jurnal pemeriksaan fungsi hati 2

7/26/2019 Jurnal Pemeriksaan Fungsi Hati 2

1/10

JURNAL

KESEHATAN

KELAINAN FUNGSI HATI DAN GINJAL TIKUS PUTIH (Rattus norvegicus,L.)AKIBAT SUPLEMENTASI TAWAS DALAM PAKAN

Ratih HaribiSri Darmawati

Tri Hartiti

ABSTRACT

Alum is used to improve the quality oood containing to!ic heavy metal ions "hich can interere "ithaluminum en#ymatic system, and tissue damage. Liver and $idney are the most used net"or$ is aected,%ecause it is a deto!iication organ. Liver and $idney damage can %e detected %y an e n#yme concentration oS&'T, S&T, Billiru%in,rotein, reum and Creatinin in the %lood

This study aims to ind out the eects o alum in aeed supplementor liver and $idney damage in a

clinic conducted rom *ay to 'cto%er +-, at the La%oratory o the niversity Clinic atology

*uhammadiyah Semarang. Sample studies o "hite rats (Rattus norvegicus,L.), aged + months "ith "eight

average o + grams. dose treatment ("ithout supplementation), ./, .0, ,.+, ./, 0 and

("ithout supplementation), and su%sequent treatment "ith a dose o +, 1, 2, / and 3 alum, "ho

every day put into the stomach o rats 0 ml. Clinical la%oratory tests perormed at the time %eore treatment

(control), 2 "ee$s, 3 "ee$s and 4 "ee$s o e!posure time. 5!amination AST and ALT "ith ltra 6iolet Test

methods, Total Billiru%in "ith modiications &ro 7endrasi$ method, total protein Colorimetri method,

Berthelot method, Creatinin 7ae method

Clinical chemistry tests sho"ed that supplementation inluence o alum on the concentration oen#ymes and other actors in the %lood o mice associated "ith damage to liver and $idney tissue.Level oorgan damage signiicans "ith alum in a eed supplement. The higher the concentration o alumdisuplementasi$an and the longer e!posure time resulted in damage to the liver and $idneys getting "orse.

ABSTRA8Tawas yan !i"na#an "nt"# $%nin#atan m"t" ma#anan m%nan!"n i&n '&am b%rat tsi# yait"

a'"mini"m yan !a$at m%nan" syst%m %n(imati#) !an m%r"sa# *arinan+ Hati !an in*a' a!a'ah *arinanyan $a'in !"'" t%r#%na !am$a# t%rs%b"t) #ar%na m%r"$a#an &ran !%tsi,i#asi+ K%r"sa#an hati !an in*a'!a$at !i!%t%#si !%nan $%m%ri#saan #&ns%ntrasi %n(im S--T) SGPT) Bi''ir"bin) Pr&t%in) Ur%"m !an

.r%atinin !a'am !arah+P%n%'itian ini b%rt"*"an m%n%tah"i %,%# s"$'%m%ntasi tawas !a'am $a#an t%rha!a$ #%r"sa#an hati

!an in*a' s%/ara #'ini# yan !i'a#"#an m"'ai M%i sam$ai !%nan -#t&b%r 0112) !i Lab&rat&ri"m Pat&'&yK'ini# Uni3%rsitas M"hamma!iyah S%maran+ Sam$'% $%n%'itian b%r"$a ti#"s $"tih (Rattus norvegicus, L.),"m"r 0 b"'an !%nan b%rat ba!an rata4rata 011 ram + D&sis $%r'a#"an 15 6tan$a s"$'%m%ntasi7) 1)185)1)95) 1)05) 1)85) 95 !an 15 6tan$a s"$'%m%ntasi7) !an $%r'a#"an s%'an*"tnya !%nan !&sis 05) :5) ;5)

/ !an


2/10

J"rna' K%s%hatan

K%'ainan F"nsi Hati Dan Gin*a' Ti#"s P"tih (Rattus norvegicus) L+) A#ib a9S"$9%m%ntasiA

Tawas Da'am Pa#an

PEN GANT AR

Tawas banya# !i"na#an "nt"#

m%m$%r bai#i m"t" ma#anan) s%$%rti $a!a

$%n&'ahan 'i!ah b"aya s%b%'"m !i&'ah

m%n*a!i ma#anan) #"'itnya !i#"$as)

!ir%mas !%nan aram !an !ir%n!am !a'am

air yan !i/am$"r tawas aar ba" !an

'%n!irnya hi'an

I#an s%b%'"m !iasa$ !ir%n!am

!a'am 'ar"tan tawas 915 s%'ama '*am)

t%#st"rnya #%nya') $"tih) rasa $ahit !anamisnya b%r #"ran 6N"rrahman !an

Isw&r& JT) 01107+ Dain i#an yan

!ir%n!am $a!a 'ar"tan tawas ;5 4 905

s%'ama :1 m%nit sam$ai 0 *am) s%tia$ 91

ram !ain i#an m%ny%ra$ a'"mini "m

1)0


3/10

J"rna' K%s%hatan

K%'ainan F"nsi Hati Dan Gin*a' Ti#"s P"tih (Rattus norvegicus,L.) A#ibat S"$'%m%ntasi

Tawas Da'am Pa#an

a#'imatisasi !iana$ s%'%sai !an ti!a# a!a

h%wan "*i yan sa#it I mati) ma#a

$%mb%rian $a#an 6$%'%t7 !im"'ai !%nan

$%n/am$"ran tawas yan t%'ah !it%nt"#an

sam$ai batas wa#t" yan t%'ah !it%nt"#an

$"'a 6H"tahaian) S+) 9CC=7+

Pa!a awa' $%n%'itian) s"$'%m%ntasi

ta was !i'a#"#an !%nan !&sis 15 1)185

1) 9 5 1)05 1)85 !an 9 5) !%nan wa#t"

$a$aran - min" 6s%b%'"m ti#"s !ib%ri

$%r'a#"an7) ; min") < min" !an = min"+ S"$'%m%ntasi tawas !i'a#"#an !%nan

m%n/%# 6m%mas"##an 'ar"tan tawas

!%nan s&n!%7 'ar"tan tawas s%tia$ hari)

masin4masin 91 m'+ Hasi' $%m%ri#saan

'ab&rat&ri"m yan m%'i$"ti $%m%ri#saan

#%'ainan ,"nsi hati) #%'ainan ,"nsi in*a'

!an syst%m h%ma$&%ti# !ari ti#"s

$%r/&baan ti!a# m%n"n*"##an hasi' yan

b%rma#na+

Pa!a t"b"h) sanat m"n#in t%r*a!i

$%n"m$"#an i&n a'"min"m !ari tawas

a#ibat !ari s"$'ay '%wat ma#anan yan

ti!a# t%r#%n!a'i) ma#a !a'am $%r/&baan ini

!&sis $%r'a#"an 6s"$'%m%ntasi tawas $a!a

$a#an7 !inai##an m%n*a!i sat" '%3%' !i atas

!&sis $%r'a#"an t%rtini) yait" m"'ai !ari

05 :5 ;5 85 !an


4/10

J "rna' K%s%hatan

>&'+0) N&+ 0 D%s%mb%r011C 9;

K%'ainan F"nsi Hati Dan Gin*a' Ti#"s P"tih (Rattus norvegicus,L.) A#+ibatS"$'%m%ntasi

Tawas Da'am Pa#+an

A+0+ PEMERIKSAAN SGPT

M%t&!a $%m%ri#saan SGPT yan !i

"na#an a!a'ah U'tra >i&'%t T%st) yan$rinsi$nya a!a'ah ?

GPT

-#sa'&'"tarat + L 4 A'anin 4444L 4 G'"tamat + Pir"3at

M-H

Pir"3at +NADH + H 444444 L4 Ma'at +NAD

Pr&s%!"r $%m%ri#saan a!a'ah

s%baai b%ri#"t isi#an tab"n r%a#si!%nan sam$%' 6s%r"m !arah ti#"s7

s%banya# 01 ' !an !itambah r%a%n SGPT

s%banya# 9111 '+ .am$"r !an in#"basi#an

$a!a s"h" :2 . s%'ama 9 m%nit+ Ba/a

abs&rb%n s%t%'ah t%$at 9 m%nit) 0 m%nit) !an

: m%nit $a!a Pan*an G%'&mban :;1)

Fa#t&r 92;8!an $a!a Pr&ram K 01+

A+:+ PEMERIKSAAN T-TAL

BILLIRU BIN

P%m%ri#saan T&ta' Bi'ir"bin

m%n"na #an m%t&!a Gr&,, yan

m%r"$a#an m&!i,i#asi !ari m%t&!a

J%n!rasi#) yan $rinsi$nya a!a'ah bahwa

Bi'ir"bin b%r%a#si !%nan Dia(&h(%!

S"'$hanani'i/ A/i! m%mb%nt"# (at warna

A(& M%rah+

.aranya s%baai b%ri#"t ? !isia$#an 0

tab"n r%a#si) sat" tab"n "nt"# 'ar"tan

b'an#&) yait" 9111 ' R%a%n 9 !an 911 '

sam$%' 6s%r"m !arah ti#"s7+ S%!an tab"n

yan 'ain "nt"# sam$%') yait" !iisi 9111 '

!iin#"basi s%'ama 91 4 :1 m%nit $a!a

s"h" 08 .+ Ba/a $a!a Ph&t&m%t%r

!%nan Pan*an G%'&mban 8;


5/10

J"rna' K%s%hatan

K%'ainanF"nsiHatiDanGin*a'Ti#"sP"tih(Rattus norvegicus, L.) A#ibat S"$'%m%ntasi

Tawas Da'am Pa#an

/&'&rim%tri#+ Prinsi$ !asar !ari m%t&!a

t%rs%b"t a!a'ah bahwa "r%"m !ihi!r&'isis

!%nan a!anya air !an "r%as% m%mb%nt"#

am&nia !an .12 Pr&s%!"r $%m%ri#saan

#a!ar "r%"m !a'am !arah a!a'ah s%baai

b%ri#"t ? sia$#an : b"ah tab"n r%a#si+

Tab"n $%rtama !iisi !%nan 9111 '

r%a%n s%baai b'an#&+ Tab"n #% !"a

s%baai stan!ar!) !iisi 91 ' 'ar"tan

stan!ar! !an 9111 ' r%a%n 9+ tab"n #%

tia s%baai sam$%' !iisi 91 ' sam$%' !an9111 ' !an r%a%n 9) !i/am$"r !an

!iin#"basi $a!a s"h" :2. s%'ama : m%nit+

Masin4masin tab"n !itambah

!%nan 9111 ' r%a%n 0) !i/am$"r !an

!iin#"basi $a!a s"h" :2. sam$ai &'+0) N&+ 0 D%s%mb%r 011C 98


6/10

J"rna' K%s%hatan

>&'+0) N&+ 0 D%s%mb%r ;:1=

K%'ainan F"nsi Hati Dan Gin*a' Ti#"s P"tih (Rattus norvegicus, L.) A#ibat S"$'%m%ntasi

Tawas Da'am Pa#+an

Tab%' 9 ? Hasi' U*i P%nar"h S"$'%m%ntasi Tawas 15) 1)185) 1)95) 1)05) 1)85 !an 95A !a'am Pa#+an) t%rha!a$ 3ariab'% yan !i"*i

P%r'a#"an- !i ; m%

-'%h #ar%na s%m"a hasi' $%m%ri#saan m%nantisi$asi a!anya $%n"m$"#an tawas3ariab'% yan m%n"n*"##an ti!a# a!anya !a'am t"b"h) ma#a #&ns%ntrasi $%r'a#"an

$%nar"h s"$'%m%ntasi tawas $a!a $a#an !inai##an 9 '%3%' !i atasnya) yait"

#&ns%ntrasi 15) 1)185) 1)9 5) 1)05) 1)85 #&ns%ntrasi tawas yan !is"$'%m%ntasi#an!an 9 5 s%'ama $a$aran ; min") < #% !a'am $a#an m%n*a!i 15) 05) :5) ;5)min" !an = min") "nt"# 85 !an 1)18N&n sini

,i/ans

1)219

> 1)18N&n

sini,i/ans

;+ T&ta'

Pr&t%in

1)C=8

> 1)18N&n sini

,i/ans

1)89C

> 1)18N&n sini

,i/ans

1)C 1)18N&n

si(ni,i/ans

5. Ur%"m 1)::;

> 1)18N&n sini

,i/ans

1)8C=

> 1)18N&n sini

,i% ans1)2CC

> 1)18N&n

sini,i/ans

1)18N&n sini

,i/ans

1)= 1)18N&n sini

,i/ans

1)2C8

> 1)18N&n

sini,i/ans


7/10

J"rna' K%s%hatan


Tawas Da'am Pa#an

B+ PEMBAHASAN

Dari hasi' $%m%ri#saan 'ab&rat&ri"m

!an ana'isis statisti/) $%r'a#"ans"$'%m%ntasi tawas $a!a $a#an ti#"s

(Rattus norvegicus, L.) !%nan !&sis 9 5

1)85 1)05 1) I5 1)185) !an 15 6tan$a

s"$'%m%ntasi7 s%'ama wa#t" $a$aran ;

min") < min" !an = min") ti!a#

m%n"n*"##an hasi' yan b%rma#na 6n&n

sini,i/ans7+ Pa!a !&sis s"$'%m%ntasi tawas

$a!a $a#an &'+0)N&+ 0 D%s%mb%r 011C 92


8/10

J"rna' K%s%hatan

>&'+0)N&+0 D%s%mb%r ;:91

K%'ainan F"nsi Hati Dan Gin*a' Ti#"s P"tih (Rattus norvegicus,L.) A#ibat S"$'%m%ntasi

Tawas Da'am Pa#an

$arahnya #%r"sa#an yan t%r*a!i $a!a

*arinan hati !an in*a' ti#"s+

P%n"naan tawas "nt"#m%nin#at#an m"t" ma#anan) b%r!asar#an

KESIMPULAN DAN SARAN

A+ KESIMPULAN

Dari hasi' $%n%'itian !iatas) !a$at


9/10

J"rna' K%s%hatan

>&'+0)N&+0 D%s%mb%r ;:99

"*i &ran&'%$ti#) ti!a# m"n#in !a'am !isim$"'#an s%baai b%ri#"t) bahwa

#&ns%n trasi s%b%sar yan A'"mini "m !a'am tawas yan

!is"$'%m%ntasi#an $a!a ti#"s 6!a'am !is"$'%m%ntasi#an $a!a $a#an ti#"s)

$%r/&baan ini7) #ar%na *i#a #&ns%ntrasi

tawas yan !i/am$"r#an !a'am ma#anan

t%r'a'" banya# a#an m%nimb"'#an rasa

$ahit+

Ji#a ma#anan har"s !ir%n!am $a!a

'ar"tan tawas m"'ai !ari #&ns%ntrasi 9 5

sam$ai !%nan 905) s%$%rti yan !i'a#"#an

Haribi) R !an "srin 601187) #&ns%ntrasi

a'"mini"m yan t%ra#"m"'asi !a'am s%tia$

91 ram !ain i#an hanya 1) 0 4 1); $$m+

S%!an#an $a!a s"$'%m%ntasi tawas ini

s%na*a !imas"##an 'ar"tan tawas !%nan

s&n!% 'ans"n #% 'amb"n ti#"s !%nan

#&ns%ntrasi yan ti!a# biasa !i/am$"r#an

!a'am ma#anan+ Wa'a"$"n #&ns%ntrasi

'ar"tan tawas s%baai $%r%n!am ma#anan

6i#an7 m%n/a$ai 915) ma#a ti!a#

s%m"anya !ia#"m"'asi &'%h ma#anan

t%rs%b"t) #ar%na s%baian b%sar tawasb%ri#atan !%nan #&'&i! $a!a 'ar"tan

$%r%n!am+

A'"mini"m !a'arn 'in#"nan yan

asam b%rsi,at i&n) s%!an !a'am #&n!isi

$H n%tra' 6< 4 27 b%rsi,at s%baai '&am)

bah#an /%n!%r"n b%ri#atan !%nan bahan

&rani/ m%mb%nt"# #&'&i! 6 .h%"n) R.K)

et all, 01197+ Da'am t"b"h *asa! hi!"$

t%r*a!i #%s%imbanan asam !an basa)

s%hina $H t"b"h a!a'ah n%tra'+

!%nan #&ns%ntrasi !i atas 9 5 !%nan

wa#t" $a$aran ; min" s"!ahm%n"n*"##an a!anya $%r"sa#an *arinan

hati !an in*a'

K%r"sa#an *arinan hati !an in*a'

a#ibat s"$'%m%ntasi tawas $a!a $a#an m%n

*a!i '%bih $arah s%t%'ah wa#t" $a$aran

'%bih !ari = min"+ S%ma#in 'ama wa#t"

$a$aran !an s%ma#in tini !&sis

s"$'%m%ntasi tawas !a'am $a#an) a#an

s%ma#in $arah #%r"sa#an hati !an in*a'

ti#"s+

K&ns%ntrasi tawas yan !is"$'%m%n

tasi#an $a!a $a#an) s%ma#in tini m%m

S%/ara '&i#a) *i#a !i !a'am t"b"h

t%r!a$at a'"mi"m) a#an t%r*a!i i#atan

a'"mini"m !%nan bahan &rani/ t"b"h

m%mb%nt"# #&'&i!) s%'an*"tnya #&'&i!

!i#%'"ar#an !ari t"b"h '%wat ,a%s%s+D%nan

!%mi#ian ti!a# a#an t%r*a!i

a#"m"'asi a'" mini"m yan tini $a!a

&ran !a'am t"b"h yan m%mb%ri#an %,%#

tin#at #%r"sa#an *arinan hati !an in*a'

yan s%ma#in b%rat *"a+

B+SARAN

P%r'" !i'a#"#an ana'isis 6AAS I

Ray7 t%rha!a$ a'"mini"m $a!a &ran !an

$a!a ,a%s%s !an "rin%) "nt"# m%'a/a#


10/10

J "rna' K%s%hatan


Tawas Da'am Pa#an

t%r*a!inya a#"m"'asi a'"mini"m) ata"

'&am b%rat tsi# yan 'ain $a!a &ran)

,a%s%s !an "rin%+

DAFTARPUSTAKA

.h%"n) R+ .+K+ .han) M+ H+ M+ H&) .+S+ Lam)

.) W+ K+ an! La") E+ L+ K+) 0119+

H%a3y m%ta' $&is&nin /'ini/a'

sini,i/an/% an! 'ab&rat&ry

in3%stiati&n+ Asia $asi,i/ Ana'yt%

N&t%s+ B- In!is$%nsab'% t& H"man

H%a't+ >&' 2) N&+ I th 0119+ H&n

K&n+

Darmawati) S+ !an R+ Haribi) 0118+ Ana'isis $r&t%in

$i''i !ari Salmonella typhi is&'at RS

Karya!i S%maran !%nan

%'%#tr&,&r%sis S-S 4 PAGE J"ma'

Litban UNIMUS ISSN 9=0C4 ==1

>&'+ 0 N&+ : S%$t%mb%r 0118+

S%maran+

Haribi) R+ !an "srin) 0118+ K&ns%ntrasi

A'"mini"m $a!a '#an Asa$ yanDir%n!am !a'am Lar"tan Tawas+

P%n%'itian Dasar+ Dir*%n Di# Ti

D%$art%m%n P%n!i!i#an Nasi&na'+

Ja#arta+

H&,,bran!) A+>+) P%ttit) J+E+ an! M&ss P+A+H+

601187+ Ess%nsia' Ha%mat&'&y ; E! )

B'a/#w%'' S/i%n/%) Lt!+ -,&r!+

H"taha%an) S+)9CC=) P%nar"h F%nit&in t%rha!a$

Ka'si,i#asi S#%'%t&n) P%na$"ran

Art%ri !an K%/a/atan F%t"s M%n/it(*us musculus, L). T%sis Pr&ram

S"!i Bi&'&i+ Pr&ram Pas/a Sar*ana

Uni3%rsitas Ga!*ah Ma!a

B&ya#arta+

H"man G%s%''s/ha,t ,"r Bi&/h%mi/a "n!

Dian&sti/a mbH) 9CCC+ Ma 4 P'an/#

4 Rin 09 4 D 4 &'+0) N&+ 0 D%s%mb%r 011C 9C

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