ea)a# *e#(a# e@$'e#

V"2"2 S#%# U$+ A565$&

D'+aa!"a# a(a teu$ mem!e+at'"a# *a# mem'm'#(

!a"t'"a# $e)ama )a e)a#($u#(, a(a !a"t'"um ea)a# *e#(a#

a'"


19/43

Ade puji setyawati Biologi inspirasi

Jumat, 14 November 2014

!"#A! $NA %ANA&AN

ISOLASI DNA TANAMAN

A*e Pu' Set%a&at' 1

D+ B+'#tat' S Ha$ta', M B'.me*2

Fe$t% Au)'%au Ra+ma+, S S'2

Nu(.+. A*' Mau)a#a6

I#*+'#a Re'+a##'$+a6

1 Ma+a$'$&a ;uu$a# B'.).(', Fa"u)ta$ Sa'#$ *a# Te"#.).(', U#'


20/43

sa'i hi(au tersebut% )raktikum ini dilakukan dengan tu(uan agar praktikan mampumelakukan isolasi D#A dari tanaman Sa'i -i(au *Brassica sp%+% -asil setelah

penambahan kloroform dan isoamil alkohol *./0&+ &x 1olum sampel dandisentrifugasi terbentuk . fase yaitu fase a2oueous dan organik% Setelah

penambahan isopropanol dingin terlihat benang3benang tipis D#A%

Kata kunci 0 Tanaman, daun Sa'i hi(au, pemurnian D#A

I" D#5#% T&.%6


21/43

De.G%'. Nu5)e'5 a5'* 3DNA meu!a"a# $e#%a&a "'m'a %a#( !a)'#( !e#t'#( *a)amma"+)u" +'*u! DNA meu!a"a# $e#%a&a %a#( me#(a#*u#( '#-.ma$' (e#et'"ma"+)u" +'*u! *a' $atu (e#ea$' "e (e#ea$' $e)a#ut#%a DNA eu"a'.t t'*a" +a#%a*'um!a' !a*a #u")eu$, teta!' *a!at *'temu"a# !a*a m't.".#*'a *a# ")..!)a$DNA %a#( *''$.)a$' *a' ")..!)a$ me#u#u""a# $'-at ee#tu" $'"u)a, te*'' *a'

u#ta' (a#*a, e!)'"a$' $em'".#$e


22/43

De#(a# me#(eta+u' DNA ta#ama# $a&' ma"a *a!at me#(+a$')"a# !.te#$'%a#( 5u"u! e$a u#tu" me#(+a$')"a#


23/43

N. ama Ketea#(a#

1 Dau# +a$')!e#umu"a# *a#*'tama+"a#

u=e e"$ta"$'*a# *'


24/43

Su!e#ata# %a#(te)a+*'tama+"a#'$.!.!a#.) *'#('#1 G


25/43

te$eut $u)'t *'!'$a+"a# *a' e"$ta" a$am #u")eat Pemu"aa# $e) u#tu"me#(e)ua"a# a$am #u")eat#%a !a*a !a"t'"um '#' *e#(a# 5aa me"a#'" %a'tu*'(eu$ *e#(a# t'! $te') !a*a tau#( m'". atau *a!at u(a me#((u#a"a# a+a#"'m'a %a'tu #'t.(e# 5a' u#tu" me#*e(a*a$' *a# me)aut"a# ".m!.#e# *'#*'#($e)

Pa*a $aat !.$e$ *e$tu"$' a'#(a# ta#ama# *a!at me#%ea"a# *e(a*a$'!a*a DNA *e#(a# a*a#%a a"t'


26/43

A$am #u")eat a*a)a+ m.)e"u) +'*.@)'" *a# e$'-at )aut *a)am a' D'$am!'#('tu, !.te'# u(a me#(a#*u#( e$'*u +'*.-.'" %a#( me#(a"'at"a# !.te'# )aut*a)am !e)aut .(a#'" Be*a$a"a# $'-at '#', te*a!at eea!a met.*e*e!.te'#'$a$' e*a$a"a# !em')'+a# !e)aut .(a#'" B'a$a#%a !e)aut .(a#'"%a#( *'(u#a"a# a*a)a+ -e#.) atau ")..-.m %a#( me#(a#*u#( 4? '$.am') a)".+.)

Pe#((u#aa# ")..-.m '$.am') a)".+.) 3CIA e*a$a"a# !ee*aa# $'-at !e)aut.(a#'"

K)..-.m t'*a" *a!at e5am!u *e#(a# a' *a# "emam!ua##%a u#tu"me#*e!.te'#'$a$' e*a$a"a# "emam!ua# a#ta' !.)'!e!t'*a %a#( te*e#atua$'u#tu" ma$u" atau tem.')'$a$' "e *a)am -a$e a#taa ")..-.m 7 a' K.#$e#ta$'!.te'# %a#( t'#((' !a*a -a$e a#taa te$eut *a!at me#%ea"a# !.te'#me#(a)am' !e$'!'ta$' Se*a#("a# )'!'* *a# $e#%a&a .(a#'" )a'# a"a# te!'$a+!a*a )a!'$a# ")..-.m 3D.%)e, 1990

P.$e$ *e!.te'#'$a$' %a#( e-e"t'- e(a#tu#( !a*a e$a#%a -a$e a#taa

")..-.ma' P.$e$ '#' *a!at *')a"u"a# *e#(a# meme#tu" emu)$' *a' a' *a#")..-.m Ha) '#' +a#%a *a!at *')a"u"a# *e#(a# !e#((..(a# atau $e#t'-u(a$'%a#( "uat "ae#a ")..-.m t'*a" *a!at e5am!u *e#(a# a' I$.am') a)".+.)e-u#($' $ea(a' emu)$'@e *a!at *'tama+"a# "e ")..-.m u#tu" mema#tu!eme#tu"a# emu)$' *a# me#'#("at"a# )ua$ !emu"aa# ")..-.ma' %a#( ma#a!.te'# a"a# me#(a)am' !e$'!'ta$'

Pe#((u#aa# ")..-.m '$.am') a)".+.) '#' memu#("'#"a# u#tu" *'*a!at"a#DNA %a#( $a#(at mu#', #amu# *e#(a# u"ua# %a#( teata$ 32000070000 !DNA "emu*'a# *''"at *a' -a$e au.eu$ *e#(a# !e$'!'ta$' '$.!.!a#.) 3Mu)a*#.,2002 Sete)a+ !.$e$ e"$ta"$', DNA %a#( *'*a!at *a!at *'!e"at"a# me)a)u'

!e$'!'ta$' Pa*a umum#%a *'(u#a"a# '$.!.!a#.) *'#('# u#tu" me#(e#*a!"a#,me)e"at"a# *a# mem'$a+"a# DNA *a' )auta#

Pa*a $aat !e#ama+a# '$.!.!a#.) *'#('# te)'+at e#a#(e#a#( t'!'$ DNAta#ama# e&a#a !ut'+ %a#( me)a%a#( *' )auta# te$eut Ke*ua $e#%a&ate$eut a"a# mem!e$'!'ta$' DNA !a*a -a$e au.eu$ $e+'#((a DNA me#((um!a)meme#tu" $tu"tu @e *a# tee#tu" !e))et $ete)a+ *')a"u"a# $e#t'-u(a$'Me#uut He)*t 3200 a+&a !e$'!'ta$' e-u#($' u#tu" me#(+')a#("a# e$'*ue$'*u ")..-.m %a#( ea$a) *a' ta+a!a# e"$ta"$'

Me#uut Yu&.#., 3200> !'#$'!!'#$'! !e$'!'ta$' a#taa )a'# !etama,

me#uu#"a# "e)auta# a$am #u")eat *a)am a' Ha) '#' *'"ae#a"a# m.)e"u) a' %a#(!.)a me#(e)')'#(' m.)e"u) DNA *' )auta# au.eu$ Muata# *'!.)e !.$'t'- *a' a'e'#tea"$' *e#(a# muata# #e(at'- !a*a (u(u$ -.$-.*'e$te DNA I#tea"$' '#'me#'#("at"a# "e)auta# DNA *a)am a' I$.!.!a#.) *a!at e5am!u *e#(a# a',#amu# "ua#( !.)a *'a#*'#("a# a'

M.)e"u) '$.!.!a#.) t'*a" *a!at e'#tea"$' *e#(a# (u(u$ !.)a *a' a$am#u")eat $e+'#((a '$.!.!a#.) a*a)a+ !e)aut %a#( )ema+ a(' a$am #u")eatQ "e*ua,


27/43

!e#ama+a# '$.!.!a#.) a"a# me#(+')a#("a# m.)e"u) a' *a)am )auta# DNA$e+'#((a DNA a"a# te!e$'!'ta$'Q "et'(a, !e#((u#aa# '$.!.!a#.) *'#('# a"a#me#uu#"a# a"t'


28/43

DAFTAR PUSTAKA

*'a#a, D" 2009 Te"#'" '$.)a$' DNA (e#.m ta#ama# !a!a%a *a# eu" *e#(a#me#((u#a"a# m.*'@"a$' u=e CTAB% BuletinTeknik )ertanian 14(1): 121

.#ata 2008 Komunikasi pribadi% Ciri3ciri D#A murni dan penyebab keberhasilan sertakegagalan dalam )C4 dan elektroforesis%E)a#((a ;a"ata

.%)e ;; a#* D.%)e ;: 1990 I$.)at'.# .- !)a#t DNA -.m -e$+ t'$$ue F.5u$ M.$5.&1231161

ae), RE 2004 4#A ethodologies0 A 5aboratory $uide for "solation and Characteri6ation%Third Edition%E)$e


29/43

Lukisan Karya'amis, 20 &aret 2014

Laporan Genetika Isolasi DNA

LAPORAN PRAKTIKUM

GENETIKA

PERCOBAAN II

ISOLASI DNA

NAMA : NUR AFIAH SULAIMAN

NIM : H41113704

KELOMPOK : V (LIMA) B

HARI/TGL" PERCOBAAN : KAMIS/ 13 MARET 2014

ASISTEN : RISK NURHIKMAANI
http://catatantentangbiologi.blogspot.co.id/http://catatantentangbiologi.blogspot.co.id/


30/43

LABORATORIUM GENETIKA!URUSAN BIOLOGI

FAKULTAS MATEMATIKA DAN ILMU PENGETAHUAN ALAM

UNIVERSITAS HASANUDDIN

MAKASSAR

2014

BAB I

PENDAHULUAN

I"1 L#$#% B&'##*

DNA 3De.G%'.$e Nu5)e'5 A5'* a*a)a+ m.)e"u) utama %a#( me#(.*e $emua

'#-.ma$' %a#( *'utu+"a# u#tu" !.$e$ meta.)'$me *a)am $et'a! .(a#'$me DNA

te$u$u# ata$ 6 ".m!.#e# utama %a'tu (u)a *e."$''u$a, a$a #'t.(e# *a# -.$-at

%a#( te(au#( meme#tu" #u")e.t'*a M.)e"u) DNA '#' te'"at meme#tu"

".m.$.m, *a# *'temu"a# *' #u")eu$, m't.".#*'a *a# ")..!)a$ 3A(u$ *a#

Sa-aae#a#, 2014

DNA %a#( me#%u$u# ".m.$.m '#' meu!a"a# #u")e.t'*a a#("a! %a#(

teu$u$# +e)'G a#*a 3*.u)e +e)'G, %a#( a$a #'t.(e# *a# "e*ua e#a#(

!.)'#u")e.t'*a $a)'#( e!a$a#(a# *a)am !a$a#(a# %a#( teta! me)a)' '"ata#

+'*.(e# *a# a#taa #u")e.t'*a %a#( $atu *e#(a# %a#( )a'# *'+uu#("a# *e#(a#

'"ata# -.$-at DNA te*a!at *' *a)am $et'a! $e" ma"+)u" +'*u! *a# *'$eut $ea(a'

5eta" 'u "e+'*u!a# "ae#a m.)e"u) '#' e!ea# !e#t'#( $ea(a' !ema&a

'#-.ma$' +ee*'ta$ %a#( me#e#tu"a# $tu"tu !.te'# *a# !.$e$ meta.)'$me )a'#

3A(u$ *a# Sa-aae#a#, 2014

DNA *a!at me#(a)am' *e#atua$' *a# e#atua$' Se)a'# 'tu, DNA u(a '$a

*''$.)a$' I$.)a$' DNA a*a)a+ $uatu te"#'" *'ma#a +a$') a"+'#%a $ta#*$ta#* DNA


31/43

*a!at te!'$a+ *a)am e#tu" "um!u)a# $ta#* e&uu* e#a#(e#a#( !ut'+

Tuua# '$.)a$' DNA a*a)a+ me#*a!at"a# e"$ta" DNA !a*a a'#(a# atau $e) %a#(

*''#('#"a# 3A(u$ *a# Sa-aae#a#, 2014

I$.)a$' DNA '#' $a#(at !e#t'#( *a)am '*a#( '.te"#.).(' mau!u# '*a#(

'.).(' m.)e"u)e Pe#(e#a)a# '$.)a$' DNA $a#(at !e#t'#( me#('#(at '.te"#.).('

!a*a a"+'a"+' '#' $a#(at mau Te)e'+ u#tu" '*a#( '.).(' m.)e"u)e Beea!a

a"te' te)a+ e+a$') *''#t.*u"$' "e *a)am ta#ama# $e!et' !a*' "a!a$ *a# "e*e)a'

Pe#t'#(#%a '.te"#.).(' u#tu" !e"ema#(a# "ea#e"aa(ama# +a%at' *' ma$a

me#*ata#( meme)u"a# "eteam!')a# *a# !em'"'a# Sa)a+ $atu#%a a*a)a+ *e#(a#

me#(eta+u' 5aa !e#(um!u)a# DNA *a# !'#$'! *a$a#%a $ea(a' !'a"a# u#tu"

mem!e)aa' '.).(' m.)e"u)e !a*a "+u$u$#%a 3A(u$, *a# Sa-aae#a#, 2014

1"2 T++# P&%-.##

A*a!u# tuua# *a' !a"t'"um '#' a*a)a+

1 U#tu" me#(eta+u' 5aa atau met.*e %a#( e#a u#tu" mem'$a+"a# 3me#('$.)a$'

DNA *a' ua+ua+a#

2 Me#(eta+u' "ee-e"t'-a# *ete(e# *a# ua+ %a#( *'!a"a' u#tu" me)a"u"a#

!e5.aa# '$.)a$'

I"3 #$+ # T$

Pe5.aa# I$.)a$' DNA '#' *')a"$a#a"a# !a*a +a' Kam'$, 16 Maet 2014

!u"u) 14001800 ITA etem!at *' :a.at.'um B'.).(' Da$a, Fa"u)ta$

Matemat'"a *a# I)mu Pe#(eta+ua# A)am, U#'


32/43

BAB II

TIN!AUAN PUSTAKA

Stu*' me#(e#a' e"$'$te#$' a$am #u")eat !etama "a)' *')a"u"a# .)e+

F'e*'5+ M'e$5+e *a' ;ema# %a#( me#('$.)a$' '#t' *a' $e) *aa+ !ut'+ !a*a ta+u#

1>9 M'e$5+e me#emu"a# a+&a *' *a)am '#t' $e) te$eut te*a!at $e#%a&a

%a#( me#(a#*u#( -.$-at %a#( "emu*'a# *'#ama"a# #u")e'# Se)a#ut#%a !a*a

a"+' aa* "e19 te)a+ e+a$') *')a"u"a# !em'$a+a# a#taa DNA 3deoxyribonucleic

acid *a# RNA 3ribonucleic acid *a' !.te'#!.te'# %a#( me)e"at"a# m.)e"u) a$am

#u")eat te$eut !a*a $e) Pa*a a&a) ta+u# 1960a#, P :e


33/43

%a#( $ama *a# te(a" )uu$ te+a*a! a"$'$ u#ta'a# DNA U#ta'a# DNA mem!u#%a'

*ua )e"u"a# 3groo1e e"$te#a) %a'tu )e"u"a# e$a 3ma(or groo1e *a# )e"u"a# "e5')

3minor groo1e Ke*ua )e"u"a# te$eut mem!u#%a' !ea#a# $ea(a' tem!at

me)e"at#%a m.)e"u) !.te'# tete#tu 3Yu&.#., 200

U"ua# m.)e"u) DNA e


34/43

*e#(a# ua+ e"a*a a' e#*a Sema"'# t'#((' "a*a a' ma"a $e) %a#( te)aut *'

*a)am e"$ta" a"a# $ema"'# $e*'"'t, $e+'#((a DNA %a#( te!e$'!'ta$' u(a a"a#

$e*'"'t 3A(u$ *a# Sa-aae#a#, 2014

P.$e$ '$.)a$' DNA *'a&a)' *e#(a# !.$e$ e"$ta"$' DNA Ha) '#' etuua#

u#tu" mem'$a+"a# DNA *e#(a# !at'"e) )a'# %a#( t'*a" *''#('#"a# P.$e$ '#' +au$

*')a"u"a# *e#(a# +at'+at', $e+'#((a t'*a" me#%ea"a# "eu$a"a# !a*a *'#*'#(

$e), mema# $e) mema# !)a$ma *a# mema# '#t' a'" *e#(a# 5aa me"a#'"

mau!u# $e5aa "'m'a&' Caa me"a#'" '$a *')a"u"a# *e#(a# !em)e#*ea# atau

!e#((eu$ me#((u#a"a# m.ta *a# !'$t') Se*a#("a# $e5aa "'m'a&' *a!at

*e#(a# !eme'a# %a#( *a!at meu$a" mema# $e) *a# mema# '#t', $a)a+

$atu#%a a*a)a+ *ete(e# 3A(u$ *a# Sa-aae#a#, 2014

Se)a'# 'tu, $e5aa "'m'a )'$'$ *'#*'#( $e) *a!at *')a"u"a#

*e#(a# me#ama+"a# $e#%a&a "'m'a $e!et' )'$.'m, EDTA 3et+')e#*'am'#

tetaa$etat, *a# SDS 3$.*'um

*.*e$') $u)-at Da)am +a) '#' -u#($' EDTA a*a)a+ $ea(a' !eu$a" $e)

*e#(a# 5aame#('"at ma(#e$'um I.# '#' e-u#($' u#tu" mem!eta+a#"a# '#te('t

a$ $e)

mau!u#mem!eta+a#"a# a"t'


35/43

$'$' +'*.-.'" *ete(e# *e#(a# !.te'# *a# )ema" !a*a mema# meme#tu"

$e#%a&a )'!'* !.te'#*ete(e# ".m!)e"$ Se#%a&a te$eut *a!at tee#tu"

"ae#a !.te'# *a# )'!'* mem')'"' uu#( +'*.@)'" *a# +'*.-.'", *em'"'a# u(a

*e#(a# *ete(e# $e+'#((a *a!at meme#tu" $uatu '"ata# "'m'a Kemu*'a#!e#ama+a# (aam 3NaC) e-u#($' u#tu" me)aut"a# DNA *a# $ete)a+ 'tu

*'e'"a# et+a#.) *'#('# 9? u#tu" me#(um!u)"a#/me#((um!a)"a# DNA 3A(u$

*a# Sa-aae#a#, 2014

E)e"t.-.e$'$ DNA meu!a"a# te"#'" u#tu" mem'$a+"a# $am!e) DNA

e*a$a"a# ata$ u"ua# 3eat m.)e"u) *a# $tu"tu @$'" m.)e"u)#%a e) %a#(

'a$a *'(u#a"a# a#taa )a'# a(a.$a E)e"t.-.e$'$ (e) a(a.$a *a!at *')a"u"a#

u#tu" mem'$a+"a# $am!e) DNA *e#(a# u"ua# *a' eea!a atu$ +'#((a 20000

!a$a#( a$a 3! 3D&'*.$e!ut., 199>

M.)e"u) DNA emuata# #e(at'- $e+'#((a *' *a)am me*a# )'$t'" a"a#

em'(a$' me)a)u' mat'"$ (e) me#uu "utu !.$'t'- 3a#.*e Ma"'# e$a u"ua#

m.)e"u)#%a, ma"'# e#*a+ )au m'(a$'#%a Beat m.)e"u) $uatu -a(me# DNA *a!at

*'!e"'a"a# *e#(a# mema#*'#("a# )au m'(a$'#%a *e#(a# )au m'(a$' -a(me#

-a(me# m.)e"u) DNA $ta#*a 3DNA ma"e %a#( te)a+ *'"eta+u' u"ua##%a

V'$u)'$a$' DNA $e)a#ut#%a *')a"u"a# *' a&a+ !a!aa# $'#a u)ta


36/43

METODE PERCOBAAN

III"1 A'#$

A)at %a#( *'(u#a"a# *a)am !e5.aa# '#' a*a)a+ !'$au, me$'# )e#*e,

t'ma#(a#, (e)a$ aua, !e#(a*u", !e#%a'#( 3t'$$u / "a!a$ / "eta$ $a'#(, $!atu)a,

tau#( ea"$', !'!et tete$, *a# me$'#


37/43

8 Me#(amat' !.$e$ t'mu)#%a DNA, me)'!ut' &a"tu %a#( *'!e)u"a#, &a#a, $eta

a#%a" $e*'"'t#%a DNA %a#( tee#tu"

W

BAB IV

HASIL DAN PEMBAHASAN


38/43

IV"1 H#56' P&%-.##

IV11 ama +a$') Pe5.aa#

;e#'$ ua+ See)um Se$u*a+

T.mat Dete(e#

Buu"

Dete(e#

5a'

Dete(e#

"'m

Pe!a%a Dete(e#

Buu"

Dete(e#

5a'


39/43

Dete(e#

"'m

IV"1"2 T#&' P&*##$#

N. ;e#'$ Bua+ Pe)a"ua# Ha$') Pe#(amata# ;um)a+

a#a a"tu

1 T.mat

Solanumlycopersiu

m

Dete(e#

Buu"

Be#'#(, a*a

e#*a!a#

:amat

Dete(e#

Ca'

Be#'#( Se*a#(

Dete(e#

K'm

Put'+ Be#'#( :e'+

Ce!at

2 Pe!a%a

Carica papaya

Dete(e#

Buu"

Put'+

Ke"u#'#("u#'#(a#

:e'+

Ce!at

Dete(e#

Ca'

Put'+ "eu+ :amat

Dete(e#

K'm

Put'+ $u$u Se*a#(

IV"2 P#5#

Pa"t'"um '$.)a$' DNA '#' *')a"u"a# etuua# u#tu" me#(eta+u' !e#(au+

ma5am ua+ *a# e#'$ *etee# te+a*a! "ua)'ta$ DNA %a#( *'+a$')"a# *a)am

!.$e$ '$.)a$' Se+'#((a *a)am !a"t'"um '#' *'uat


40/43

*ete(e# *a# e#'$ ua+ a(a *a!at me#(eta+u' ua+ *a# e#'$ *ete(e# ma#a

%a#( me#(+a$')"a# DNA !a)'#( a(u$ *a# a'" ;e#'$ ua+ %a#( *'(u#a"a# a*a)a+

t.mat Solanum lycopersicum$ea(a' 5.#t.+ ua+ %a#( me#(a#*u#( a#%a" a'

*a# !e!a%a Carica papaya$ea(a' ua+ %a#( mem')'"' "a#*u#(a# a' t'*a" te)a)ua#%a" Se*a#("a# u#tu" *ete(e# %a#( *'!a"a' a*a)a+ *ete(e# uu" me" Su-,

*ete(e# 5a' me" R'#$., *a# *ete(e# "'m me" BuCeam

Pa*a *a$a#%a !'#$'! '$.)a$' DNA a*a t'(a %a'tu !e)'$'$a# $e), e"$ta"$' *a#

!emu#'a# Pa*a !a"t'"um "a)' '#' %a#( !etamatama *')a"u"a# 'a)a+

me#(+a#5u"a# $am!e) ua+ *e#(a# 5aa me"a#'" atau !em)e#*ea# ua+ u#tu"

me)'$'$/ meu$a" *'#*'#( $e)% Seme#taa 'tu *'uat !u)a )auta# *ete(e# *e#(a#

5aa me#5am!u"a# "et'(a e#'$ *ete(e# 3Su-, R'#$. *a# Bu5eam "e *a)am

aua*e$ Pe#(a*u"a# *')a"u"a# *e#(a# +at'+at' a(a t'*a" tea*' a#%a" u$a

%a#( *a!at me#(+a)a#(' !e#(amata#

Kemu*'a# u$ ua+ *'tama+"a# *e#(a# )auta# *ete(e# Pe#ama+a#

)auta# *ete(e# e-u#($' u#tu" me)'$'$ *'#*'#( $e) tumu+a# %a#( te*a!at *a)am

)auta# $am!e) I#' *'$ea"a# "ae#a $'-at *a' *ete(e# $ama *e#(a# $'-at *'#*'#(

$e) %a#( +'*.-.'", $e+'#((a tea*' '"ata# *'a#taa "e*ua#%a *a# me#%ea"a#

*'#*'#( $e) u$a"

:a)u *'tama+"a# (aam *a!u *a# *'a*u" Pe#('$.)a$'a# DNA me#((u#a"a#

(aam *a!u *e#(a# tuua# u#tu" meme"at"a# DNA Ha) '#' *a!at tea*' "ae#a

'.# Na%a#( *'"a#*u#( .)e+ (aam mam!u meme#tu" '"ata# *e#(a# "utu

#e(at'


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teua' te$eut e*a$a"a# eat m.)e"u) Et+a#.) e!ea# *a)am !e#(um!u)a#

*a# !e#((um!a)a# DNA "ae#a $'-at#%a %a#( *'#('#

Kemu*'a# $ete)a+ *'amat' !.$e$ t'mu)#%a DNA, me)'!ut' &a"tu %a#(

*'!e)u"a#, &a#a, $eta a#%a" $e*'"'t#%a DNA %a#( tee#tu" Ma"a +a$') %a#(

*'*a!at"a# %a'tu )auta# %a#( !a)'#( 5e!at meme#tu" (um!a)a# DNA a*a)a+

t.matBu"'m *a# t.matSu-

Ha) '#' et.)a" e)a"a#( *e#(a# te.' %a#( me#%ata"a# a+&a ua+ !e!a%a

a"a# )e'+ a#%a" me#(+a$')"a# (um!a)a# DNA "ae#a mem')'"' "a#*u#(a# a'

%a#( )e'+ $e*'"'t *a'!a*a t.mat $e+'#((a DNA %a#( te!e$'!'ta$' )e'+ a#%a"

!a*a ua+ !e!a%a

Ke#%ataa# '#' u(a t'*a" $ea)a# *e#(a# a#((a!a# *ete(e# %a#( !a)'#( a'"

*'(u#a"a# u#tu" '$.)a$' DNA a*a)a+ *ete(e# uu", "emu*'a# *ete(e# 5a', )a)u

*ete(e# "'m Da' +a$') te$eut ma"a %a#( *a!at *'$'m!u)"a# a+&a t.mat *a#

BuCeam !a)'#( a#%a" me#(+a$')"a# DNA Da# *a' "et'(a e#'$ *etee# %a#(

*'(u#a"a#, %a#( !a)'#( $e*'"'t me#(+a$')"a# DNA a*a)a+ *etee# R'#$. :auta#

*e#(a# *etee# uu" Su- *a# Bu "'m me#(+a$')"a# DNA *e#(a# um)a+ %a#(

5u"u! a#%a" Namu# "et'(a e#'$ *etee# te$eut t'*a" *a!at *'a#*'#("a#

ma#a %a#( )e'+ a'" *a)am me#('$.)a$' DNA "ae#a !a*a $et'a! !e)a"ua#

me#u#u""a# +a$') %a#( e)at'- $ama

Kemu#("'#a# te*a!at "e$a)a+a# !a*a $aat *')a"u"a# !em)e#*ea# ua+

!e!a%a %a#( te)a)u )ama *a# te)a)u +a)u$ $e+'#((a DNA %a#( *'*a)am#%a

te5a'"5a'" +a#5u *a# me#%ea"a# "e(a(a)a# !a*a $aat !a*a '$.)a$' DNA

Se)a'# 'tu !a*a $aat !.$e$ !e#%a'#(a# *a# !e#ama+a# et+a#.) tea*' "e+a'$a#

a+a# *a# "ae#a#%a te*a!at $ea('a# !e5.aa# %a#( e)um tu#ta$ *a#

me#((u#a"a# a+a# !e#((a#t' %a#( teata$ "a!a$'ta$#%a *'a#*'#("a# a+a#

%a#( $eme$t'#%a *'(u#a"a# *a)am !a"t'"um


42/43

BAB V

PENUTUP

V"1 K&56+'#

A*a!u# "e$'m!u)a# *a' !a"t'"um '#' a*a)a+

1 U#tu" me)a"u"a# '$.)a$' DNA met.*e atau te"#'" %a#( *'(u#a"a# !a*a *a$a#%a

a*a t'(a %a'tu !e)'$'$a# $e), e"$ta"$', *a# !emu#'a# Pe)'$'$a# *a!at *')a"u"a#

*e#(a# 5aa me"a#'" mau!u# "'m'a, $a)a+ $atu#%a *e#(a# 5aa *')e#*e *a#

*'5am!u )auta# *ete(e# E"$ta"$' *a!at *')a"u"a# *e#(a# !e#ama+a# (aam

*a!u 3NaC) *a# !emu#'a# $a)a+ $atu met.*e#%a a*a)a+ *e#(a# meme'"a#

)auta# et+a#.) *'#('# 9?

2 ;e#'$ *ete(e# *a# ua+ %a#( *'!a"a' u#tu" me)a"u"a# '$.)a$' DNA $a#(at

e!e#(au+ !a*a DNA %a#( *'+a$')"a#

V"2 S#%#

A*a!u# $aa# u#tu" !a"t'"um '#' $ea'"#%a a)at *a# a+a# )a.at.'um

*'tama+ u#tu" me#5e(a+ tea*'#%a "e"ua#(a# a+a# %a#( *a!at

me#(a"'at"a# "ea$a)a+a# *a# "ua#( a"uat#%a +a$') %a#( *'*a!at"a# !a*a

!a"t'"um '#'


43/43

DAFTAR PUSTAKA

A(u$, R.$a#a *a# Sa-aa#a'# 2014 )enuntun )raktikum $enetika%Ma"a$$a

D&'*.$e!ut. 199> Dasar3Dasar ikrobiologi Damata# ;a"ata

K'ma)), ; ;.+# 1992Biologi E)a#((a ;a"ata

Mu)a*#. 2002 Seputar Teknologi 4ekayasa $enetika Pu$ta"a 'au$a+a Mu*a *a#

USESE F.u#*at'.# B.(.

Su+a$.#. *a# '*%a$tut', Utut 200 )elatihan Singkat Teknik Dasar )engklonan $en

IPB Pe$$ B.(.

Yu&.#., T'&'.&. 200 Biologi olekular E)a#((a ;a"ata

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