laporan praktikum 'teknik isolasi bakteri

8/16/2019 LAPORAN PRAKTIKUM 'Teknik Isolasi Bakteri'

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LAPORAN PRAKTIKUM

“TEKNIK ISOLASI BAKTERI DARI BAHAN PANGAN”

OLEH :

NAMA : RITA ANGGREANI WIDIASTUTI

NIM : D1C1 14 155

KELOMPOK : IV

KELAS : TPG-A 2014

JURUSAN ILMU DAN TEKNOLOGI PANGAN

FAKULTAS TEKNOLOGI DAN INDUSTRI PERTANIAN

UNIVERSITAS HALU OLEO

KENDARI

2016


2/15

I. PENDAHULUAN

I.1 Latar Belakang

Mi!""!#$%i&'( )(!&(*$! +,$& i $+$' +i%#,%#$% *$i i )$%$./

$i!/'$,,% ,$!$ K(*(!$$$% 'i!""!#$%i&'( *$!, $$) i)$ !$&$$%

'(+($)i'$$%$% 3$%# i)$ "%&,'&i $% &(*$#$i $i*$)%3$ !", $%#$%

$!$%# &($+i 3$%# &)(!i+ $% ,','%3$ )(!('$! "+(. *(!*$#$i 'i!""!#$%i&'(

$.$% $%#$%&(+$i% '(!,$$% &,'*(! #i7i *$#i '$%,&i$/ ,#$ &(*$#$i &,'*(!

'$$%$% *$#i (!('*$%#$% 'i!""!#$%i&'( P(!),'*,.$% $)$, (!('*$%#$%

'i!""!#$%i&'( $+$' '$$%$% &$%#$) (!$) .,*,%#$%%3$ (%#$% (.i,$%

'$%,&i$

Mi!""!#$%i&'( '(!,$$% '$.+, .i, 3$%# &$%#$) *$%3$/ *$i

i)$%$./ $i! '$,,% ,$!$ U%), i), (!+,%3$ i&"+$&i '$,,% (!',!%i$%

,%), '(%$$)$% 'i!""!#$%i&'( )(!&(*,) P",+$&i 3$%# *(&$! $% "'+(&

(%#$% *(!*$#$i 'i!"*$ )(!$$) $+$' ),*, '$%,&i$ )(!'$&, i',+,)/ &$+,!$%

(%(!%$$% $% ,+i) I&"+$&i $$+$. $!$ ,%), '('i&$.$% $)$, '('i%$.$%

'i!"*$ )(!)(%), $!i +i%#,%#$%%3$/ &(.i%##$ i(!"+(. ,+),! ',!%i $)$, *i$$%

',!%i K,+),! ',!%i i$+$. ,+),! 3$%# &(+-&(+ 'i!"*i$%3$ *(!$&$+ $!i

('*(+$.$% $!i &$), &(+),%##$+ K,+),! ',!%i $)$, *i$$% ',!%i i(!+,$%

$!(%$ &(',$ '()"( 'i!"*i"+"#i& 3$%# i#,%$$% ,%), '(%(+$$. $%

'(%#i(%)i8i$&i 'i!""!#$%i&'(/ )(!'$&, (%(+$$.$% i!i-i!i ,+),!$+/

'"!8"+"#i&/ 8i&i"+"#i&/'$,,% &(!"+"#i&/ '('(!+,$% &,$), ",+$&i 3$%# )(!i!i

$!i &$), '$$' 'i!""!#$%i&'( &$$

I.2 T!an


3/15

T,,$% $!i !$)i,' i%i 3$i), ,%), '(+$)i. !$)i$% $!$ '('*,$)

*i$$% ',!%i (%#$% '()"( (%#(%(!$% $% '()"( #"!(&


4/15

II. TIN"AUAN PUSTAKA

P",+$&i 'i!"*$ )i$ )(!i&$. &(%i!i '(%,!,) (%i&%3$/ )()$i )(!i!i $!i

$',!$% *(!*$#$i '$$' &(+ P",+$&i *$)(!i i +$*"!$)"!i,' $$) ii&"+$&i

'(%$i ,+),! ',!%i 3$%# )(!i!i $!i &$), (%i& 3$%# $$) i(+$$!i '"!8"+"#i/

&i8$) $% ('$',$% *i"i'i$i%3$ I&"+$&i '(!,$$% $!$ ,%), '('i&$.$%

$)$, '('i%$.$% 'i!"*$ )(!)(%), $!i +i%#,%#$%%3$/ &(.i%##$ i(!"+(.

,+),! ',!%i $)$, *i$$% ',!%i K,+),! ',!%i i$+$. ,+),! 3$%# &(+-&(+

'i!"*$%3$ *(!$&$+ $!i ('*(+$.$% $!i &$), &(+ ),%##$+ 9Ti' P(%3,&,%/ 2014

P((!$$% '('i%$.$% 'i!"*$ $!i '(i,' 3$%# +$'$ ( '(i,'

3$%# *$!, .$!,& i+$,$% &($!$ )(+i)i T(!+(*i. $.,+, .$!,& i,&$.$$% $#$!

&(',$ $+$)-$+$) 3$%# $$ &$%#,) $,)%3$ (%#$% '(i,' $% ((!$$% i%",+$&i

i), *(%$!-*(%$! &)(!i+ H$+ i%i ,%), '(%#.i%$!i "%)$'i%$&i/ 3$i), '$&,%3$

'i!"*$ 3$%# )i$ ii%#i%$% 9Ti' P(%3,&,%/ 2014

M(i$ (!),'*,.$% 9i&i%#$) '(i,' $$+$. )('$) ,%),

'(%,'*,.$% 'i!"*$ Mi!"*$ '('(!+,$% %,)!i&i ,%), '('(%,.i (*,),.$%

(%(!#i $% ,%), *$.$% ('*$%#,% &(+/ ,%), &i%)(&$ !")"+$&'$ $% *$#i$%-

*$#i$% &(+ +$i% S()i$ 'i!"*$ '(',%3$i &i8$) 8i&i"+"#i )(!)(%),/ &(.i%##$

'('(!+,$% %,)!i&i )(!)(%), ,+$ S,&,%$% i'i$ &(+ 'i!"*$ !(+$)i8 )()$/ *$i

,%&,! i'i$ '$,,% &(%3$$ 3$%# )(!$%,%# i $+$' &(+ D$!i .$&i+ $%$+i&i&

i'i$ i()$.,i *$.$ (%3,&,% ,)$'$ &(+ $$+$. ,%&,! i'i$ C/ H/ O/ N/ $% P/

3$%# ,'+$.%3$ ;0-


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)(!i!i $!i !")"+$&'$/ i%i%# &(+/ +ii$ ,%), $$%#$% '$$%$%/

"+i&$$!i$/ "+i8"&8$)/ $% &(%3$$ +$i% 9Li'/ 2006

T(%i i&"+$&i 'i!""!#$%i&'( $$+$. &,$), ,&$.$ ,%), '(%,'*,.$%

'i!"*$ i+,$! $!i +i%#,%#$% $+$'i$.%3$ P('i&$.$% 'i!""!#$%i&'( $!i

+i%#,%#$% i%i *(!),,$% ,%), '('(!"+(. *i$$% *$)(!i 3$%# &,$. )i$

*(!$',! +$#i (%#$% *$)(!i +$i%%3$ $% i&(*,) *i$$% ',!%i P!i%&i $!i

i&"+$&i 'i!"*$ $$+$. '('i&$.$% &$), (%i& 'i!"*$ (%#$% 'i!"*$ +$i%%3$

3$%# *(!$&$+ $!i $',!$% *(!'$$'-'$$' 'i!"*$ H$+ i%i $$) i+$,$%

(%#$% '(%,'*,.$%%3$ $+$' '(i$ $$)/ &(+-&(+ 'i!"*$ $$% '('*(%),

"+"%i &(+ 3$%# )()$ $$ )('$)%3$ 9N,! $% A&%$%i/ 200?

T(!$$) *(*(!$$ $!$ ,%), '(%#i&"+$&i 'i!"*$ 3$%i )(%i i!i%#$%

#"!(&$% 9Streak plate method / *(!),,$% ,%), '(+()$$% &(*$#i$% *(&$!

"!#$%i&'( $$ *(*(!$$ #"!(&$% (!)$'$ $% '()"( ),$%# 9 pour-plate method /

$',!$% i), (',i$% i),%#$% ($+$' $$% ()!i &)(!i+ $% i*i$!$% $$)

(!),'*,.$% "+"%i )(!$i *$i $+$' '(i,' ),,$% $$ (,$ !"&(& i$+$.

,%), '('i&$.$% *$)(!i &$), &$'$ +$i% &(.i%##$ &(+-&(+ i), $$% ),'*,.

'(%$i "+"%i-"+"%i 3$%# )(!i&$. i$+$' '(i,' 3$%# $$) 9Di3$%$/

2011

P(%#(%(!$% $$+$. '(+$!,)$% $)$, '(+($&$% 'i!"*$ $!i &,*&)!$)%3$

( $+$' $i! &(.i%##$ +(*i. ',$. (%$%#$%$%%3$ T,,$% (%#(%(!$% 3$i),

,%), '(%#,!$%#i ($$)$% *$)(!i 3$%# i)$%$' 9F$i&/ 200


6/15

III. METODOLOGI PRAKTIKUM

#.1. Te$%at &an 'akt

P!$)i,' i+$&$%$$% i L$*"!$)"!i,' A#!")(%"+"#i U%i) Fi)"$)"+"#i/

F$,+)$& P(!)$%i$% $$ .$!i S(+$&$/ 10 M(i 2016 ,,+ 100-1500 WITA

#.2. Ba(an &an Alat

$.$% 3$%# i#,%$$% $+$' !$)i,' i%i 3$i), '(i$ NA/ $B,$(&/

yoghurt / i$% *,&,/ '$$%$% $$%$%/ $i! 'i%(!$+ i&i ,+$%# $% $+"."+

A+$) 3$%# i#,%$$% $+$' !$)i,' i%i 3$i), )i'*$%#$% $%$+i)i/ +$',

,%&(%/ (%3(*$! glass rod / $$% ()!i/ 'i!"),*(/ 'i!"i()/ laminary air

flow $% E!+(%'(3(!

#.#. Pr)*e&r Ker!a

P!"&(,! (!$ !$)i,' i%i 3$i), &(*$#$i *(!i,) :

Tekn+k agar *e,ar

1 Si$$% > *,$. 'i!"),*( *(!i&i 0< '+ $i! &)(!i+2 Pi() 01 '+ &,&(%&i &$'(+ $!i E!+(%'(3(! $% $',!$% ($+$'

'i!"),*( *(!i&i $i! &)(!i+

G"3$%# $% ,)$! 'i!"),*( &(.i%##$ )(!$',! (%#$% *$i $%

+$,$% (%#(%(!$% *(!&(!i .i%##$ 'i!"),*( (->

4 C(+,$% (%3(*$! 9 glass rod ( $+$' $+"."+/ +$+, $%$&$%

(%3(*$! .i%##$ $+"."+ )(!*$$! .$*i&

5 Di%#i%$% (%3(*$! &(*(+,' i#,%$$%

6 Pi() 01 '+ $i!$% &,&(%&i *$)(!i $!i 'i!"),*( ? $% > (%#$%

'i!"i() &($!$ )(!i&$.

? T,$%# &,&(%&i *$)(!i $!i '$&i%#-'$&i%# 'i!"),*( $+$' $#$!

+('(%#$% $% &(*$! (%#$% '(%##,%$$% *$)$%# (%3(*$! .i%##$ !$)$

$% (!i%#

> Si'$% *i$$% ( $+$' i%,*$)"!


7/15

I-. HASIL DAN PEMBAHASAN

.1. Ha*+l

G$'*$! "+"%i *$)(!i 3$%# ),'*,. $$ '$&i%#-'$&i%# &$'(+

T$*(+ 1 H$&i+ (!.i),%#$% ,'+$. "+"%i $% ",+$&i *$)(!i

/a0an %enga$atan "$la( k)l)n+ t$,( P)%la*+ ,akter+$l

C$$% C 10-? 2>4 2>4 1010 CFU'+

C$$% C 10-> 4> 4> 1011 CFU'+

C$$% D 10-? 164 164 1010 CFU'+

C$$% D 10-> 26? 26? 1011 CFU'+

C$$% A 10-? ?1 1426 1011 CFU'+

C$$% A 10-> 20 64 1011 CFU'+

.2. Pe$,a(a*an

I&"+$&i '(!,$$% $!$ ,%), '('i&$.$% $)$, '('i%$.$% 'i!"*$

)(!)(%), $!i +i%#,%#$%%3$/ &(.i%##$ i(!"+(. ,+),! ',!%i $)$, *i$$% ',!%i

A : &$'(+ ,( 910->

: &$'(+ i$% 910->

C : &$'(+ 3"#.,!) 910->

D : &$'(+ $i! 910->


8/15

K,+),! ',!%i i$+$. ,+),! 3$%# &(+-&(+ 'i!"*$%3$ *(!$&$+ $!i ('*(+$.$% $!i

&$), &(+ ),%##$+

T(%i $#$! ),$%# i+$,$% (%#(%(!$% &$), '$)$ +, &,&(%&i *$)(!i

i$+$' )i#$ )$*,%# $i! &)(!i+ &(.i%##$ $$% i(!"+(. $#$! +('(%#$% (%#$%

,'+$. *$)(!i 3$%# ")i',' ,%), i&"+$&i T(%i i%i +(*i. ',$. $!(%$ ,%),

'(%$$)$% ,'+$. "+"%i 3$%# )(!i&$. )i$ i(!+,$% ()(!$'i+$% &((!)i

$$ )(%i (%##"!(&$%

I&"+$&i 'i!"*i")$ 3$%# i+$,$% $+$' !$)i,' i%i $$+$. (%#$% $!$

(%#(%(!$% 9dilution/ 3$%i )(%i (%#(%(!$% *(!)i%#$) T,,$% $!i

(%#(%(!$% *(!)i%#$) 3$i), '('(!(i+ $)$, '(%#,!$%#i ,'+$. 'i!"*$ 3$%#

)(!&,&(%&i $+$' $i!$% P(%(%),$% *(&$!%3$ $)$, *$%3$%3$ )i%#$)

(%#(%(!$% )(!#$%),%# ($$ (!i!$$% ,'+$. 'i!"*$ $+$' &$'(+

P(%(%),$% ,'+$. *$)(!i $$) i+$,$% '(+$+,i (%#.i),%#$% ,'+$.

*$)(!i 3$%# .i, 9viable count P(%#.i),%#$% i&(*,) ,#$ &(*$#$i standard

plate count, 3$%# i$&$!$% $$ $&,'&i *$.$ &()i$ &(+ *$)(!i 3$%# .i,

$+$' &,&(%&( $$% ),'*,. '(%$i &$), "+"%i &()(+$. ii%,*$&i $+$' '(i$

*i$$% (%#$% +i%#,%#$% 3$%# &(&,$i S()(+$. '$&$ i%,*$&i/ ,'+$. "+"%i

3$%# ),'*,. i.i),%# $% '(!,$$% (!i!$$% $)$, ,#$$% $!i ,'+$. *$)(!i

$+$' &,&(%&i J,'+$. *$)(!i '(!,$$% &$+$. &$), 8$)"! (%)i%# ,%),

i()$.,i/ $!(%$ $$) '(%(%),$% i%(!$ $!i *$)(!i )(!&(*,)

M(i$ 3$%# i#,%$$% $+$' '(%,'*,.$% *$)(!i $$+$. '(i$ NA

Nutrient Agar 9NA '(!,$$% &,$), '(i,' 3$%# *(!*(%), $$)/ 3$%#

'(!,$$% (!$,$% $%)$!$ *$.$% $+$'i$. $% &(%3$$-&(%3$$ i'i$ NA

i*,$) $!i $',!$% (&)!$ $#i%# $% ()"%( (%#$% '(%##,%$$% $#$!


9/15

&(*$#$i ('$$) D$+$' .$+ i%i $#$! i#,%$$ &(*$#$i ('$$)/ $!(%$ &i8$)%3$

3$%# ',$. '('*(, $% '(%#$%,%# $!*".i!$) 3$%# *(!,$ #$+$)$'

&(.i%##$ )i$ ',$. i,!$i$% "+(. 'i!""!#$%i&'( D$+$' .$+ i%i (&)!$ *((8

$% ()"% i#,%$$% &(*$#$i *$.$% $&$! $!(%$ '(!,$$% &,'*(! !")(i%/

%i)!"#(%/ @i)$'i% &(!)$ $!*".i!$) 3$%# &$%#$) i*,),.$% "+(. 'i!""!#$%i&'(

,%), ),'*,. $% *(!('*$%# M(i,' Nutrient Agar 9NA '(!,$$% '(i,'

3$%# *(!$!%$ "+$) ',$ 3$%# '('i+ii "%&i&)(%&i 3$%# $$) i'$%$

'(i,' i%i *(!$&$+ $!i &i%)()i $% '('i+ii (#,%$$% &(*$#$i '(i,' ,%),

'(%,'*,.$% *$)(!i

H$&i+ (%#$'$)$% ,'+$. "+"%i 'i!"*$ $% ",+$&i 'i!"*$ i(!"+(.

$!i (&(+,!,.$% &$'(+ *$.$ ,'+$. "+"%i )(!)i%##i )(!$$) $$ &$'(+ ,(

(%#(%(!$% (-? (%#$% ,'+$. "+"%i ?1 (%#$% ",+$&i *$)(!i &(*(&$!

1426 1011 CFU'+ J,'+$. "+""%i 3$%# !(%$. )(!$$) $$ &$'(+ $i! i&i

,+$%# (%#(%(!$% (-? (%#$% ,'+$. "+"%i 164 (%#$% ",+$&i *$)(!i

&(*(&$! 164 1010 CFU'+


10/15

-. PENUTUP

.1. Ke*+$%lan

P!$)i,' )(%i i&"+$&i *$)(!i i+$,$% '(%##,%$$% )(%i $#$! &(*$!

$% i(!"+(. ",+&$i *$)(!i 3$%# ),'*,. $$ &$'(+ 3"#.,!) 10-? 3$i), 2>4

10?/ &$'(+ 3"#.,!) 10-> 3$i), 4> 1011/ &$'(+ $i! 10-? 3$i), 164 1010/ $%

&$'(+ $i! 10-> 3$i), 26? 1011

.2. Saran

P!$)i,' i%i &$%#$) i(!+,$% (.$)i-.$)i$%/ )(!,)$'$ $+$' '(+$,$%

)(%i $#$! &(*$! $!(%$ i$ )i$ .$)i-.$)i/ '(i$ 3$%# )(!$$) $+$' $$%

$$) !()$ &(.i%##$ .$&i+%3$ )i$ '$&i'$+


11/15

DA3TAR PUSTAKA

D3$%$ / N,!.$($! 2011 Mikrobiologi Dasar U%i@(!&i)$& H$&$%,i%

M$$&&$!

Li'/D 2006 Microbiology MG!$-Hi++ N( "!

N,!".$i$%$. 200? Media UI P!(&& J$$!)$

N,! I%!i3$%i/ A&%$%i 200? Penuntun Praktikum Mikrobiologi Akuatik / F$,+)$&

P(!i$%$% $% I+', K(+$,)$% U%i@(!&i)$& H$+, O+(" K(%$!i

Ti' P(%3,&,% 2014 Petunuk Praktikum M!"! Mikrobiologi PertanianU%i@(!&i)$& S(*(+$& M$!() S,!$$!)$


12/15

LAMPIRAN

P)%la*+ ,akter+ 4 "K 5 3. Pengen6eran 5 **%en*+ %a&a *aat $en7e,ar

• "#.,!) 910-?

JK F P(%#(%(!$% &,&(%&i $$ &$$) '(%3(*$!

2>4 10? 10

2>40 10?

2>4 1010 CFU'+

• "#.,!) 910->

JK F P(%#(%(!$% &,&(%&i $$ &$$) '(%3(*$! 4> 10> 10

4>0 10>

4> 1011 CFU'+

• Ai! i&i ,+$%# 910-?

JK F P(%#(%(!$% &,&(%&i $$ &$$) '(%3(*$!

164 10? 10

1640 10?

164 1010 CFU'+• Ai! i&i ,+$%# 910->

JK F P(%#(%(!$% &,&(%&i $$ &$$) '(%3(*$!

26? 10> 10

26?0 10>

26? 1011 CFU'+

• K,( 910-?

JK F P(%#(%(!$% &,&(%&i $$ &$$) '(%3(*$!

?1 10? 20

14260 10?

1426 1011 CFU'+

• K,( 910->

JK F P(%#(%(!$% &,&(%&i $$ &$$) '(%3(*$!

20

?

?


13/15

260 10? 20

5200 10?

52 1010 CFU'+

• I$% *,&, 910->

JK F P(%#(%(!$% &,&(%&i $$ &$$) '(%3(*$!

20 10> 20

6400 10>

64 1011 CFU'+


14/15


15/15

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