laporan inokulasi bakteri

7/26/2019 LAPORAN INOKULASI BAKTERI

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LAPORAN INOKULASI BAKTERILABORATORIUM MIKROBIOLOGI FARMASIJURUSAN FARMASIPOLITEKNIK KESEHATAN KEMENKKES MAKASSAR

LAPORAN INOKULASI BAKTERI

OLEH :KELOMPOK 4

KELAS REGULER A .

SITI ANIAH HARDIATY


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SRY REZKY AMALIAH SUPARMIN ROMI TUTI ERNAWATI VERA FEBRIANTI

YANTI SARI SYAM

JURUSAN FARMASIPOLITEKNIKKE SEHATAN KEMENKKES MAKASSAR

2011/2012

BAB I

PENDAHULUAN

I.1. LATAR BELAKANG

Dalam teknik biakan m !ni ti"ak #a$a "i%e!l kan ba&aimanamem%e!'le( # at biakan )an& m !ni* teta%i $ &a ba&aimana memeli(a!a#e!ta men+e&a( %en+ema!an "a!i l a!. In'k la#i "imak# "kan nt k men mb (kan* me!ema$akan mik!'ba "an men"a%atkan %'% la#i mik!'ba

)an& m !ni. In'k la#i a"ala( %eke!$aan memin"a(kan bakte!i "a!i me"i m)an& lama ke me"i m )an& ba! "en&an tin&kat ketelitian )an& #an&attin&&i. Me"ia nt k membiakkan bakte!i (a! #la( #te!il #ebel m"i& nakan. Pen+ema!an te! tama be!a#al "a!i "a!a )an& men&an" n&ban)ak mik!''!&ani#me. Pemin"a(an biakan mik!'ba )an& "ibiakkan

(a! # #an&at (ati,(ati "an memat (i %!'#e" ! lab'!at'!i m a&a! ti"ak te!$a"i k'ntamina#i. Ole( ka!ena it * "i%e!l kan teknik,teknik "alam%embiakan mik!''!&ani#me )an& "i#eb t "en&an teknik in'k la#i biakan.

Isol s! " #$%&! '%&() # * s( $( + & (*$(# '%'!s ,# * $ ( '%'!*- ,# *'!#&o" $%&$%*$( - &! l!*.#(*. * s%,!*.. -!)%&ol%, #(l$(& $ ( "! # * '(&*! -"%"%& ) + & ('(' *. - ) $ -!l #(# * -%*. * + & .o&%s * s$% # )l $% 3 + &$ "(& * $ ( $( *. )o(& )l $% 3 s%&$ '!#&o' *!)(l $o& $,% '!+&o' *!)(l $o&


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'%$,o-s l!'32001 S%+ & l '!3 " #$%&! -! l ' -!$%'(# * - l ' )o)(l s!+ ')(& * H * - l ' #% - * $%&$%*$( s 4 )o)(l s! !*! -!$%'(# * - l ' #% - *$%&$%*$( s 4 )o)(l s!!*! -!$%'(# * - l ' #% - * '(&*! U*$(# - ) $ '%')%l 4 &!s!5 $ "! # *3 'o&5olo.!3 - * s!5 $ 5 l!* 3 ' # o&. *!s'% *. # * -!$%l!$! , &(s

- ) $ -!)!s ,# * I*! "%& &$! " ,6 , &( s - "! # * '(&*! *. , *'%*. *-(*. s $( 4%*!s " #$%&! s 4

U*$(# '%')%&ol%, "! # * '(&*! - ) $ -!l #(# * )%*.%*+%& * -%*. *

'%*..(* # * " , * + !& $ ( ) - $ P - '(l * -!.(* # * .%l $!* s%" . ! " , *)%' - $ T%#*!# (*$(# '%')%&ol%, "! # * '(&*! - 7 + & 3 !$(8 $%#*!#)%*..o&%s * . &3 $%#*!# . & $( *.3 $%#*!# . & s%" &

T%#*!# !*o#(l s! '%&() # * s( $( )%#%&4 * '%'!*- ,# * " #$%&! - &!'%-!(' *. l ' #% '%-!(' *. " &( -%*. * $!*.# $ #%$%l!$! * *. s *. $$!*..! D%*. * -%'!#! * # * -!)%&ol%, "! # * '!#&oo&. *!s'% *. - ) $

-!.(* # * (*$(# )%'"%l 4 & * '!#&o"!olo.! P - )& #$!#(' !*! # * -!l #(# *$%#*!# !*o#(l s! "! # * '!#&oo&. *!s'% ) - '%-!(' s$%&!l (*$(# '%')%l 4 &!'!#&o"!olo.! -%*. * s $( #(l$(& '(&*! s 4

I-%*$!5!# s! "! # * '!#&oo&. *!s'% s%&!*.# l! '%'%&l(# * )%'!*- , * #%"! # * s%. & $ *) $%&4 -! )%*+%' & * P%'!*- , * '!#&oo&. *!s'% !*! -!l #(# *-%*. * $%#*!# s%)$!# (*$(# '%')%&$ , *# * #%'(&*! * "! # * s%l ' )%'!*- , *"%&(l *. # l! M!#&oo&. *!s'% - ) $ -!$('"(,# * - l ' "! # * + !& $ ( ) - $K%#%&(, * - l ' # l-( '%*(*4(## * $%&4 -!* )%&$('"(, * '!#&oo&. *!s'% B!l

'!#&oo&. *!s'% '%*(')(# ) - - s & $ "(*. ' # # * '%'"%*$(# s%-!'%*3s%- *.# * ) - )%&'(# * # l-( )%&$('"(, ** $%&l!, $ s%" . ! )%l!#%l

S%l !* - l ' '%-! + !&3 '!#&oo&. *!s'% 4(. '%')%&l!, $# * )%&$('"(, *-%*. * +!&! $%&$%*$( - l ' "! # * ) - $ s%)%&$! . & '!&!*. $ ( l%')%*. * . &A. & '!&!*. l 9!'* -!.(* # * (*$(# '%* !') * "! # * '(&*! s%- *.# * . & l%')%*. * l 9!'* -!.(* # * (*$(# '%'(&*!# * '!#&oo&. *!s'%

I 2 RUMUSAN MASALAH B . !' * + & '%*.!sol s! - * '%l!, $ '!#&oo&. *!s'% -!s%#!$ & #!$ - *

" . !' * + & '%*.!*o#(l s! '!#&oo&. *!s'% *. '(&*! - * '%l!, $'o&5olo.!* :

I 7 TUJUAN PRAKTIKUM U*$(# '%*.%$ ,(! - * '%' , '! + & '%*.!*o#(l s! '!#&oo&. *!s'%

U*$(# '%*. ' $! , s!l )%&%' 4 * '!#&oo&. *!s'%


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5/25

Teknik ini lebi( men& nt n&kan $ika "itin$a "a!i # " t ek'n'mi "anakt * teta%i meme!l kan ket!am%ilan,ket!am%ilan )an& "i%e!'le(

"en&an lati(an. Pen&&'!e#an )an& #em% !na akan men&(a#ilkan k'l'ni)an& te!%i#a(. In'k l m "i&'!e#kan "i %e!m kaan me"ia a&a! n t!ien

"alam +a aan %et!i "en&an $a! m %in"a( /l % in'k la#i0. Di anta!a &a!i#,&a!i# &'!e#an akan te!"a%at #el,#el )an& + k % te!%i#a( #e(in&&a "a%at

t mb ( men$a"i k'l'ni.a!a %en&&a!i#an "ilak kan %a"a me"i m %embiakan %a"at bent k

lem%en&. Bila "ilak kan "en&an baik teknik inila( )an& %alin& %!akti#.Dalam %en&e!$aann)a te!ka"an& be!be"a %a"a ma#in&,ma#in&lab'!at'!i m ta%i t $ ann)a #ama )aiit nt k memb at &'!e#an#eban)ak m n&kin %a"a lem%en& me"i m %embiakan. A"a bebe!a%ateknik "alam met'"e &'!e#an* )akni :

G'!e#an T +. G'!e#an !a"ian

b. Go&%s * #( -& * - Go&%s * s!* '"(*.


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Met'"e teba!Setete# in'k'l m "iletakan "alam #eb a( me"i m a&a! n t!ien "alam+a an %et!i"i#( "an "en&an men&& nakan batan& ka+a )an& ben&k'k "an#te!il. In'k la#i it "i#eba!kan "alam me"i m batan& )an& #ama "a%at"i& nakan "a%at men&in'k la#ikan %in&&an ke" a nt k "a%at men$amin%en)eba!an bakte!i )an& me!ata "en&an baik. Pa"a bebe!a%a %in&&anakan m n+ l k'l'ni k'l'ni )an& te!%i#a(,%i#a(.

Met'"e t an&I#'la#i men&& nakan me"ia +ai! "en&an +a!a %en&en+e!an. Da#a!melak kan %en&en+e!an a"ala( %en ! nan $ mla( mik!''!&ani#me

#e(in&&a %a"a # at #aat (an)a "item kan #at #el "i "alam tab n&/2ina!ni* 133 0.

Met'"e t # k Met'"e t # k )ait "en&an "en&an +a!a menete#kan ata men # kan$ n& $a! m '#e )an& "i"alamn)a te!"a%at in'k'l m* kem "ian

"ima# kkan ke "alam me"ia /2ina!ni* 133 0.

Pe!be"aan In'k la#i -am ! "an Bakte!i1. In'k la#i $am ! men&& nakan $a! m '#e bent k batan&. Hi5a )an&

be!bent k #e%e!ti benan& m "a( "iambil "en&an $a! m '#e batan& "anm "a( #ekali t mb ( "i "alam # at me"ia.

6. In'k la#i bakte!i men&& nakan $a! m '#e bent k b lat. Pa"a $ n& $a! m'#e )an& be!bent k b lat* bakte!i akan "a%at te!ambil "alam $ mla( )an&!elati5 ban)ak /R'(imat* 67760.

T%#*!# Isol s! D * P%'(&*! *T%#*!# !sol s! '!#&o" (*$(# '%'!s ,# * - * '%*.!-%*$!5!# s!3 4%*!s '!#&o" - &!"! # * - ) $ -!#l s!5!# s!# * "%&- s * s!5 $ )%&$('"(, * *. * ') # ) -'%-!

M%$o-% )%* * ' * ) - . &


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P - '%$o-% )%* * ' * ) - . &3 4!# s%-!#!$ s 4 s%l -!l%$ ## * - l ' '%-!('. &3 ' # $! ) s%l # * $('"(, '%*4 -! #olo*! *. $%&)!s , J!# s(s)%*s! s%l+(#() -!%*+% *3 #olo*! # * $%&)!s , -%*. * " !#3 s%,!*.. ' s!*.@' s!*.'%'!l!#! #%'(*.#!* * $!*..! (*$(# -!$(&(*# * '%*4 -! s%l $(*.. l N '(* (*$(#

'%'"( $ *. -%'!#! *3 )%*$!*. (*$(# '%*. '"!l s $( $!)% #olo*! *. -!!*.!*# *3'%' s(## * #% - l ' !& - * '%* * '* #%'" l! #% . & D%*. * '%*.(l *.!)&os%-(& !*! "%"%& ) # l! '%*4 '!* (*$(# '%')%&ol%, "! # * '(&*!

M%$o-% P%*.%*+%& *

M%$o-% *. s%-!#!$ - ) $ -!)%&+ - l , )%*.%*+%& * S(s)%*s! -!%*+% * s%&!- * +o*$o, ' s!*.@' s!*. )%*.%*+%& * -!$ * ' ) - . & J!# , * s%-!#!$+o*$o, - &! )%*.%*+%& * $%&$%*$( '%*(*4(## * )%&$('"(, *3 -!)%!& # * " ,6"%"%& ) - &! "! # * $ -! -!'(l ! - &! s%l $(*.. l M%$o-% !*! $!- # -!.(* # * #%+( l!

4!# )%* * ' * ) - . & $!- # -!'(*.#!*# * # &%* "%"%& ) l s * G '" & **. $!- # -!!*.!*# * - &! '%$o-% !*! - l , " ,6 '%$o-% !*! , * - ) $

-!.(* # * (*$(# !sol s! $!)% o&. *!s'% *. -o'!* * - l ' )o)(l s! + ')(& *

T%&- ) $ "%&" . ! + & '%*.!sol s! '!#&o" 3 !$(81. Isolasi Pada Agar Cawan

P&!*s!) ) - '%$o-% !sol s! ) - . & + 6 * - l , '%*.%*+% * '!#&oo&. *!s'%s%,!*.. -!)%&ol%, !*-!=!-( s)%s!%s *. - ) $ -!)!s ,# * - &! o&. *!s'% l !**s%$! ) #olo*! *. $%&)!s , *. $ ') # ) - + 6 * $%&s%"($ s%$%l , -!!*#(" s!"%& s l- &!s $( s%l $(*.. l T%&- ) $ "%"%& ) + & - l ' '%$o-% !sol s! ) - . & + 6 *3 !$(8 '%$o-% .o&%s #( -& * - * '%$o-% . & + 6 * $( *. B!l '%$o-%.o&%s #( -& * -!l #(# * -%*. * " !# # * '%*., s!l# * $%&!sol s!*'!#&oo&. *!s'%3 -!' * s%$! ) #olo*! "%& s l- &! s $( s%l

2. Isolasi Pada Medium Cair M%$o-% !sol s! ) - '%-!(' + !& -!l #(# * "!l '!#&oo&. *!s'% $!- # - ) $ $('"(,) - . & + 6 * '%-!(' ) - $ 3 $%$ )! , * - ) $ $('"(, ) - #(l$(& + !&M%$o-% !*! 4(. )%&l( -!l #(# * )%*.%*+%& * -%*. * "%"%& ) s%&! l )%*.%*+%& *S%' #!* $!*..! )%*.%*+%& * )%l( *. (*$(# '%*- ) $# * s $( s%l s%' #!* "%s &

3.

Isolasi Sel Tunggal M%$o-% !sol s! s%l $(*.. l -!l #(# * (*$(# '%*.!sol s! s%l '!#&oo&. *!s'%"%&(#(& * "%s & *. $!- # - ) $ -!!sol s! -%*. * '%$o-% . & + 6 */'%-!(' + !&S%l '!#&oo&. *!s'% -!l!, $ -%*. * '%*..(* # * )%&"%s & * s%#!$ & 100 # l!K%'(-! * s%l $%&s%"($ -!)!s ,# * -%*. * '%*..(* # * )!)%$ # )!l%& *. s *. $, l(s $ ()(* '!+&o' *!)(l $o&3 *. -!l #(# * s%+ & s%)$!s


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M + '@M + ' M%-!A- "%"%& ) ' + ' '%-! *. -!.(* # * (*$(# !*o#(l s! !$( 81 M! %- +(l$(&%8 "%&!s! -( $ ( l%"!, s)%s!%s '!#&oo&. *!s'%

2 Pl $% +(l$(&%8 '%-! ) - $ - l ' )%$&!-!s,

7 Sl *$ +(l$(&% 8 '%-! ) - $ - l ' $ "(*. &% #s!

S$ ) +(l$(&% 8 '%-! ) - $ - l ' $ "(*. &% #s!3 $%$ )! )%* * ' **

-%*. * + & )%*(s(# *

C

L! (!- +(l$(&% 8 '%-! + !& - l ' $ "(*. &% #s!> S, #% +(l$(&%8 '%-! + !& - l ' $ "(*. &% #s! *. )%* * ' ** -!#o+o#? & '%* %l!-!#! P! & * M(&*!D l ' #% - * s%"%* &* -! l ' "%" s "ol%, -!# $ # * $!- # - " #$%&! *.,!-() s%*-!&! $%&l%) s - &! s)%s!%s l !** K%& )# l! " #$%&! ) $o.%* #%- ) $ *"%&s ' @s ' " #$%&! s )&o" U*$(# '%* %*-!&!# * s( $( s)%s!%s - -!#%* l"%"%& ) + & 3 !$( 81 D%*. * )%*.%*+%& *


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S( $( s ')%l - &! s( $( s(s)%*s! *. "%&() + ')(& * "%&' + '@' + ' s)%s!%s-!%*+% * - l ' s( $( $ "(*. $%&s%*-!&! D &! %*+%& * !*! #%'(-! * -! '"!l s ')%l1 'l (*$(# -!%*+% * l%"!, l *4($ J!# - &! )%*.%*+%& * *. #%$!. !*! -! '"!l 031'l (*$(# -!s%" * ) - s( $( #olo*! $('"(, - l ' '%-!(' $%&s%"($3 $%$ )!'(*.#!* 4(. #!$ , * '%')%&ol%, s $( #olo*! s 4 D l ' , l *. -%'!#! * !*!#!$ '%')%&ol%, s $( #olo*! s 4 D l ' , l *. -%'!#! * !*! #!$ '%')%&ol%, s $(#olo*! '(&*! K l ( #!$ "%l(' #!*3 " ,6 #olo*! $(*.. l *. #!$ )%&ol%, !$('(&*!3 #!$ - ) $ '%*.(l *. )%*.%*+%& * -%*. * '%*..(* # * #olo*! !*! s%" . !s ')%l W l( o3 200C

2 D%*. * P%*( *. *Ro"%&$ #o+, 1; 7@;0C '%')(* ! '%$o-% *. l !*3 !$( -%*. * '%*. '"!ls%-!#!$ s ')%l + ')(& * " #$%&! *. s(- , -!%*+% *3 - * s ')%l !*! #%'(-! *-!s%" &l( s# * -! - l ' s( $( '%-!(' - &! # l-( - * .%l $!* %*+%& D%*. *-%'!#! * -!)%&ol%, , * l , s( $( )! & * -(# * S%$%l , '%-!(' !$( '%*.%*$ l3

' # s%l *. "%"%& ) 4 ' #%'(-! * * ') #l , #olo*! #olo*! *. ' s!*. ' s!*. - ) $ -! *.. ) '(&*! D%*. * '%*.(l *. )%#%&4 * s%)%&$! -! $ s3 ' ##,!&* # * -!)%&ol%, )! & * '(&*! *. l%"!, $%&4 '!* W l( o3 200C

7 D%*. * P%*..%s%# *M%$o-% !*! s%# & *. " * # -!.(* # *3 # &%* $!- # "%.!$( '%' # * 6 #$(3 , *s *.3 -%*. * + & !*! ' # " #$%&! * %&o" $!- # - ) $ $('"(, J!# (4(*. # 6 $!*o#(l s! -!"%*.#o# *3 #%'(-! * (4(*. # 6 $ !*o#(l s! !$( s%$%l , -!s%*$(,# *s( $( #olo*! l l( -!.%s%## * ) - )%&'(# * '%-!(' ) - $3 ' # "%"%& ) 6 #$(#%'(-! * #(& *. l%"!, 12 4 ' # * * ') #l , #olo*!@#olo*! *. l%$ #* $%&s%" & -! )%&'(# * '%-!(' J!# -! - # * )%'!*- , * s ')%l - &! s( $( #olo*! *.l%$ #* $%&)%*+!l3' # # * -!)%&ol%, s( $( )! & * '(&*! W l( o3 200C

D%*. * M%*.(+!l# * S $( S%l S!*.l% ?%ll Isol $!o* M!#&o)!)%$ - l , l $ *. - ) $ '%'(*.($ s $( " #$%&! - &! s%#! * " * #3 -%*. *$! - !#($ s%&$ * " #$%&! l !* M!#&o)!)%$ -!$%') $# * ) - $ *. * $ *. * s( $('!#&o' *!)(l $o& D%*. * '!#&o)!)%$ -!"( $ "%"%& ) $%$%s * "%&. *$(*. ) -s( $( # + )%*($() P%#%&4 * !*! -!l #( * -! " 6 , o" %#$!5 '!#&os#o) J!#$ ') # s( $( $%$%s * , * '%*. *-(*. s $( " #$%&!3 ' # -%*. * l !* '!#&o)!)%$3$%$%s * $%&s%"($ -!)!*- ,# * #% '%-!(' %*+%& -%*. * ' #s(- s() " #$%&!$%&s%"($ "%%'" *. "! # -(l( K%'(-! * - &! s!*! - ) $ -!)%&ol%, )! & * '(&*!M%o-% !*! s *. $ '%'%&l(# * #%s " & *3 l .!)(l '!#&o' *!)(l $o& s *. $ ' , lW l( o3 200C

C D%*. * I*o#(l s! H%6 *M%$o-% !*! -!- s * $ s s( $( #%* $ *3 " ,6 $!- # s%'( " #$%&! - ) $

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11/25

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