vii

DAFTAR ISI

Halaman

HALAMAN JUDUL ...................................................................................... i

HALAMAN PENGESAHAN ........................................................................ ii

KATA PENGANTAR .................................................................................... iii

ABSTRAK ...................................................................................................... v

ABSTRACT .................................................................................................... vi

DAFTAR ISI .................................................................................................. vii

DAFTAR TABEL .......................................................................................... viii

DAFTAR GAMBAR ...................................................................................... ix

DAFTAR LAMPIRAN .................................................................................. x

BAB I PENDAHULUAN 1.1 Latar Belakang ......................................................................... 1

1.2 Rumusan Masalah .................................................................... 3

1.3 Tujuan Penelitian ..................................................................... 3

1.4 Manfaat Penelitian ................................................................... 3

1.5 Hipotesis ................................................................................... 4

BAB II TINJAUAN PUSTAKA 2.1 Luka.......................................................................................... 5

2.1.1 Pengertian Luka .............................................................. 5

2.1.2 Fase Penyembuhan Luka................................................. 5

2.1.3 Faktor Pendukung Proses Penyembuhan Luka ............... 7

2.1.4 Faktor Penghambat Proses Penyembuhan Luka ............. 8

2.2 Kolagen .................................................................................... 8

2.2.1 Pengertian Kolagen ......................................................... 8

2.2.2 Peran Kolagen Dalam Proses Penyembuhan Luka ......... 9

2.3 Anatomi Kulit .......................................................................... 10

2.4 Fisiologi Kulit .......................................................................... 11

2.5 Manggis (Garcinia Mangostana L.) ...................................... 12

2.5.1 Klasifikasi Manggis ......................................................... 12

2.5.2 Morfologi Manggis .......................................................... 13

2.5.3 Kandungan Kimia dan Manfaat Kulit Manggis .............. 13

2.6 Salep Sebagai Bahan Dasar Obat ............................................. 15

BAB III METODE PENELITIAN 3.1 Jenis dan Rancangan Penelitian ............................................... 16

3.2 Tempat dan Waktu Penelitian .................................................. 17

3.3 Subjek Penelitian ...................................................................... 17

3.4 Definisi Operasional................................................................. 17

3.5 Alat dan Bahan Penelitian ........................................................ 17

3.6 Prosedur Penelitian .................................................................. 19

3.6.1 Ekstraksi Kulit Manggis .................................................. 19

3.6.2 Pembuatan Salep Ekstrak Kulit Manggis ........................ 19

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3.6.3 Persiapan dan Pemeliharaan Hewan Coba ...................... 19

3.6.4 Perlakuan Terhadap Hewan Coba ................................... 20

3.6.5 Perawatan Luka ............................................................... 20

3.7 Parameter Yang Diamati .......................................................... 21

3.8 Analisa Statistik ....................................................................... 22

BAB IV HASIL DAN PEMBAHASAN

4.1 Hasil Ekstraksi Kulit Manggis ................................................. 23

4.2 Hasil Pembuatan Salep Ekstrak Kulit Manggis ....................... 23

4.3 Hasil Uji Potensi Salep Ekstrak Kulit Manggis

Terhadap Penyembuhan Luka Pada Mencit .............................. 23

4.3.1 Hasil Analisa Secara Maksroskopis ................................ 23

4.3.2 Hasil Analisa Kepadatan Jaringan Ikat Pada

Luka Mencit ..................................................................... 26

4.4 Pembahasan .............................................................................. 28

BAB V KESIMPULAN DAN SARAN

5.1 Kesimpulan .............................................................................. 32

5.2 Saran ......................................................................................... 32

DAFTAR PUSTAKA ..................................................................................... 33

LAMPIRAN .................................................................................................. 37

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DAFTAR TABEL

Halaman

Tabel 3.1 Rancangan Penelitian ...................................................................... 17

Tabel 3.2 Deskripsi Skor Jaringan Ikat atau Fibroblas ................................... 21

Tabel 4.1 Data Hari Penyembuhan Luka Pada Mencit ................................... 24

Tabel 4.2 Hasil Uji Levene Hari Penyembuhan Luka ..................................... 24

Tabel 4.3 Hasil Uji ANAVA Satu Arah Terhadap Penyembuhan Luka

Pada Mencit ...................................................................................... 25 25

Tabel 4.4 Hasil Uji Tuckey HSD Terhadap Penyembuhan Luka

Pada Mencit ..................................................................................... 25

Tabel 4.5 Perbandingan Skor Jaringan Ikat Pada Daerah Luka Mencit.......... 26

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DAFTAR GAMBAR

Halaman

Gambar 2.1 Penampang Melintang Kulit ...................................................... 10

Gambar 2.2 Manggis (Garcinia mangostana l.)............................................ 12

Gambar 4.1 Diagram Batang Kecepatan Hari Penyembuhan

Luka Mencit ............................................................................... 26

Gambar 4.2 Perbandingan ketebalan jaringan ikat kolagen pada

hari ke-3 ................................................................................... 27

Gambar 4.3 Perbandingan ketebalan jaringan ikat koloagen pada

hari ke-14 ................................................................................. 27

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DAFTAR LAMPIRAN

Halaman

Lampiran 1 Perancanaan Jadwal Pelaksanaan Penelitian ............................. 36

Lampiran 2 Alur Penelitian ........................................................................... 37

Lampiran 3 Skema Ekstraksi Kulit Manggis ................................................ 38

Lampiran 4 Hasil Pengolahan Data Penelitian .............................................. 39

Lampiran 5 Laporan Kerja Harian ................................................................ 41

Lampiran 6 Perbandingan Gambar Penyembuhan Luka Mencit .................. 42

Lampiran 7 Gambaran Jaringan Ikat Penyembuhan Luka Mencit ................ 44