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VARIASI SOMAKLONAL
Dr.Ir. Sukendah, MSc
Dasar Bioteknologi Tanaman
Variasi somaklonal
• Fenomena umum dalam regenerasi tanaman in vitro
• Keragaman somaklonal adalah keragaman genetik yang dihasilkan melalui kultur jaringan
• Keragaman genetik eksplan mutasi
• Keragaman genetik yang terjadi di dalam kultur jaringan
Fase tak berdiferensiasi yang relatif panjang
Pembentukan kalus
Massa sel parenkim yang terjadi karena proliferasi jaringan awal
•Somaclonal variation •Material utk mempelajari perkembangan •Dieksploitasi utk produksi bahan alam
Kumpulan sel amorphous dari sel-sel yang membelah diri terus menerus
Keuntungan utama
Plant improvement
Somaclonal variation dapat menciptakan tambahan variabilitas genetik
mahkota bunga
500 dan 1000 rad
gamma
In vitro
tunas
Pengakaran
Tanam di pot
Variasi Somaklonal Tujuan Khusus
1. Perubahan harus stabil 2. Perubahan harus merupakan sifat-sifat penting
seperti vigor, hasil, kemasakan, tipe tanaman, fertilitas, dan lain-lain
3. Variasi somaklonal yang menarik pada umumnya
meliputi sifat-sifat positif yang belum ada pada nomor-nomor galur yang dihasilkan oleh para pemulia tanaman
Komersial/ Crop Improvement Program
Terjadinya Variasi Somaklonal
1. Genetic (Heritable Variations)
• Pre-existing variations in the somatic cells of
explant (sudah ada variasi pada sel somatik
sebelumnya)
• Caused by mutations and other DNA changes
• Occur at high frequency
2. Epigenetic (Non-heritable Variations)
• Variations generated during tissue culture
(variasi yang diinduksi oleh kondisi kuljar)
• Caused by temporary phenotypic changes
• Occur at low frequency
Somaclonal Variation
Possible causes
the dedifferentiation – redifferentiation process
axillary shoot proliferation vs. organogenesis & embryogenesis
hypothesis of D'Amato
somaclonal variants are rare in micropropagated plants (when multiplication is by axillary branching of shoot tips/buds)
more common during shoot organogenesis & somatic embryogenesis (esp. w/a callus phase)
Somaclonal Variation
Possible causes
the "culture environment"
the hypothesis – that tissue culture is inherently stressful to cultured plant cells
environmental stress is known to cause:
DNA methylation – the methylation of cytosine is known to cause gene inactivation; this may occur during the redifferentiation process
gene amplification – can result in incr. gene expression
Somaclonal Variation Possible causes
the "culture environment"
environmental stress is known to cause:
transpositional changes
inadequate control of the cell cycle (errors in microtubule synthesis, spindle formation)
importance of PGRs
scant evidence of direct mutagenic action
more evidence for transient modifications of phenotype (e.g., dwarfing)
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