Lesson Plan PTT 202/3 Sem I 2012/13

UNIVERSITI MALAYSIA PERLIS (UniMAP) Pusat Pengajian Kejuruteraan Bioproses

COURSE OUTLINE

(1) Kod kursus/ Course code: PTT 202

(2) Tajuk kursus/ Course title: Organic Chemistry for Biotechnology /Kimia Organik untuk Bioteknologi

(3) Nilai unit/ Number of unit: 3

(4) Jenis kursus/ Course type: Teras/ Core

(5) Prasyarat/ Prerequisite: Tiada/ None

(6) Sinopsis kursus/ Course synopsis:

This course is to introduce students with basic principles and instrumentation in analytical bio-organics

methods that can be applied in biotechnology related fields. The course covers analytical techniques

commonly used in biochemical works which categorized under spectroscopy, chromatography, electro-

analytical and radioactivity based methods. The course also discusses on enzymes, antibodies,

radioisotopes and substances which may be necessary to detect and measure biochemical compounds

or which can be very useful in variety of analytical methods. The course is complemented by explanation

on chemical nature, isolation-separation techniques and methods of analysis of carbohydrates, amino

acids, proteins, lipids and nucleic acids.

(7) Hasil Pembelajaran / Learning Outcomes:

At the end of the course, students are expected to acquire these abilities:

i) Ability to apply the basic principles and calculations of analytical and separation methods for

qualitative and quantitative analysis.

ii) Ability to describe and analyze the use of biological materials for analytical purposes.

iii) Ability to differentiate and compare analytical methods for the separation and purification of

biomolecules.

Lesson Plan PTT 202/3 Sem I 2012/13

(8) Senarai eksperimen / List of experiments:

Lab 1 To introduce laboratory equipment and safety

Lab 2 To characterize an unknown compound using Fourier Transform Infra Red (FTIR) I

Lab 3 To characterize an unknown compound using Fourier Transform Infra Red (FTIR) II

Lab 4 To determine limonene concentration by gas chromatography (GC) I

Lab 5 To determine limonene concentration by gas chromatography (GC) II

Lab 6 To separate organic compound using thin layer chromatography (TLC) technique I

Lab 7 To separate organic compound using thin layer chromatography (TLC) technique II

Lab 8 To determine an active drug substance in a pharmaceutical formulation using ultraviolet and

visible spectrometry (UV/VIS)

Lab 9 To extract and detect a lipid I

Lab 10 To extract and detect a lipid II

Lab 11 To immobilize an enzyme

Lab 12 To apply column chromatography (CC) technique to separate phenolic acids

(9) Pendekatan pembelajaran/ Learning approach:

i) Lecture : 28 hours (40 %)

ii) Laboratory : 42 hours (60 %)

(10) Sumbangan penilaian / Evaluation contribution:

(i) Peperiksaan/ Examination: 60%

Mid Term Examination = 20 %

Final Examination = 40 %

(ii) Kerja Kursus/Course work: 40%

Assignments & Quizzes = 10 %

Laboratories = 30 %

(11) Pensyarah dan Pegawai Latihan Vokasional / Lecturers and Teaching Engineers:

Lecturers: Teaching Engineers/PLV:

i) En. Zulkarnain Mohamed Idris i) En. Ahmad Radi Wan Yaakub

ii) Cik Nor Helya Iman Kamaludin ii) En. Mohd Qalani Che Kassim

(12) Senarai buku teks dan rujukan / List of text books and references :

i) Gary D. Christian. Analytical Chemistry. 6th Edition. Publisher: John Wiley & Sons, Inc, 2004.

ii) Holme. D. J. and Peck. H., Analytical Biochemistry 3rd ed. Pearson education limited, 1998.

iii) Manz. A., Pamme. N. Lossifidis. D. Bioanalytical Chemistry, Imperial College Press, London, 2004.

iv) Mikkelsen, Susan R., Corton. E. Bioanalytical chemistry. John Wiley & Sons, Inc. Hoboken, New

v) Edmond de Hoffmann, Vincent Stroobant, Mass Spectrometry: Principles and Applications

Wiley-Interscience, 2007.

vi) Shiqi Peng, Ming Zhao, Pharmaceutical Bioassays: Methods and Applications, Wiley, 2009.

Lesson Plan PTT 202/3 Sem I 2012/13

RANCANGAN MENGAJAR/LESSON PLAN

Minggu/Week Kandungan Kursus / Course Contents

(Panduan/Guidelines)

Pensyarah/Lecturer

Week 1

(10 -14 Sept

2012)

General Principles of Analytical Organic Chemistry for

Biotechnology

Selection of valid methods of analysis, quantity of data

and production result.

(2 hours)

En. Zulkarnain

Lab 1: Introduction to laboratory equipment and safety

(3 hours)

En. Ahmad Radi

En. Qalani

Week 2

(17 -21 Sept

2012)

Spectroscopy

Interaction of radiation with matter; Molecular

absorptiometry; Absorptiometry design; Molecular

fluorescence techniques; Atomic spectroscopy

techniques; Magnetic resonance spectroscopy.

(2 hours)

En. Zulkarnain

Lab 2: Characterization of an unknown using Fourier

Transform Infra Red (FTIR) I

(3 hours)

En. Ahmad Radi

En. Qalani

Week 3

(24-28 Sept

2012)

Separation Methods

Principle of separation techniques; Methods based on

polarity, ionic nature, size and shape.

(2 hours)

En. Zulkarnain

Lab 3: Characterization of an unknown using Fourier

Transform Infra Red (FTIR) II

(3 hours )

En. Ahmad Radi

En. Qalani

Week 4

(1-5 Oct 2012)

Electroanalytical Methods

Potentiometry, Conductometry, Coulometry, Voltametry

and Biosensors.

(2 hours)

En. Zulkarnain

Lab 4: Determination of limonene concentration by gas

chromatography I

(3 hours)

En. Ahmad Radi

En. Qalani

Week 5

(8-12 Oct

2012

Radio Isotopes

Nature of radioactivity, detection and measurement of

radioactivity; Biochemical used of isotopes.

(2 hours)

En. Zulkarnain

Lab 5: Determination of limonene concentration by gas

chromatography II

(3 hours)

En. Ahmad Radi

En. Qalani

Lesson Plan PTT 202/3 Sem I 2012/13

Week 6

(15-19 Oct

2012

Atomated Methods of Analysis

Discrete analysis; Flow analysis; Robotics.

(2 hours)

En. Zulkarnain

Lab 6: Separation of organic compound using thin layer

chromatography (TLC) technique I

(3 hours)

En. Ahmad Radi

En. Qalani

Week 7

(22-25 Oct

2012

Immumological Methods

General processes of the immune response; Antigen-

antibody reactions; precipitation reactions and

immunoassay.

(2 hours)

En. Zulkarnain

Lab 7: Separation of organic compound using thin layer

Chromatography (TLC) technique II

(3 hours)

En. Ahmad Radi

En. Qalani

Week 8

(26 Oct-4 Nov

2012)

Cuti Pertengahan Semester/ Mid-term Break

Week 9

(5-9 Nov

2012)

Enzymes

Enzyme in biological reactions; Catalytic antibodies and

artificial enzymes; Enzyme-catalyzed reactions and

immobilized enzymes.

(2 hours)

Cik Nor Helya Iman

Lab 8: Determination of active drug substance in a

pharmaceutical formulation using ultraviolet and visible

spectrometry (UV/VIS)

(3 hours)

En. Ahmad Radi

En. Qalani

Week 10-11

(12-23 Nov

2012)

Carbohydrates

Carbohydrate analysis; Enzymatic methods of

carbohydrates analysis; Separation and identification of

carbohydrates mixtures.

(4 hours)

Cik Nor Helya Iman

Lab 9: Extraction and detection of lipid I

(3 hours)

En. Ahmad Radi

En. Qalani

Lab 10: Extraction and detection of lipid I

(3 hours)

Week 12

(26-30 Nov

2012)

Amino acids

Separation of amino acid mixtures; Amino acid analyzer;

Resolution of racemic mixtures of amino acids.

(2 hours)

Cik Nor Helya Iman

Lesson Plan PTT 202/3 Sem I 2012/13

Nota Penting: Kehadiran pelajar ke kuliah/tutorial/makmal adalah WAJIB. Jika tidak hadir tanpa

sebab, pelajar boleh dihalang daripada menduduki peperiksaan akhir.

Lab 11: Immobilization of enzyme

(3 hours)

En. Ahmad Radi

En. Qalani

Week 13

(3 -7 Dec

2012)

Proteins

Separation of proteins and protein denaturation.

(2 hours)

Cik Nor Helya Iman

Lab 12: To apply column chromatography (CC)

technique to separate phenolic acids

(3 hours)

En. Ahmad Radi

En. Qalani

Week 14

(10 -14 Dec

2012)

Lipids

Separations of lipid mixtures; Biosynthesis of terpenes and

cholesterols; Synthetic steroid

(2 hours)

Cik Nor Helya Iman

Week 15

(17-21 Dec

2012)

Nucleic acids

Isolation and purification of nucleic acids; Methods of

nucleic acid analysis and DNA sequencing.

(2 hours)

Cik Nor Helya Iman

Week 16

(24-30 Dec

2012)

Minggu Ulangkaji/ Study Week

Week 17-19

(31 Dec-20

Jan 2013)

Peperiksaan Akhir Semester/ Final Examination

Lesson Plan PTT 202/3 Sem I 2012/13

LABORATORY GROUPING PTT 202 ORGANIC CHEMISTRY FOR BIOTECHNOLOGY

Group 1

AHMAD KHAIRUL ANUAR BIN RAMLI (L)

FATEN SYAMIMI BINTI KAMYIS

NOR AMIRRA BINTI ABU NAYAN

NURSYAFIQAH ATHIRAH BINTI MOHAMAD

ZABIDI

Group 2

AIMI SHAZWANI BINTI SHARIFFUDDIN (L)

FATIN IZZATI BINTI MOHMED AZMI

NOR SYAZWANI BINTI AZMAN

NURUL SAKINAH BINTI MOHD NOR

Group 3

AMINAH BINTI IDERIS (L)

KUEK SZE TENG

NORAINON MAULAD NASHIR

SITI MARIAM BINTI MAZALAN

WAN ASMA SALSABILA BINTI WAN MOHD NOR

Group 4

ZAFRYL ZAHEIDY BIN MUSTOFA (L)

ASMALAILI BINTI SALEHUDIN

LAU SIN MUN

NUR AZREEN BINTI ARIFFIN

SITI NORAFFIYAH BINTI MONSHI

Group 5

AZZAHRA BINTI ARIPIN (L)

MEOR AZEEM BIN MEOR AZLI

NUR FARAHIN BINTI ROSTAM

SITI NURULNASYRA ATHIRA BINTI AZMAN

Group 6

TAN CHAI YUN (L)

ENIS NATASHA NOOR BINTI ARBAAIN

NOOR SAFIRA AINA BINTI ARSHAD

NUR SYAZANA BINTI JALANI

(L)= Leader

Lesson Plan PTT 202/3 Sem I 2012/13

RANCANGAN MAKMAL & TUTORIAL / LABORATORY PLAN & TUTORIAL

Week/Group Group 1 – 6

1

14/9 Lab 1: EXP 1

2

21/9 Lab 2: EXP 2

3

28/9 Lab 3: EXP 3

4

5/10 Lab 4: EXP 4

5

12/10 Lab 5: EXP 5

6

19/10 Lab 6: EXP 6

7

26/10 Lab 7: EXP 7

8

2/11 Mid Semester Break

9

9/11 Lab 8: EXP 8

10

16/11 Lab 9: EXP 9

11

23/11 Lab 10: EXP 10

12

30/11 Lab 11: EXP 11

13

7/12 Lab 12: EXP 12

14

14/12 Lab Test

Nota Penting: Kehadiran pelajar ke kuliah/tutorial/makmal adalah WAJIB. Jika tidak

hadir tanpa sebab, pelajar boleh dihalang daripada menduduki peperiksaan akhir.