aed genetika mikroorganisme - mikrobiologifkunud.com · genom adalah keseluruhan informasi genetik...

dr. Agus Eka Darwinata, Ph.DGenetika Mikroorganisme

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The Central Dogma

Mutation

Gene dan Genome

Gene transfer

PCR

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Gene and Genome

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Genom adalah keseluruhan informasi genetik (gen) yang dimiliki suatu sel atau organisme.A

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Human GenomeA

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Bacterial GenomeA

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9181 bp

Viral GenomeA

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Genome-Gene AnalogyA

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The Central Dogma

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The Central Dogma of BiologyA

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DNA StructureA

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Replication

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Transcription

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Translation

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Translasi protein (cont.)A

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Mutation

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Mutation

! Changes in base sequece of DNA and inheritable"

! Can be:"! Harmful"! Lethal"! Helpful"! Silent

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Mutation

Point Mutation Frame Shift Mutation

missense

silent

nonsense

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Genetic Transfer

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Genetic Information Flow in BacteriaA

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Genetic Transfer

! Genetic transfer results in genetic variation"

! Genetic variation needed for evolution"

! Three ways:"! Transformation"! Conjugation"! Transduction

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Transformation! Genes transferred from one bacterium to another by virus.A

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Conjugation! Plasmid transferred from one bacterium to another via a pilus.A

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Conjugation cont…A

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PCR

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Polymerase Chain Reaction! PCR allows us to produce rapidly (amplify) a billion copies of DNA without needing a living cell. "

! These large quantities are then easily analyzed."

! PCR recipe:

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Polymerase Chain ReactionAmplification of nuc gene of S. aureus

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ResultA

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Thank you

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