86

Lampiran 1. Data Hasil Pengamatan Perubahan Fluktuasi Berat Badan

Mencit (Mus musculus L) Selama Perlakuan

Perlakuan Minggu

ke- Ulangan Perubahan BB Total Rata2

K- 1 U-1 2 8 2

1 U-2 3

1 U-3 1

1 U-4 2

2 U-1 2 7 2

2 U-2 1

2 U-3 2

2 U-4 2

3 U-1 2 6 2

3 U-2 2

3 U-3 1

3 U-4 1

4 U-1 -1 2 1

4 U-2 2

4 U-3 0

4 U-4 1

5 U-1 -1 -4 -1

5 U-2 -1

5 U-3 -1

5 U-4 -1

6 U-1 -1 -6 -2

6 U-2 -1

6 U-3 -2

6 U-4 -2

7 U-1 0 8 2

7 U-2 3

7 U-3 3

7 U-4 2

8 U-1 3 5 1

8 U-2 0

8 U-3 0

8 U-4 2

9 U-1 2 6 2

9 U-2 2

9 U-3 1

9 U-4 1

10 U-1 -1 -4 -1

10 U-2 -4

10 U-3 0

10 U-4 1

87

K+ 1 U-1 1 5 1

1 U-2 2

1 U-3 1

1 U-4 1

2 U-1 3 5 1

2 U-2 1

2 U-3 -1

2 U-4 2

3 U-1 0 2 1

3 U-2 1

3 U-3 0

3 U-4 1

4 U-1 2 7 2

4 U-2 2

4 U-3 0

4 U-4 3

5 U-1 4 6 2

5 U-2 2

5 U-3 3

5 U-4 -3

6 U-1 -1 -1 0

6 U-2 1

6 U-3 -1

6 U-4 0

7 U-1 -3 -6 -2

7 U-2 -3

7 U-3 1

7 U-4 -1

8 U-1 2 8 2

8 U-2 3

8 U-3 1

8 U-4 2

9 U-1 1 0 0

9 U-2 0

9 U-3 -

9 U-4 -1

10 U-1 -1 -1 0

10 U-2 0

10 U-3 -

10 U-4 0

P1 1 U-1 1 6 2

1 U-2 2

1 U-3 2

1 U-4 1

88

2 U-1 -1 6 2

2 U-2 3

2 U-3 3

2 U-4 1

3 U-1 1 5 1

3 U-2 1

3 U-3 2

3 U-4 1

4 U-1 0 2 1

4 U-2 2

4 U-3 0

4 U-4 0

5 U-1 1 -2 -1

5 U-2 0

5 U-3 -1

5 U-4 -2

6 U-1 -1 -2 -1

6 U-2 0

6 U-3 0

6 U-4 -1

7 U-1 0 -13 -3

7 U-2 -4

7 U-3 -4

7 U-4 -5

8 U-1 2 16 4

8 U-2 3

8 U-3 5

8 U-4 6

9 U-1 0 -1 0

9 U-2 1

9 U-3 0

9 U-4 -2

10 U-1 0 1 0

10 U-2 2

10 U-3 -1

10 U-4 -

P2 1 U-1 2 10 3

1 U-2 3

1 U-3 4

1 U-4 1

2 U-1 3 8 2

2 U-2 -1

2 U-3 1

2 U-4 5

89

3 U-1 3 12 3

3 U-2 6

3 U-3 1

3 U-4 2

4 U-1 1 5 1

4 U-2 -5

4 U-3 7

4 U-4 2

5 U-1 -1 2 1

5 U-2 0

5 U-3 1

5 U-4 2

6 U-1 1 1 0

6 U-2 0

6 U-3 1

6 U-4 -1

7 U-1 -3 -3 -1

7 U-2 1

7 U-3 -1

7 U-4 0

8 U-1 2 4 1

8 U-2 0

8 U-3 1

8 U-4 1

9 U-1 0 -2 -1

9 U-2 -1

9 U-3 0

9 U-4 -1

10 U-1 -1 -2 -1

10 U-2 -1

10 U-3 -1

10 U-4 1

P3 1 U-1 -1 6 2

1 U-2 2

1 U-3 2

1 U-4 3

2 U-1 -3 5 1

2 U-2 0

2 U-3 4

2 U-4 4

3 U-1 1 4 1

3 U-2 0

3 U-3 2

3 U-4 1

90

4 U-1 0 2 1

4 U-2 4

4 U-3 -2

4 U-4 0

5 U-1 -1 -1 0

5 U-2 -3

5 U-3 2

5 U-4 1

6 U-1 4 5 1

6 U-2 1

6 U-3 0

6 U-4 0

7 U-1 -2 -4 -1

7 U-2 -2

7 U-3 0

7 U-4 0

8 U-1 -2 -2 -1

8 U-2 0

8 U-3 0

8 U-4 0

9 U-1 1 2 1

9 U-2 1

9 U-3 0

9 U-4 0

10 U-1 -2 -6 -2

10 U-2 -1

10 U-3 -3

10 U-4 0

91

Lampiran 2. Data Hasil Pengamatan Histologi Kelenjar Mammae Mencit

(Mus musculus)

1. Rata-rata Diameter Lumen Duktus Mammae Mencit

Perlakuan Ulangan Ke-

Total Rata-rata 1 2 3 4

K- 29,38 28,46 25,54 25,14 108,52 27,13

K+ 15,24 16,46 - 19,05 50,75 16,92

P1 20,60 25,29 19,67 - 65,56 21,85

P2 23,39 24,86 25,81 24,82 98,88 24,72

P3 28,42 24,06 25,47 28,47 106,42 26,61

2. Rata-rata Tebal Epitel Duktus Mammae Mencit

Perlakuan Ulangan Ke-

Total Rata-rata 1 2 3 4

K- 9,30 9,42 10,82 9,71 39,25 9,81

K+ 13,86 17,04 - 14,49 45,39 15,13

P1 11,77 14,06 13,80 - 39,63 13,21

P2 12,27 10,42 9,47 9,88 42,04 10,51

P3 9,95 7,82 10,35 10,24 38,36 9,59

3. Rata-rata Lumen Alveoli Mammae Mencit

Perlakuan Ulangan Ke-

Total Rata-rata 1 2 3 4

K- 26,87 26,25 28,66 27,14 108,92 27,23

K+ 15,33 16,43 - 16,55 48,31 16,10

P1 19,52 18,03 17,98 - 55,53 18,51

P2 24,81 26,30 25,62 24,11 100,84 25,21

P3 25,41 26,68 27,29 27,34 106,72 26,68

92

4. Rata-rata Tebal Epitel Alveoli Mammae Mencit

Perlakuan Ulangan Ke-

Total Rata-rata 1 2 3 4

K- 9,10 11,60 7,14 8,03 35,87 8,97

K+ 15,89 13,64 - 16,55 46,08 15,36

P1 9,30 13,01 15,40 - 37,71 12,57

P2 11,47 10,26 9,55 12,38 43,66 10,92

P3 9,72 9,16 9,77 8,36 37,01 9,25

93

Lampiran 3. Ciri Fisik Mencit Selama Perlakuan

Minggu

ke- Perlakuan

Perlakuan

K- K+ P1 P2 P3 P4

1

1 N N N N N N

2 N N N N N N

3 N N N N N N

4 N N N N N N

2

1 N N N N N N

2 N N N N N N

3 N N N N N N

4 N N N N N N

3

1 N N N N N N

2 N N N N N N

3 N N N N N N

4 N N N N N N

4

1 N N N N N N

2 N N N N N N

3 N N N N N N

4 N N N N N N

5

1 N N N N N N

2 N N N N N N

3 N N N N N N

4 N N Ps N N N

6

1 N Apcv Apcv N Apcv Apcv

2 N Apcv Apcv

+Apcd Apcv Apcv Apcd

3 N Apcv Apcv

+Apcd Apcv Apcv Apcv

4 N Apcv Apcv

+Ps Apcv Apcv Apcv

7

1 N Apcv Apcv Apcv Apcv Apcv

2 N Apcv Apcv Apcv Apcv Apcd

3 N Apcv

+Asc Apcv Apcv Apcv Apcv

4 N Apcv Apcv Apcv Apcv Apcv

8

1 N Apn Apn Apn Apn Apn

2 N Apn Apn Apn Apn Apn

3 N M Apn Apn Apn Apn

4 N Apn Apcv

+Ps Apn Apn Apn

9

1 N Apn Apn Apn Apn Apn

2 N Apn Apn Apn Apn Apn

3 N M Apn Apn Apn Apn

4 N Apn M Apn Apn Apn

10

1 N Apn Apn Apn Apn M

2 N Apn Apn Apn Apn M

3 N M Apn Apn Apn M

4 N Apn M Apn Apn M

Keterangan: N: Normal, Asc: Ascites (Perut buncit), Alopecia (Kerontokan bulu); Apcv (Alopecia

cephalo ventral): kerontokan bulu bagian ventral kepala, Apcd (Alopecia cephalo

dorsal): kerontokan bulu bagian dorsal kepala, Apn (Alopecia normal): membaik, Ps:

Paralisis (Lumpuh), M: mati.

94

Lampiran 4. Perhitungan SPSS ANOVA dan Uji Duncan

1. Pengaruh Ekstrak Daun Sirsak (Annona muricata L.) dan DMBA Terhadap

Fluktuasi Berat Badan Mencit (Mus musculus)

a. Uji Normalitas

One-Sample Kolmogorov-Smirnov Test

Perlakuan FluktuasiBB Minggu

N 160 157 160

Normal Parametersa Mean 2.51 .57 5.50

Std. Deviation 1.122 2.017 2.881

Most Extreme Differences Absolute .176 .135 .107

Positive .168 .135 .107

Negative -.176 -.128 -.107

Kolmogorov-Smirnov Z 2.231 1.688 1.356

Asymp. Sig. (2-tailed) .000 .007 .051

a. Test distribution is Normal.

b. Uji Homogenitas

Test of Homogeneity of Variances

FluktuasiBB

Levene Statistic df1 df2 Sig.

.338 3 153 .798

c. Uji Anova

ANOVA

FluktuasiBB

Sum of Squares df Mean Square F Sig.

Between Groups 8.235 3 2.745 .671 .571

Within Groups 626.313 153 4.094

Total 634.548 156

95

2. Pengaruh Ekstrak Daun Sirsak (Annona muricata L.) dan DMBA Terhadap

Tebal Epitel Duktus Mammae Mencit (Mus musculus)

a. Uji Normalitas

One-Sample Kolmogorov-Smirnov Test

Perlak Rata2

N 14 14

Normal Parametersa Mean 2.64 11.8157

Std. Deviation 1.151 2.52803

Most Extreme Differences Absolute .193 .210

Positive .140 .210

Negative -.193 -.141

Kolmogorov-Smirnov Z .723 .784

Asymp. Sig. (2-tailed) .672 .570

a. Test distribution is Normal.

b. Uji Homogenitas

Test of Homogeneity of Variances

Rata2

Levene Statistic df1 df2 Sig.

.294 3 10 .829

c. Uji Anova

ANOVA

Rata2

Sum of Squares df Mean Square F Sig.

Between Groups 65.420 3 21.807 12.347 .001

Within Groups 17.662 10 1.766

Total 83.082 13

96

d. Uji Duncan

Rata2

Duncan

Perlak N

Subset for alpha = 0.01

1 2 3

P3 4 9.5900

P2 4 10.5100 10.5100

P1 3 13.2100 13.2100

K+ 3 15.1300

Sig. .386 .024 .088

Means for groups in homogeneous subsets are displayed.

97

3. Pengaruh Ekstrak Daun Sirsak (Annona muricata L.) dan DMBA Terhadap

Diameter Lumen Duktus Mammae Mencit (Mus musculus)

a. Uji Normalitas

One-Sample Kolmogorov-Smirnov Test

Perlak Rata2

N 14 14

Normal Parametersa Mean 2.64 22.9721

Std. Deviation 1.151 4.13257

Most Extreme Differences Absolute .193 .183

Positive .140 .103

Negative -.193 -.183

Kolmogorov-Smirnov Z .723 .685

Asymp. Sig. (2-tailed) .672 .736

a. Test distribution is Normal.

b. Uji Homogenitas

Test of Homogeneity of Variances

Rata2

Levene Statistic df1 df2 Sig.

2.804 3 10 .094

c. Uji Anova

ANOVA

Rata2

Sum of Squares df Mean Square F Sig.

Between Groups 178.772 3 59.591 13.780 .001

Within Groups 43.244 10 4.324

Total 222.016 13

98

d. Uji Duncan

Rata2

Duncan

Perlak N

Subset for alpha = 0.01

1 2

K+ 3 16.9167

P1 3 21.8533 21.8533

P2 4 24.7200

P3 4 26.6050

Sig. .011 .017

Means for groups in homogeneous subsets are

displayed.

99

4. Pengaruh Ekstrak Daun Sirsak (Annona muricata L.) dan DMBA Terhadap

Tebal Epitel Alveoli Mammae Mencit (Mus musculus)

a. Uji Normalitas

One-Sample Kolmogorov-Smirnov Test

Perlak Rata2

N 14 14

Normal Parametersa Mean 2.64 11.7471

Std. Deviation 1.151 2.75180

Most Extreme Differences Absolute .193 .206

Positive .140 .206

Negative -.193 -.122

Kolmogorov-Smirnov Z .723 .769

Asymp. Sig. (2-tailed) .672 .595

a. Test distribution is Normal.

b. Uji Homogenitas

Test of Homogeneity of Variances

Rata2

Levene Statistic df1 df2 Sig.

2.625 3 10 .108

c. Uji Anova

ANOVA

Rata2

Sum of Squares df Mean Square F Sig.

Between Groups 68.852 3 22.951 7.757 .006

Within Groups 29.589 10 2.959

Total 98.441 13

100

d. Uji Duncan

Rata2

Duncan

Perlak N

Subset for alpha = 0.01

1 2

P3 4 9.2525

P2 4 10.9150

P1 3 12.5700 12.5700

K+ 3 15.3600

Sig. .036 .060

Means for groups in homogeneous subsets are

displayed.

101

5. Pengaruh Ekstrak Daun Sirsak (Annona muricata L.) dan DMBA Terhadap

Diameter Lumen Alveoli Mammae Mencit (Mus musculus)

a. Uji Normalitas

One-Sample Kolmogorov-Smirnov Test

Perlak Rata2

N 14 14

Normal Parametersa Mean 2.64 22.2429

Std. Deviation 1.151 4.61132

Most Extreme Differences Absolute .193 .229

Positive .140 .177

Negative -.193 -.229

Kolmogorov-Smirnov Z .723 .856

Asymp. Sig. (2-tailed) .672 .457

a. Test distribution is Normal.

b. Uji Homogenitas

Test of Homogeneity of Variances

Rata2

Levene Statistic df1 df2 Sig.

.206 3 10 .890

c. Uji Anova

ANOVA

Rata2

Sum of Squares df Mean Square F Sig.

Between Groups 268.853 3 89.618 118.190 .000

Within Groups 7.582 10 .758

Total 276.435 13

102

d. Uji Duncan

Rata2

Duncan

Perlak N

Subset for alpha = 0.01

1 2 3

K+ 3 16.1033

P1 3 18.5100

P2 4 25.2100

P3 4 26.6800

Sig. 1.000 1.000 .052

Means for groups in homogeneous subsets are displayed.

103

Lampiran 5. Peta Konsep Penelitian

Aktivator P53

Sel Kanker

Acetogenins

Produksi ATP turun

difusi

NADH dehidrogenase

Mitokondria

ATP

ATP yang berada di dinding mitokondria

Sel kanker kekurangan Energi

Sel Kanker Mati

Sel normal

Mengaktifkan p53

104

Lampiran 6. Gambar Morfologi Mencit

Gambar 1. Kerontokan bulu bagian kepala dorsal

Gambar 2. Kebotakan bagian kepala dorsal

105

Gambar 3. Kerontokan Bulu Bagian Kepala ventral

Gambar 4. Kerontokan Bulu Bagian Kepala Ventral yang disertai kelumpuhan

106

Gambar 5. Kelumpuhan

107

Lampiran 7. Kegiatan Penelitian

Gambar 1. Alat yang digunakan dalam pembuatan ekstrak

Gambar 2. Penimbahan Hewan coba

108

Gambar 3. Pencekokan Ekstrak Daun Sirsak (Annona muricata L.)

Gambar 4. Persiapan Pembedahan

109

Gambar 5. Penandaan Area Mammae sebelum Pembedahan

Gambar 6. Organ Mencit dalam proses pembedahan

110

111