pengaruh suhu dan lama pemanasan
TRANSCRIPT
8/18/2019 Pengaruh Suhu Dan Lama Pemanasan
http://slidepdf.com/reader/full/pengaruh-suhu-dan-lama-pemanasan 1/11
J u r n a l
S a i n s
da n
T e k n o h g i K i m i a
-
J i l i d
5 \ o. 2
O k to be r 2 0 1 4
I S S N 2 0 S 7 - 7 4 / 2
P e n g a r u h S u h u d a n L a m a P e m a n a s a n T e r h a d a p
A k t i v i t a s
A n t i o k s i d a n
d a n T o t a l A n t o s i a n i n M i n u m a n S a r i U b i J a l a r U n g u ( I p o m o e a B a t a ta s L . )
A r i n d a
F e b r i a n t i , G e b i
D w i y a n t i ,
W i w i S i s w a n i n g s i h
P r o g r a m
S t u d i
K i m i a
J u r u s a n P e n d i d i k a n K i m i a
F a k u l t a s P e n d i d i k a n M a t e m a t i k a d a n
I l m u
P c n g e t a h u a n
A l a m
U n i v e r s i t a s
P e n d i d i k a n I n d o n e s i a - B a n d u n g 4 0 1 5 4 , I n d o n e s i a
e m a i l :
g e b i . d w i y a n t i @ u p i . e d u
A B S T R A K
P e n e l i t i a n i n i b e r t u j u a n i m t u k m e n g e t a h u i k a n d u n g a n s c n y a w a m e t a b o l i t s e k u n d e r y a n g
t e r d a p a t d a l a m m i n u m a n s a r i
u b i
j a l a r u n g u y a n g b e r p e r a n s e b a g a i a n t i o k s i d a n ,
d a n
m e n g e t a h u i p e n g a r u h s u h u
d a n
l a m a p e m a n a s a n t e r h a d a p a k t i v i t a s a n t i o k s i d a n
d a n
t o t a l
a n t o s i a n i n
m i n u m a n s a r i u b i j a l a r u n g u ( I p o m o e a b a t a ta s L ) . U b i j a l a r u n g u d i o l a h m e n j a d i
m i n u m a n s a r i u b i j a l a r u n g u
m e l a l u i
p r o s e s p e m a n a s a n d e n g a n b e r b a g a i v a r i a s i s u h u , y a i t u
7 0 ° C , 8 0 ° C ,
d a n
90°C
d a n b e r b a g a i v a r i a s i l a m a p e m a n a s a n , y a i t u
5
m e n i t , 1 0 m e n i t , d a n
1 5
m e n i t . P e n e n t u a n k a n d u n g a n s e n y a w a m e t a b o l i t s e k u n d e r d i l a k u k a n
m e l a l u i
u j i f i t o k i m i a .
P e n e n t u a n
t o t a l
a n t o s i a n i n d i l a k u k a n d e n g a n m e n g g u n a k a n m e t o d e p e r b e d a a n p H . P e n e n t u a n
a k t i v i t a s
a n t i o k s i d a n d i l a k u k a n d e n g a n m e n g g u n a k a n m e t o d e
D P P H .
S e n y a w a m e t a b o l i t
s e k u n d e r y a n g t e r d a p a t d i d a l a m e k s t r a k u b i j a l a r u n g u d a n m i n u m a n s a r i u b i j a l a r u n g u y a i t u
a n t o s i a n i n ,
f l a v o n o i d ,
d a n
t e r p e n o i d . T o t a l a n t o s i a n i n m i n u m a n s a r i u b i j a l a r u n g u t e r j a d i
p e n u r u n a n d e n g a n r e n t a n g a n t a r a 2 1 5 , 0 8 m g / L
-
1 0 1 , 8 6 m g / L . A k t i v i t a s a n t i o k s i d a n m i n u m a n
s a r i
u b i j a l a r u n g u t e r j a d i p e n u r u n a n d e n g a n r e n t a n g a n t a r a 9 0 , 6 3
-
6 7 , 7 9 % .
A k t i v i t a s
a n t i o k s i d a n d a n
t o t a l
a n t o s i a n i n m i n u m a n s a r i u b i j a l a r u n g u s e m a k i n m e n u r u n s e i r i n g d e n g a n
m e n i n g k a t n y a
s u h u
d a n
l a m a p e m a n a s a n . K a r a k t e r i s t i k t e r b a i k t e r d a p a t
p a d a
m i n u m a n s a r i
u b i
j a l a r u n g u y a n g d i b u a t d e n g a n s u h u p e m a n a s a n
8 0 ° C .
P r o s e d u r y a n g m e n g h a s i l k a n
a k t i v i t a s a n t i o k s i d a n ,
t o t a l
a n t o s i a n i n ,
d a n
k e t a h a n a n t e r b a i k a d a t a h p r o s e d u r p e m b u a t a n
m i n u m a n s a r i u b i j a l a r u n g u p a d a s u h u p e m a n a s a n
80°C
s e l a m a
5
m e n i t .
K a t a k u n c i ;
a k t i v i t a s a n t i o k s i d a n , m i n u m a n s a r i u b i j a l a r u n g u , s u h u ,
t o t a l
a n t o s i a n i n
A B S T R A C T
T h e purpose o f
t h e
s tudy was to
d e t e r m i n e
the
c o n t e n t
o f secondary
m e t a b o l i t e s
in the
p u r p l e
sweet potato
j u i c e w h i c h has a r o l e as a n t i o x i d a n t , and d e t e r m i n e the e f f e c t of t e m p e r a t u r e
a n d d u r a t i o n
o f
h e a l i n g on a n t i o x i d a n t a c t i v it y an d t o ta l a n t h o c y a n i n of j u i c e p u r p l e
sweet
potato ( I p o m o e a b a t a ta s L). P u r p l e
sweet
potato was processed i n t o j u i c e p u r p l e
sweet
potato
t h r o u g h a p roces s o f h e a t i n g the t e m p e r a t u r e v a r i a t io n s , w h i c h is 7 0 ° C , S O ° C , an d 9 0 °C and
v a r i a ti o n s d u r a t i o n o f h e a t i n g , w h i c h is 5 m i n u t e s , 10 m i n u t e s , a nd 1 5 m i n u t e s. The secondary
m e t a b o l it e s w e r e d e t e r m i n e d
by
u s i n g
the
p h y t o c h e m i c a l test .
The
t o ta l a n t h o c y a n i n
was
d e t e r m i n e d
b y u s i n g
p H
d i f fe r e n t i a l m e t h o d .
The
a n t i o x i d a n t a c t i v i t y
was
d e t e r m i n e d
by
u s i n g
D P P H
m e t h o d . The
secondary
m e t a b o l it e s c o n t a i n e d in the e x t r a c t
o f
p u r p l e
sweet potato
a n d p u r p l e
sweet
potato
j u ic e a r e a n t h o c y a n i n s , f la v o n o i d s ,
and
t e r p e n o i d s . T o t a l a n t h o c y a n i n
o f
p u r p l e
sweet potato j u i c e was d e c l i n e in the r a n g e between 2 1 5 . O S mg/L • 1 0 1 . 8 6 mg/L. The
a n t i o x i d a n t a c t i v i t y o f p u r p l e sweet potato j u i c e was d e c l i n e in the r a n g e between 9 0 . 6 3 % -
6 7 . 7 9 % . A n t i o x i d a n t a c t i v i t y and t o ta l a n t h o c y a n i n p u r p l e sweet potato j u i c e decreases w i t h
i n c r e a s i n g t e m p e r a t u r e
an d
d u r a t i o n
o f
h e a t i n g .
The
best c h a r a c te r i s ti c f o u n d i n p u r p l e
sweet
8 5
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J u r m i l
S a in s d a n T e kn o lo g i K i m i a - J i l i d S
A o .
2 O k f o h e r 2 0 1 4
I S S N 2 0 S 7 - 7 4 I 2
potato
j u i c e w e r e p r e p a re d a t w a n n i n g t e m p e r a tu r e s o f 8 0 ° C . T h e c o n d i t io n s t h a t r e s u l te d i n
t h e
h i g h e s t
a n t i o x i d a n t
activity,
t o ta l a n t h o c y a n i n s , a n d
best
d u r a b i l it y a r e a t 8 0 ° C o f ' h e a t i n g
t e m p e r a t u r e f o r 5 m i n u t e s .
Keywords
: a n t i o x id a n t
activity,
j u i c e p u r p l e
sweet
p o t a t o ,
t e m p e r a t u r e , t o ta l a n t h o c y a n i n
P E N D A H L J L U A N
P e r g e s e r a n p o l a h i d u p m a n u s i a d a r i
p o l a h i d u p t r a d i s i o n a l m e n j a d i p o l a h i d u p
y a n g p r a k t i s d a n i n s t a n , m i s a l n y a p a d a
p c m i l i h a n m a k a n a n , m e m i l i k i d a m p a k
n e g a t i f
b a g i k e s e h a t a n . S c b a g i a n
b e s a r
m a s y a r a k a t l e b i h m e n y u k a i m e n g k o n s u m s i
m a k a n a n i n s t a n d a n
c e p a t
s a j i t a n p a
m e m p c r h a t i k a n k e c u k u p a n a s u p a n
v i t a m i n ,
m i n e r a l d a n k o m p o n e n - k o m p o n e n
f u n g s i o n a l
l a i n n y a d a r i m a k a n a n y a n g
m e r e k a k o n s u m s i . M a k a n a n i n s t a n d a n
c e p a t s a j i d e n g a n p e m a n a s a n t i n g g i d a n
p e m b a k a r a n m e r u p a k a n p i l i h a n d o m i n a n
y a n g d a p a t m e m i c u t e r b e n t u k n y a s e n y a w a
r a d i k a l
( P o u m o r a d e t a h , 2 0 0 6 ) . S e l a i n i t u ,
p e n i n g k a t a n p o l u t a n h a s i l p e m b a k a r a n
t i d a k
s e m p u r n a k e n d a r a a n b e r m o t o r d a n
i n d u s t r i s e p e r t i C O ( k a r b o n m o n o k s i d a ) ,
o k s i d a - o k s i d a n i t r o g e n , d a n h i d r o k a r b o n
m e r u p a k a n s e n y a w a - s e n y a w a y a n g r e n t a n
t e r o k s i d a s i m e n j a d i s e n y a w a r a d i k a l y a n g
d a p a t m e r u s a k t u b u h k i t a ( K u m a l a n i n g s i h ,
2 0 0 7 ) .
R a d i k a l b e b a s a d a l a h a t o m a t a u
m o l e k u l y a n g t i d a k s t a b i l d a n s a n g a t
r e a k t i f
k a r e n a m c n g a n d u n g s a t u a t a u l e b i h
e l e k t r o n
t i d a k
b c r p a s a n g a n p a d a
o r b i t a l
t c r l u a r n y a .
U n t u k m e n c a p a i k c s t a b i l a n
a t o m
a t a u m o l e k u l , r a d i k a l b e b a s a k a n
b e r e a k s i d e n g a n m o l e k u l d i s e k i t a r n y a
u n t u k m e m p e r o l e h p a s a n g a n e l e k t r o n . J i k a
r e a k s i i n i b e r l a n g s u n g t e r u s m e n e r u s d i
d a l a m s e l - s e l t u b u h d a n t i d a k d i h e n t i k a n
m a k a a k a n m e n i m b u l k a n b e r b a g a i
p e n y a k i t s e p e r t i k a n k c r , j a n t u n g , k a t a r a k ,
p e n u a a n d i n i , s e r t a p e n y a k i t d e g e n c r a t i f
l a i n n y a
( K u m a l a n i n g s i h , 2 0 0 7 ) . K e r u s a k a n
o k s i d a t i f a t a u k e r u s a k a n s e l a k i b a t r a d i k a l
b e b a s d a l a m t u b u h d a p a t d i a t a s i d e n g a n
s e n y a w a a n t i o k s i d a n y a n g m a m p u
m e n a n g k a l r a d i k a l b e b a s s e h i n g g a t i d a k
m e n i m b u l k a n
b e r b a g a i m a c a m p e n y a k i t
p a d a t u b u h k i t a . A n t i o k s i d a n b e r e a k s i
d e n g a n r a d i k a l b e b a s s e h i n g g a m e n g u r a n g i
k a p a s i t a s r a d i k a l b e b a s u n t u k
m e n i m b u l k a n
k e r u s a k a n ( C h a n g , e t a h ,
2 0 0 2 ) .
A n t i o k s i d a n m e r u p a k a n s u a t u
s e n y a w a y a n g d a p a t m e n g h a m b a t a t a u
m e n c e g a h p r o s e s o k s i d a s i s e n y a w a I a i n
y a n g d i a k i b a t k a n o l e h a d a n y a r a d i k a l
b e b a s ( W i n a r s i , 2 0 0 7 ) . B e r d a s a r k a n
s u m b e r n y a , a n t i o k s i d a n d i k e l o m p o k k a n
m e n j a d i a n t i o k s i d a n s i n t e t i k d a n
a n t i o k s i d a n a l a m i . P e n g g u n a a n a n t i o k s i d a n
s i n t e t i k s e m a k i n b e r k u r a n g k a r e n a d a p a t
m e n i m b u l k a n
e f e k n e g a t i f p a d a k e s e h a t a n
( k e r u s a k a n h a t i ) d a n d a p a t m e n i m b u l k a n
z a t k a r s i n o g e n s e h i n g g a p e n g g u n a a n n y a
p u n s e m a k i n t e r g a n t i k a n o l e h a n t i o k s i d a n
a l a m i
( P o u m o r a d , 2 0 0 6 ) . A n t i o k s i d a n
a l a m i b c r a s a l d a r i b u a h - b u a h a n , s a y u r a n ,
b i j i - b i j i a n d a n j u g a u m b i - u m b i a n . S a l a h
s a t u c o n t o h s u m b e r a n t i o k s i d a n a l a m i
a d a l a h u b i j a l a r u n g u .
U b i
j a l a r u n g u
( I p o m o e a b a t a t a s )
m e m i l i k i
k u l i t d a n d a g i n g
u m h i
y a n g
b e r w a r n a u n g u p e k a t . U b i j a l a r u n g u
m e n g a n d u n g p i g m e n w a r n a a n t o s i a n i n .
T o t a l k a n d u n g a n a n t o s i a n i n u b i j a l a r u n g u
a d a l a h 5 1 9 m g / 1 0 0 g b e r a t
b a s a h
( K a n o
e t
a h
y
2 0 0 5 ) . P i g m e n w a r n a u n g u ( a n t o s i a n i n )
p a d a u b i u n g u b e r m a n f a a t s e b a g a i
a n t i o k s i d a n k a r e n a
d a p a t
b e r e a k s i d e n g a n
r a d i k a l b e b a s d i d a l a m s e l - s e l t u b u h
s e h i n g g a d a p a t m e n g u r a n g i k a p a s i t a s
r a d i k a l b e b a s y a n g d a p a t m e n i m b u l k a n
k e r u s a k a n d i d a l a m t u b u h . M e n u r u t
K u m a l a n i n g s i h ( 2 0 0 7 ) , p i g m e n a n t o s i a n i n
y a n g t e r d a p a t d a l a m u b i j a l a r u n g u
m c m p u n y a i s t a b i l i t a s y a n g l e b i h t i n g g i b i l a
d i b a n d i n g k a n a n t o s i a n i n d a l a m s u m b e r
l a i n
s e p e r t i k u b i s m e r a h , e l d e r b e r r i e s ,
b l u e b e r r i e s d a n j a g u n g m e r a h . K c s t a b i l a n
p i g m e n a n t o s i a n i n d i p e n g a r u h i o l e h
8 6
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J u r n a t
S a i n s
d a n T e h i o l o g i K i m i a - J i t i d 5 N o . 2
O k t o b e r
2 0 1 4
{ S S N 2 0 S 7 - 7 4 / 2
c a h a y a ,
s u h u , d a n p H ( Y o s h i m o t o e l a l . ,
2 0 0 1 ) .
A n t o s i a n i n
m e r u p a k a n
s u b - t i p e
s e n y a w a o r g a n i k d a r i k c l u a r g a f l a v o n o i d ,
d a n m e r u p a k a n
a n g g o t a
k e l o m p o k
s e n y a w a y a n g l e b i h b e s a r
y a i t u p o l i f e n o l .
B e b c r a p a
s e n y a w a a n t o s i a n i n d i a n t a r a n y a
a d a l a h
p e l a r g o n i d i n , p e o n i d i n , s i a n i d i n ,
m a l v i d i n ,
p e t u n i d i n , d a n d e l f m i d i n . D u a
k o m p o n e n a n t o s i a n i n
y a i t u ,
s i a n i d i n 3 - 0 -
( 2 - 0 - ( 6 - 0 - ( E ) - c a f f c o l y - D -
G I
u
c o p y r a n o c y
1 - D - G I
u c o p y r a n o i
d
e ) - 5
- O -
D - G l u c o p y r a n i s i d e d a n p e o n i d i n 3 - 0 - ( 2 -
0 - ( 6 - 0 - ( E ) - c a f T e o t y - D - G l u c o p y r a n o c y l - D -
G l u c o p y r a n o i d e ) - 5 - 0 - D - G l u c o p y r a n i s i d e
y a n g
t e r d a p a t p a d a
u b i j a l a r u n g u
m e n u n j u k k a n a k t i v i t a s a n t i o k s i d a n ( K a n o
e t a i ,
2 0 0 5 ) .
B e r d a s a r k a n h a s i l
p e r c o b a a n
s e b e l u m n y a , a n t o s i a n i n d a r i u b i j a l a r u n g u
m e m p u n y a i f u n g s i f i s o l o g i s s e b a g a i
a k t i v i t a s
a n t i o k s i d a n d a n m e n g u r a n g i
k e r u s a k a n h a t i a k i b a t k a r b o n t e t r a k l o r i d a
( Y o s h i m o t o e t a l . ,
2 0 0 1 ) . D c l a p a n
k o m p o n e n u t a m a d a r i a n t o s i a n i n d a l a m u b i
j a l a r
u n g u m e n u n j u k k a n t i n g k a t a k t i v i t a s
a n t i o k s i d a n y a n g l e b i h t i n g g i d a r i
a s a m
a s k o r b a t
( H a r a d a
e t a l . ,
2 0 0 4 ) . P e n e l i t i a n
l a i n
m e n y e b u t k a n e k s t r a k u m b i u b i j a l a r
u n g u d a p a t d i g u n a k a n s e b a g a i a n t i o k s i d a n
p a d a
m e n c i t y a n g d i b e r i k a n
b e b a n
a k t i v i t a s
f i s i k m a k s i m a t ( J a w i
e t a l . ,
2 0 0 8 ) .
M c n g i n g a t b a n y a k m a n f a a t y a n g
t e r k a n d u n g d a l a m u b i j a l a r u n g u , m a k a
d i p e r l u k a n s u a t u i n o v a s i d a n k r c a s i
m e n g c n a i
p e n g o l a h a n
u b i j a l a r u n g u y a n g
m e m p u n y a i n i l a i a n t i o k s i d a n t i n g g i . S a l a h
s a t u n y a
y a i t u
i n c n g o l a h u b i j a l a r u n g u
m e n j a d i m i n u m a n s a r i u b i j a l a r u n g u .
P e n g o l a h a n u b i j a l a r u n g u m e n j a d i
m i n u m a n s a r i u b i j a l a r u n g u t i d a k
l e p a s
d a r i
p e r l a k u a n
p e m a n a s a n .
M e n u r u t H a y a t i
( 2 0 1 2 )
p r o s e s p e m a n a s a n d a p a t
m e m p e n g a r u h i k e s t a b i l a n a n t o s i a n i n y a n g
t e r d a p a t
p a d a u b i j a l a r u n g u . O l e h k a r e n a
i t u p e n e l i t i a n i n i b e r t u j u a n u n t u k
m e n g e t a h u i
c a r a p e n g o l a h a n
u b i j a l a r u n g u
y a n g
d a p a t
m c n g h a s i l k a n m i n u m a n s a r i u b i
j a l a r u n g u
d e n g a n
a k t i v i t a s a n t i o k s i d a n d a n
k a d a r a n t o s i a n i n t e r b a i k . S e l a i n i t u , u j i
f i t o k i m i a
p u n p c r l u d i l a k u k a n u n t u k
m e n g e t a h u i g o l o n g a n s e n y a w a m e t a b o l i t
s e k u n d e r
y a n g
t e r d a p a t
d i d a l a m m i n u m a n
s a r i u b i j a l a r u n g u y a n g
d a p a t
b e r f u n g s i
s e b a g a i
a n t i o k s i d a n .
M E T O D O L O G I P E N E L I T I A N
A l a t
P e r a l a t a n y a n g d i g u n a k a n p a d a
p e n e l i t i a n i n i m e l i p u t i a l a t - a t a t
g c l a s ,
s p a t u l a ,
p i s a u , p a r u t a n ,
n e r a c a
a n a l i t i k ,
b l e n d e r , p a n c i , k o m p o r ,
k e r t a s
s a r i n g ,
k a i n
s a r i n g , c o r o n g B u c h n e r , b o t o l v i a l ,
t e r m o m e t e r ,
h e a t e r , r o ta r y v a c u u m
e v a p o r a t o r ,
U V - V I S
M i n i
S h i m a d z u 1 2 4 0 .
B a h
a n
B a h a n u t a m a y a n g d i g u n a k a n
d a l a m p e n e l i t i a n i n i
a d a l a h
u b i j a l a r u n g u .
B a h a n l a i n n y a y a n g d i g u n a k a n p a d a p r o s e s
p e m b u a t a n
m i n u m a n s a r i u b i j a l a r u n g u
a d a l a h
g u l a
p a s i r
d a n a i r . B a h a n y a n g a k a n
d i g u n a k a n u n t u k p e n g u j i a n
a d a l a h
H C t 2
M , N a O H 2 M , H
2
S 0
4
p e k a t ,
C H 3 CO O H
H C I p e k a t , s e r b u k M g ,
F e C l
3
1 % i n d i k a t o r
p H ,
b u f T e r K C 1 p H 1 , 0 , b u f f e r N a - A s e t a t
p H
4 , 5 , m e t a n o l ,
D P P H ( 2 , 2 - D i p h e n y l - l -
p i c r y l h y d r a z y l ) , d a n a q u a d e s .
P r o s e d u r P e n e l i t i a n
D e t e r m i n a s i T u m b u h a n
U j i
d e t e r m i n a s i d i l a k u k a n d i
L a b o r a t o r i u m
S t r u k t u r T u m b u h a n ,
J u r u s a n
P e n d i d i k a n
B i o l o g i ,
F a k u l t a s P e n d i d i k a n
M a t e m a t i k a d a n l l m u P e n g e t a h u a n
A l a m ,
U n i v e r s i t a s P e n d i d i k a n I n d o n e s i a u n t u k
m e n g e t a h u i
k l a s i f i k a s i
b o t a n i t u m b u h a n
y a n g d i t e l i t i .
P e n y i a p a n S a m p e l U b i J a l a r U n g u
U b i
j a l a r u n g u d i s o r t a s i u n t u k
m c m i l i h
u b i j a l a r u n g u
d e n g a n
k u a l i t a s
y a n g b a i k k e m u d i a n
d i b u a n g b a g i a n
u b i
j a l a r u n g u y a n g t i d a k a k a n d i o l a h . S e t c l a h
i t u
u b i j a l a r u n g u d i c u c i
d e n g a n
a i r b c r s i h .
U n t u k p r o s e s
e k s t r a k s i , u b i j a l a r u n g u y a n g
t e l a h d i c u c i k e m u d i a n d i h a l u s k a n .
S e d a n g k a n u n t u k p r o s e s p e m b u a t a n
m i n u m a n
s a r i u b i j a l a r u n g u , u b i j a l a r u n g u
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J u r n a l
S a i n s
dan
T e b to io g i K i m i a
•
J i l i d
5
N o .
2
O k to be r 2 0 1 4 I S S N 2 0 8 7 - 7 4 1 2
y a n g t e l a h d i c u c i d e n g a n b e r s i h d i k u k u s
s e l a m a 1 5 m e n i t s a a t a i r m u l a i m e n d i d i h .
Ekstraksi Ubi Jal ar Ungu
S e b a n y a k 1 0 0 g r a m u b i j a l a r u n g u
y a n g t e l a h d i h a l u s k a n d i m a s e r a s i d e n g a n
2 0 0 m L m e t a n o l s e l a m a 1 x 2 4 j a m
d a n
d i m a s e r a s i j u g a d e n g a n 2 0 0 m L a q u a d e s
s e l a m a 1 x 2 4 j a m . E k s t r a k y a n g d i p c r o l e h
k e m u d i a n d i s a r i n g d a n d i p e k a t k a n d e n g a n
m e n g g u n a k a n
r o t a r y v a c u u m e v a p o r a t o r .
Pembuatan Minuman
Sari
Ubi Jalar
Ungu
U b i
j a l a r u n g u y a n g t e l a h d i k u k u s ,
d i k u p a s d a n d i p o t o n g k e c i l - k e c i l k e m u d i a n
d i t i m b a n g s e b a n y a k 1 0 0 g r a m d an
d i t a m b a h a q u a d e s 2 0 0 m l l a l u d i b l e n d e r
s a m p a i m e n j a d i b u b u r u b i j a l a r u n g u .
B u b u r u b i j a l a r u n g u d i s a r i n g d e n g a n
k a i n
s a r i n g d a n
f i l t r a t
h a s i l s a r i n g a n n y a
d i c a m p u r g u l a p a s i r . M c n u r u t S u h a r y o n o
( 2 0 0 8 ) , s u h u p e m a n a s a n p a d a p e m b u a t a n
s i r u p a d a l a h
85°C
d e n g a n l a m a p e m a n a s a n
1 0 m e n i t , s e h i n g g a
p a d a
p e n e l i t i a n i n i
d i l a k u k a n
v a r i a s i p e m a n a s a n
p a d a
s u h u
7 0 ° C , 8 0 ° C ,
d a n
9 °C
d a n v a r i a s i l a m a
w a k t u
p e m a n a s a n s e l a m a
5
1 0 ,
d a n 15
m e n i t . M i n u m a n s a r i u b i j a l a r u n g u y a n g
t e l a h d i p e r o l e h d i m a s u k k a n k e d a l a m l a b u
t a k a r 2 5 0 m L d a n d i t a n d a b a t a s k a n d e n g a n
a q u a d e s ,
l a l u d i m a s u k k a n k e d a l a m b o t o l
d a n d i p a s t e u r i s a s i
p a d a
s u h u
75°C
s e l a m a
1 0 m e n i t , k e m u d i a n m i n u m a n s a r i u b i j a l a r
u n g u d i d i n g i n k a n s e c a r a c e p a t .
U j i Fitokimia
U j i f i t o k i m i a
d i l a k u k a n
m e n g g u n a k a n m e t o d e m e n u r u t S a n g i
( 2 0 0 8 ) .
E k s t r a k d a n m i n u m a n s a r i u b i j a l a r
u n g u d i i d e n t i f i k a s i k o m p o n e n i t t o k i m i a n y a
d e n g a n m e t o d e p e r e a k s i w a r n a y a n g
b e r t u j u a n u n t u k m e n g e t a h u i g o l o n g a n
s e n y a w a m e t a b o l i t s e k u n d e r y a n g t e r d a p a t
d a l a m m a s i n g - m a s i n g s a m p e l y a n g
b e r f u n g s i
s e b a g a i
a n t i o k s i d a n .
U j i
f i t o k i m i a
y a n g d i l a k u k a n m e l i p u t i u j i
a n t o s i a n i n , u j i f l a v o n o i d , u j i t e r p e n o i d d a n
u j i
t a n i n .
U j i Total Antosianin
P e n g u k u r a n
t o t a l
k o n s c n t r a s i
a n t o s i a n i n d i l a k u k a n d e n g a n m e t o d e
p e r b e d a a n
p H m e n u r u t S t e e d ( 2 0 0 8 ) .
D i s i a p k a n
2
s a m p e l l a r u t a n , I a r u t a n
p e r t a m a a d a l a h l a r u t a n u n t u k
p H 1 0
m e n g g u n a k a n b u f f e r K C 1 - H C 1 ( 0 , 0 2 5 M )
d a n l a r u t a n k e d u a u n t u k p H 4 5
m e n g g u n a k a n b u f f e r N a - A s e t a t - H C l ( 0 , 4
M . D i a m b i l
m a s i n g - m a s i n g 1 m L e k s t r a k
u b i
j a l a r u n g u d a n 1 m L m i n u m a n s a r i u b i
j a l a r
u n g u d a r i b e r b a g a i s u h u p e m a n a s a n
d a n d i e n c e r k a n m e n g g u n a k a n I a r u t a n
b u f f e r m a s i n g - m a s i n g s a m p a i v o l u m e 1 0
m L ( f a k t o r p e n g e n c e r a n = 1 0 ) . D i l a k u k a n
p e n g u k u r a n a b s o r b a n s i
p a d a
p a n j a n g
g e l o m b a n g 5 3 0 ran d a n 7 0 0 n m u n t u k
s a m p e l h a s i l p e n g e n c e r a n , d a n u n t u k
m e n e n t u k a n
t o t a l
k o n s e n t r a s i n y a
d i g u n a k a n p e r s a m a a n b e r i k u t :
A X MW X DF X 10
T o t e d a n t o s t e m n ( m g / L ) = - — — - ^ — ^
Keterangan:
A : (Abs530 nm - Abs700 nm) pH 1 -
(Abs530 nm-Abs700 ran) pH 4,5
z :
Koefisicn ckstingsi molar (29.600 L
x mo "
1
x cm"
1
)
M W :
Bobot molekul
(449,2)
D F : Faklor pengenceran
1 : Tebal kuvet ( 1 cm)
U j i Aktivitas Antioksidan
P e n e n t u a n a k t i v i t a s a n t i o k s i d a n
d i l a k u k a n d e n g a n m e t o d e
D P P H
m e n u r u t
G a r c i a ( 2 0 1 2 ) y a n g d i m o d i f i k a s i . U n t u k
u j i
a k t i v i t a s a n t i o k s i d a n m i n u m a n s a r i u b i
j a l a r
u n g u , s e b a n y a k 0 5 m L s a m p e l
m i n u m a n
s a r i
u b i
j a l a r u n g u d i t a m b a h
d e n g a n 3 m L m e t a n o l d a n 0 , 3 m L D P P H
0 , 5 m M . C a m p u r a n t c r s c b u t d i i n k u b a s i
s e l a m a 1 0 0 m e n i t
p a d a
s u h u k a m a r
k e m u d i a n a b s o r b a n s i n y a d i u k u r d e n g a n
m e n g g u n a k a n s p e k t r o f o t o m c t e r
U V - V i s
p a d a
p a n j a n g g e l o m b a n g 5 1 7 n m . S e b a g a i
b l a n k o d i c a m p u r k a n 3 3 m L m e t a n o l
d e n g a n 0 , 5 m L s a m p e l . S e d a n g k a n u n t u k
k o n t r o l
d i b u a t d e n g a n m e n c a m p u r k a n 3 5
m L m e t a n o l d e n g a n 0 3 m L
D P P H
0 5
m M .
A k t i v i t a s
a n t i o k s i d a n
d a p a t
d i t e n t u k a n d e n g a n p e r s a m a a n b e r i k u t :
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J u r n a l S c r i m dan T e k n o h g i K i m i u -
M i l d
5 N o . 2 O k to b e r 2 0 1 4
I S S N 2 0 8 7 - 7 4 1 2
A itivitas Antioksidan =
1 0 0
-
{fibs
samp el -
b s bknb 1 1 0 0
s (central
U ji
Karakteristik Minuman Sari Ubi
Jalar
Ungu Selama Penyimpanan
M i n u m a n s a r i u b i j a l a r u n g u y a n g
d i p e r o l e h
d i u j i
k a r a k t e r i s t i k n y a s e l a m a
p e n y i m p a n a n
p a d a
s u h u k a m a r d e n g a n
c a r a
k o n v c n s i o n a l m e l a l u i p e n g a m a t a n
k o n d i s i
f i s i k d a n
p e n g u k u r a n
p H .
P e n g u j i a n d i l a k u k a n t e r h a d a p m i n u m a n
s a r i
u b i j a l a r u n g u y a n g d i b u a t p a d a s u h u
p e m a n a s a n
70°C, 80°C, 90°C
d a n l a m a
p e m a n a s a n 5 , 1 0 , 1 5 m e n i t .
S e b a n y a k 1 0 m l m i n u m a n s a r i u b i
j a l a r u n g u d i m a s u k k a n
k e
d a l a m m a s i n g -
m a s i n g t a b u n g r e a k s i d a n d i s i m p a n
p a d a
s u h u k a m a r . S a m p e l d i a m a t i p c r u b a h a n
k o n d i s i
f i s i k d a n j u g a d i l a k u k a n
p e n g u k u r a n p H s e t i a p h a r i s a m p a i t e r j a d i
p e r u b a h a n . U j i k a r a k t e r i s t i k m i n u m a n s a r i
u b i j a l a r u n g u s e l a m a p e n y i m p a n a n
d i l a k u k a n s e l a m a 5
h a r i .
H A S I L P E N E L I T I A N DAN
P E M B A H A S A N
Hasil
Determinasi Tumbuhan
H a s i l
d e t e r m i n a s i u b i j a l a r u n g u
y a n g d i p c r o l e h a d a l a h
s e b a g a i
b e r i k u t :
D i v i s i o : S p e r m a t o p h y t a
S u b d i v i s i o
: A n g i o s p e r m a e
K c l a s : D i c o t y l e d o n e a e
O r d o : C o n v o l v u l a l e s
F a m i l i a
: C o n v o l v u l a c e a e
G e n u s : I p o m o e a L .
S p e s i e s :
I p o m o e a
batatas ( L . ) L .
Hasil Ekstraks i Ubi Jal ar Ungu
E k s t r a k u b i j a l a r u n g u d i p e r o l e h
d e n g a n c a r a m a s e r a s i . P e m i l i h a n m e t o d e
m a s e r a s i i n i d i k a r e n a k a n m e t o d e m a s e r a s i
m c r u p a k a n s a l a h s a t u m e t o d e e k s t r a k s i
y a n g m u d a h k a r c n a h a n y a d i l a k u k a n
d e n g a n c a r a
m e r e n d a m s a m p e l d i d a l a m
p e l a r u t y a n g s e s u a i d a n j u g a a l a t - a l a t y a n g
d i g u n a k a n d a l a m m e t o d e i n i m c r u p a k a n
a l a t - a l a t y a n g
s e d e r h a n a ( H a y a t i ,
2 0 1 2 ) .
P r o s e s m a s e r a s i i n i t i d a k m c m e r l u k a n
p e m a n a s a n s e h i n g g a t i d a k a k a n m e r u s a k
s e n y a w a - s e n y a w a m c t a b o l i t s e k u n d e r y a n g
t e r k a n d u n g d a l a m u b i j a l a r u n g u .
E k s t r a k s i u b i j a l a r u n g u d i l a k u k a n
d e n g a n
m e n g g u n a k a n d u a b u a h p e l a r u t ,
y a i t u p e l a r u t a i r d a n m e t a n o l . A i r
d i g u n a k a n s e b a g a i p e l a r u t d a l a m p r o s e s
e k s t r a k s i d i k a r e n a k a n
a i r
m e r u p a k a n s a l a h
s a t u b a h a n y a n g d i g u n a k a n d a l a m
p e m b u a t a n p r o d u k m i n u m a n s a r i
u b i
j a l a r
u n g u . M e t a n o l d i g u n a k a n s e b a g a i p e l a r u t
k a r e n a m e t a n o l m e r u p a k a n p e l a r u t e f e k t i f
y a n g d a p a t m e l a r u t k a n h a m p i r s e l u r u h
s e n y a v v a m c t a b o l i t s e k u n d e r y a n g t e r d a p a t
d i d a l a m s a m p e l u b i j a l a r u n g u y a n g
d i e k s t r a k s i . M e t a n o l j u g a m e r u p a k a n
p e l a r u t y a n g m e m i l i k i t i t i k d i d i h y a n g
r e n d a h d a n m u d a h m e n g u a p s e h i n g g a
m u d a h d i p i s a h k a n d a r i e k s t r a k
u b i
j a l a r
u n g u .
E k s t r a k m e t a n o l y a n g d i p c r o l e h
d a r i h a s i l p e n y a r i n g a n k e m u d i a n d i u a p k a n
d e n g a n m e n g g u n a k a n r o ta r y v a c u u m
e v a p o r a t o r
d e n g a n
s u h u 46°C.
D a r i
h a s i l
p e n g u a p a n d i p e r o l e h e k s t r a k p e k a t u b i
j a l a r u n g u b c r w a r n a u n g u t u a d e n g a n
v o l u m e 2 7
m L
d a r i v o l u m e a w a l e k s t r a k
c a i r y a i t u 1 7 9 m L .
E k s t r a k
d e n g a n p e l a r u t a i r y a n g
d i p e r o l e h d a r i h a s i l p e n y a r i n g a n d i u a p k a n
m e n g g u n a k a n r o t a r y v a c u u m e v a p o r a t o r
d e n g a n
s u h u 68°C.
D a r i
h a s i l p e n g u a p a n
d i p e r o l e h e k s t r a k p e k a t
u b i
j a l a r u n g u
b c r w a r n a m c r a h a t i d e n g a n v o l u m e
1 2
m L
d a r i v o l u m e a w a l 1 7 6 m L .
Hasil
P r o d u k Minuman Sari Ubi J a l a r
Ungu
P e n g o l a h a n
u b i
j a l a r u n g u m e n j a d i
m i n u m a n
s a r i u b i j a l a r u n g u m e n g g u n a k a n
2 v a r i a b e l ,
y a i t u
v a r i a b e l s u h u
p e m a n a s a n
( 7 0 ° C , 8 0 ° C ,
d a n
9 0 ° C )
d a n v a r i a b e l l a m a
p e m a n a s a n ( 5 , 1 0 , da n 15 m e n i t ) . P a d a
a w a l
p r o s c d u r p e n g o l a h a n , s a m p e l u b i j a l a r
u n g u d i b l a n s i r t e r l e b i h d a h u t u . B l a n s i r
y a n g d i l a k u k a n p a d a s a m p e l u b i j a l a r u n g u
i n i m e n g g u n a k a n
u a p a i r
p a n a s m e l a l u i
c a r a p e n g u k u s a n s e l a m a 1 5 m e n i t . T u j u a n
u t a m a b l a n s i r a d a l a h u n t u k m e n g h e n t i k a n
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J u r n a l S a i n s dan T e k i w h g i K i m i a - J d id 5 N o . 2 O k i o b e r 2 0 1 4 J S S N 2 0 8 7 - 7 4 I 2
a k t i v i t a s e n z i m - e n z i m y a n g m e r u s a k m u t u
p r o d u k m i n u m a n s a r i u b i j a l a r u n g u y a n g
d i h a s i l k a n , s e h i n g g a d a p a t m e n j a g a w a r n a ,
r a s a , d a n
n u t r i s i
d a r i u b i j a l a r u n g u , s e r t a
m e n g u r a n g i j u m l a h m i k r o b a a w a l . D a r i
h a s i l p r c p a r a s i s a m p e l d i p e r o l e h f i l t r a t
( s a r i ) u b i j a l a r u n g u b e r w a r n a u n g u t u a ,
s e d a n g k a n r e s i d u b e r u p a p a d a t a n b e r w a r n a
u n g u m u d a . S a r i y a n g d i d a p a t k e m u d i a n
d i t a m b a h 1 0 g r a m g u l a d a n d i p a n a s k a n
b e r d a s a r k a n v a r i a b e l s u h u d a n l a m a
p e m a n a s a n y a n g t e l a h d i t e n t u k a n .
H a s i l y a n g d i p e r o l e h y a i t u
m i n u m a n
s a r i u b i j a l a r u n g u b e r w a r n a
u n g u t u a .
S e m a k i n t i n g g i s u h u
p e m a n a s a n
d a n s e m a k i n l a m a w a k t u p e m a n a s a n
m e n g h a s i l k a n m i n u m a n s a r i u b i j a l a r u n g u
d e n g a n w a r n a y a n g s e m a k i n p e k a t . P r o s e s
p e m a n a s a n a k a n m e n y e b a b k a n d e g r a d a s i
s e n y a w a a n t o s i a n i n m e n j a d i p r o d u k k e t o n
y a n g b e r w a r n a c o k l a t ( M a r k a k i s , 1 9 8 2 ) .
W a r n a
c o k l a t d a r i p r o d u k k e t o n i n i l a h
y a n g m e n y e b a b k a n w a r n a m i n u m a n s a r i
u b i
j a l a r u n g u m e n j a d i l e b i h p e k a t .
Hasil
Uji Fitokimia E k s t ra k Dan
Minuman S a r i Ubi J a la r Ungu
U j i
f i t o k i m i a a d a l a h u j i k u a l i t a t i f
d a r i s e n y a w a m e t a b o l i t s e k u n d e r y a n g
d i d a s a r k a n
a t a s
p e r u b a h a n w a r n a y a n g
t e r j a d i k c t i k a s a m p e l d i r e a k s i k a n d e n g a n
p e r e a k s i t e r t e n t u y a n g s e s u a i . U j i f i t o k i m i a
b e r t u j u a n u n t u k m e n e n t u k a n g o l o n g a n
s e n y a w a m e t a b o l i t s e k u n d e r a p a s a j a y a n g
t e r d a p a t d i d a l a m s a m p e l . H a s i l u j i
f i t o k i m i a e k s t r a k u b i j a l a r u n g u d a p a t
d i l i h a t p a d a
t a b e l 1 .
Tabel 1. Hasil Uji Fitokimia Ekstrak Ubi Jalar
Jenis Uji
Hasil
Jenis Uji
Ekstrak
Metanol
Ekstrak
A i r
Antosianin
(+)
(+)
Flavonoid
(+)
(+)
Terpenoid
(+) (+)
Tanin
(-) (-)
Sampel di
aeri tanda positif (+) j i k a
s a m p e l t e r i d e n t i f i k a s i m e n g a n d u n g j e n i s
g o l o n g a n s e n y a w a m e t a b o l i t s e k u n d e r
y a n g d i u j i . S e b a l i k n y a , s a m p e l d i b e r i t a n d a
n e g a t i f ( - ) j i k a s a m p e l t i d a k m e n g a n d u n g
j e n i s g o l o n g a n s e n y a w a m e t a b o l i t
s e k u n d e r y a n g
d i u j i .
H a s i l u j i f i t o k i m i a t e r h a d a p e k s t r a k
m e t a n o l d a n e k s t r a k a i r u b i j a l a r u n g u
m e n u n j u k k a n b a h w a a n t o s i a n i n , f l a v o n o i d ,
d a n t e r p e n o i d y a n g m e r u p a k a n s e n y a w a
m e t a b o l i t s e k u n d e r y a n g b e r p e r a n s e b a g a i
a n t i o k s i d a n d i d a l a m u b i j a l a r u n g u
t e r i d e n t i f i k a s i d a l a m k e d u a e k s t r a k .
S e d a n g k a n u n t u k u j i t a n i n , b a i k
p a d a
e k s t r a k m e t a n o l m a u p u n e k s t r a k a i r t i d a k
t e r b e n t u k w a r n a
b i r u
k e h i j a u a n , s e h i n g g a
d a p a t d i s i m p u l k a n b a h w a k e d u a e k s t r a k
t i d a k m e n g a n d u n g s e n y a w a t a n i n .
H a s i l
u j i f i t o k i m i a m i n u m a n s a r i
u b i
j a l a r u n g u
d a p a t
d i l i h a t p a d a t a b e l 2 .
Tabel 2. Hasil Uji Fitokimia Minuman Sari Ubi
Jalar Ungu
T
t
(nienil)
Jenis Uji Fitokimia
T
t
(nienil)
Antosi
anin
Flavo
noid
Terpen
oid
Tanin
7 °
C
5 (+)
(+)
<+)
(-)
7 °
C
10
< >
(+) (+)
(-)
7 °
C
15
(+)
(+) (+) (-)
8 0 °
C
5
(+)
(+)
(+) (-)
8 0 °
C
10
(+)
(+) (+)
(-)
8 0 °
C
1 5
(+) <+) (+>
(-)
9 °
C
5
(+) (+)
(+)
(-)
9 °
C
10
<+)
(+)
(+) <-)
9 °
C
1 5 (+)
(+)
w
(-)
B e r d a s a r k a n d a t a p a d a t a b e l
2
m i n u m a n s a r i u b i j a l a r u n g u y a n g d i b u a t
d e n g a n v a r i a s i s u h u p e m a n a s a n 7 0
C
C ,
8 0 ° C ,
d a n
9 °C
s e l a m a 5 , 1 0 , d a n 1 5 m e n i t
t e r i d e n t i f i k a s i m e n g a n d u n g s e n y a w a
m e t a b o l i t s e k u n d e r
y a i t u
a n t o s i a n i n ,
f l a v o n o i d ,
d a n t e r p e n o i d . S e d a n g k a n
s e l u r u h p r o d u k m i numan s a r i u b i j a l a r
u n g u t i d a k m e n g a n d u n g s e n y a w a t a n i n .
U j i
f i t o k i m i a t e r h a d a p e k s t r a k u b i
j a l a r u n g u , b a i k i t u e k s t r a k m e t a n o l d a n
e k s t r a k a i r , d a n j u g a m i n u m a n s a r i u b i j a l a r
u n g u m e n u n j u k k a n b a h w a e k s t r a k u b i j a l a r
u n g u d a n p r o d u k m i n u m a n s a r i u b i j a l a r
u n g u m e n g a n d u n g g o l o n g a n s e n y a w a
m e t a b o l i t s e k u n d e r y a n g d a p a t b e r t i n d a k
s e b a g a i a n t i o k s i d a n ,
y a i t u
a n t o s i a n i n ,
f l a v o n o i d d a n t e r p e n o i d . D e n g a n d e m i k i a n ,
p r o s e s
p e n g o l a h a n u b i j a l a r u n g u m e n j a d i
m i n u m a n s a r i u b i j a l a r u n g u t i d a k
9 0
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J u r n a l S a i n s dan T ek n ol o g i K i m i a - J i l i d 5 N o . 2 O k t o b e r 2 0 4
I S S N 2 0 8 7 - 7 4 1 2
m e n g u b a h g o l o n g a n s e n y a w a m e t a b o l i t
s e k u n d e r y a n g t e r k a n d u n g d i d a l a m u b i
j a l a r
u n g u .
Hasil
Uji
Total
Antosianin
Ekstrak
Dan
Minuman
Sari
Ubi
Jalar
Ungu
Menggunakan
Metode
Pcrbedaan Ph
U b i
j a l a r u n g u y a n g b e r w a r n a u n g u
m e n u n j u k a n
a d a n y a p e w a r n a a l a m i y a n g
t e r k a n d u n g d i d a l a m n y a y a i t u a n t o s i a n i n
y a n g m e r u p a k a n p i g m e n y a n g d a p a t l a r u t
d a l a m a i r d a n m e r u p a k a n s e n y a w a
m e t a b o l i t s e k u n d e r g o l o n g a n f l a v o n o i d
y a n g d a p a t b e r p e r a n s e b a g a i a n t i o k s i d a n d i
d a l a m u b i j a l a r u n g u . O l e h k a r e n a i t u p e r l u
d i k e t a h u i
k a d a r a n t o s i a n i n d i d a l a m
ub i
j a l a r
u n g u s e b e l u m d a n
s e s u d ah
d i o l a h
m e n j a d i m i n u m a n s a r i u b i j a l a r u n g u .
P e n g u k u r a n
t o t a l
a n t o s i a n i n e k s t r a k
d a n m i n u m a n s a r i u b i j a l a r u n g u d i l a k u k a n
d e n g a n m e n g g u n a k a n m e t o d e p e r b e d a a n
p H
i n i k a r e n a p a d a p H 1 a n t o s i a n i n
m e m b e n t u k s e n y a w a o x o n i u m ( k a t i o n
f l a v i l i u m )
y a n g b e r w a r n a m e r a h d a n
p a d a
p H 4 5
a n t o s i a n i n m e m b e n t u k
k a r b i n o l / h e m i k e t a l t a k b e r w a r n a ( H a y a t i ,
2 0 1 2 ) . K o n d i s i
i n i l a h
y a n g a k a n d i j a d i k a n
a c u a n u n t u k m e n e n t u k a n a b s o r b a n s i
d e n g a n
m e n g g u n a k a n s p e k t r o f o t o m e t c r
U v - V i s
d a r i m a s i n g - m a s i n g e k s t r a k d a n
m i n u m a n s a r i u b i j a l a r u n g u . P e r u b a h a n
w a r n a p a d a a n t o s i a n i n d a l a m t i n g k a t a n p H
t e r t e n t u d i s e b a b k a n s i f a t a n t o s i a n i n y a n g
m e m i l i k i
t i n g k a t k e s t a b i l a n y a n g b e r b e d a .
P a d a
p H 1 a n t o s i a n i n l e b i h s t a b i l da n
w a r n a l e b i h m e r a h d i b a n d i n g k a n p H 4 5
y a n g k u r a n g s t a b i l
d a n
b a m p i r t i d a k
b c r w a m a . S e l a i n f a k t o r p H a d a n y a
c a m p u r a n
d e n g a n
s e n y a w a
l a i n
( k o p i g m e n t a s i ) , k o n s e n t r a s i p i g m e n ,
j u m l a h g u g u s h i d r o k s i l d a n m e t o k s i j u g a
m e m p e n g a r u h i w a r n a d a r i a n t o s i a n i n
( S a t y a t a m a , 2 0 0 8 ) . S c r a p a n a n t o s i a n i n
y a n g d i l a r u t k a n d a l a m b u f f e r p a d a p H 1
d a n p H 4 5 d i u k u r p a d a p a n j a n g
g e l o m b a n g 5 3 0 n m y a n g m e r u p a k a n
p a n j a n g g e l o m b a n g m a k s i m a l d a r i
a n t o s i a n i n , s e d a n g k a n s e r a p a n p a d a
p a n j a n g g e l o m b a n g 7 0 0 n m d i l a k u k a n
s e b a g a i
f a k t o r k o r e k s i .
H a s i l
p c r h i t u n g a n
t o t a l
a n t o s i a n i n e k s t r a k u b i j a l a r u n g u
d a p a t d i l i h a t p a d a t a b e l 3 .
Tabel 3. Hasil Uji Total Antosianin Ekstrak Ubi
Jalar
Ungu
Sampel
Total Antosianin
(mg/L)
Ekstrak
metanol
361,69
mg/L
Ekstrak
air
253,15
mg/L
B e r d a s a r k a n t a b e l 3 d a p a t d i l i h a t
b a h w a
t o t a l
a n t o s i a n i n e k s t r a k m e t a n o l u b i
j a l a r
u n g u
d e n g a n
t o t a l
3 6 1 , 6 9 m g / L l e b i h
b e s a r
d i b a n d i n g k a n
d e n g a n t o t a l
a n t o s i a n i n
e k s t r a k a i r u b i j a l a r u n g u y a i t u s e b e s a r
2 5 3 , 1 5 m g / L . H a l i n i m e n u n j u k k a n b a h w a
p e l a r u t m e t a n o l l e b i h b a i k d i b a n d i n g k a n
d e n g a n
p e l a r u t
a i r
d a l a m h a l m e l a r u t k a n
a n t o s i a n i n .
H a s i l p c r h i t u n g a n
t o t a l
a n t o s i a n i n
m i n u m a n
s a r i u b i j a l a r u n g u d a p a t d i l i h a t
p a d a t a b e l 4 .
Tabel 4. Hasil Uji Total Antosianin Minuman Sari
W a k t u
Suhu Pemanasan
W a k t u
7 °C
8 °C
9 °C
5
menit
215,08
mg/L
181,01
mg/L
137,93
mg/L
10 menit
203,55
mg/L
175,00
mg/L
114,88
mg/L
15 menit
186,19
mg/L
148,28
mg/L
101,86
mg/L
P a d a
t a b e l
4 d a p a t
d i l i h a t b a h w a
s e m a k i n t i n g g i s u h u p e m a n a s a n dan
s e m a k i n l a m a p e m a n a s a n d a p a t
m e n u r u n k a n
t o t a l
a n t o s i a n i n d a l a m
m i n u m a n
s a r i u b i j a l a r u n g u . M i n u m a n s a r i
u b i j a l a r u n g u y a n g d i b u a t
p a d a
s u h u
p e m a n a s a n 70°C d a l a m w a k t u 5 m e n i t
m e m i l i k i
t o t a l
a n t o s i a n i n t e r b a i k
d i b a n d i n g k a n d e n g a n m i n u m a n s a r i u b i
j a l a r
u n g u l a i n n y a
y a i t u s e b e s a r
2 1 5 , 0 8
m g / L .
9 1
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J u r n a l S a i n s dan T ek n o lo gi K i m i a - J i l i d 5 N o . 2 O k t o be r 2 0 1 4
I S S N 2 0 8 7 - 7 4 1 2
D a r i
d a t a t o t a l
a n t o s i a n i n
d a p a t
d i k c t a h u i b a h w a a n t o s i a n i n b e r s i f a t t i d a k
s t a b i l d a n r c n t a n t e r h a d a p
p r o s e s
p e m a n a s a n ,
h a l i n i d i t u n j u k k a n d e n g a n
s e m a k i n m e n i n g k a t n y a s u h u
p e m a n a s a n
d a n l a m a
p e m a n a s a n ,
t o t a l
a n t o s i a n i n y a n g
t e r u k u r p u n s e m a k i n r e n d a h , d i k a r e n a k a n
t e r j a d i d e g r a d a s i a n t o s i a n i n . D e g r a d a s i
a n t o s i a n i n d a p a t m e n y e b a b k a n p e r u b a h a n
s t r u k t u r
a n t o s i a n i n m e n j a d i p r o d u k k e t o n .
T e r b e n t u k n y a p r o d u k k e t o n m e n y e b a b k a n
b e r k u r a n g n y a j u m l a h
g u g u s
h i d r o k s i l
f e n o l i k
a n t o s i a n i n y a n g b e r p e r a n
s e b a g a i
p e n d o n o r h i d r o g e n
k e p a d a
r a d i k a l b e b a s
s e h i n g g a m e n u r u n k a n k e m a m p u a n n y a
u n t u k
m e r e d a m r a d i k a l
b e b a s
( M a r k a k i s ,
1 9 8 2 ) .
Hasil Uji Aktivitas Antioksidan
Ekstrak
D a n Minuman Sar i Ubi J a l a r Ungu
Menggunakan Metode Dpph
A k t i v i t a s a n t i o k s i d a n e k s t r a k u b i
j a l a r
u n g u d a n m i n u m a n s a r i u b i j a l a r u n g u
d i t e n t u k a n m e l a l u i u j i a k t i v i t a s a n t i o k s i d a n
d e n g a n
m e t o d e
D P P H .
P r i n s i p d a r i m e t o d e
D P P H
a d a l a h
p e n a n g k a p a n
a t o m h i d r o g e n
o l e h r a d i k a l b e b a s D P P H d a r i zat
a n t i o k s i d a n . P e m i l i h a n m e t o d e D P P H i n i
d i k a r e n a k a n m e t o d e
D P P H
m e r u p a k a n
m e e
yto fe
M M
s e d e t a i d a n h a n y a
m e n g g u n a k a n s e d i k i t s a m p e l d e n g a n
w a k t u
p c n g e r j a a n y a n g s i n g k a t S e l a i n
d a r i p a d a i t u ,
D P P H
m e r u p a k a n r a d i k a l
y a n g s t a b i l s e h i n g g a p e n g g u n a a n n y a d a l a m
m e n e n t u k a n a k t i v i t a s a n t i o k s i d a n s a m p e l
j a u h
l e b i h m u d a h . P e n g u k u r a n a k t i v i t a s
a n t i o k s i d a n d i l a k u k a n
d e n g a n
m e n g g u n a k a n s p c k t r o f o t o m e t c r v i s i b l e
p a d a
p a n j a n g g e l o m b a n g 5 1 7 n m .
A n t i o k s i d a n
y a n g
t e r d a p a t
d a l a m
e k s t r a k d a n m i n u m a n s a r i u b i j a l a r u n g u
m e n g a k i b a t k a n t e r j a d i n y a p e r u b a h a n
w a r n a I a r u t a n D P P H . L a r u t a n
D P P H
b e r u b a h d a r i b e r w a r n a u n g u m e n j a d i
b e r w a r n a k u n i n g . H a l i n i t e r j a d i k a r e n a
r a d i k a l
D P P H
b e r e a k s i
d e n g a n
a t o m
h i d r o g e n y a n g d i d o n o r k a n o l e h s e n y a w a
a n t i o k s i d a n d a l a m e k s t r a k d a n m i n u m a n
s a r i u b i j a l a r u n g u , s e h i n g g a t e r b e n t u k
D P P H
t c r e d u k s i . S e m a k i n k u a t s u a t u
s e n y a w a a n t i o k s i d a n d a l a m m e n d o n o r k a n
a t o m h i d r o g e n n y a , m a k a a k a n s e m a k i n
t i n g g i
a k t i v i t a s a n t i o k s i d a n n y a .
H a s i l
p c r h i t u n g a n a k t i v i t a s a n t i o k s i d a n e k s t r a k
u b i j a l a r u n g u d a p a t d i l i h a t p a d a t a b e l 5 .
Tabel
5. Hasil Uji Total Antosianin
Ekstrak
Ubi
Jalar
Ungu
Sampel
Aktivitas Antioksidan
Sampel
(%)
Ekstrak metanol 97,92 %
Ekstrak
air
94,23
%
B e r d a s a r k a n t a b e l 5 d a p a t d i l i h a t
b a h w a a k t i v i t a s a n t i o k s i d a n e k s t r a k
m e t a n o l u b i j a l a r u n g u
d e n g a n p e r s e n t a s e
9 7 , 9 2 % l e b i h
b e s a r
d i b a n d i n g k a n
d e n g a n
a k t i v i t a s a n t i o k s i d a n e k s t r a k a i r u b i j a l a r
u n g u
y a i t u s e b e s a r
9 4 , 2 3 % . H a l i n i
m e n u n j u k k a n b a h w a m e t a n o l d a p a t
m e l a r u t k a n g o l o n g a n s e n y a w a m e t a b o l i t
s e k u n d e r y a n g
d a p a t
b e r p e r a n
s e b a g a i
a n t i o k s i d a n l e b i h b a i k d i b a n d i n g k a n
d e n g a n
p e l a r u t a i r .
H a s i l p c r h i t u n g a n a k t i v i t a s
a n t i o k s i d a n m i n u m a n s a r i u b i j a l a r u n g u
d a p a t
d i l i h a t
p a d a
t a b e l 6 .
Tabel
6. Hasil Uji Total Antosianin Minuman
Sari
U b i
Jalar
Uncu
Waktu
Suhu
Pemanasan
7 °C 8 °C
9 °C
5 menit
90,63
%
83,68
%
80,72
%
10 menit
87,65 % 82,41 % 77,54 %
15 menit
86,17%
81,35%
67,79 %
P a d a
t a b e l
6
d a p a t
d i l i h a t b a h w a
a k t i v i t a s a n t i o k s i d a n m c n u r u n s e i r i n g
d e n g a n
m e n i n g k a t n y a s u h u d a n l a m a
p e m a n a s a n .
M i n u m a n s a r i u b i j a l a r u n g u
y a n g d i b u a t
p a d a
s u h u
p e m a n a s a n 7 °C
9 2
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J u r n a l
S a i n s
dan T e k no lo gi K i m i a - J i l i d 5 N o . 2 O k i o be r 2 0 1 4
1 S S N 2 0 8 7 - 7 4 I 2
d a l a m w a k t u
5
m e n i t m e m i l i k i a k t i v i t a s
a n t i o k s i d a n t e r b a i k d i b a n d i n g k a n
d e n g a n
m i n u m a n s a r i u b i j a l a r u n g u l a i n n y a y a i t u
s e b e s a r 9 0 , 6 3 % .
D a r i d a t a a k t i v i t a s a n t i o k s i d a n
m e n u n j u k k a n b a h w a a n t i o k s i d a n r e n t a n
t e r h a d a p p r o s e s p e m a n a s a n .
R u s a k n y a
s e n y a w a a n t i o k s i d a n b e r p e n g a r u h
t e r h a d a p
a k t i v i t a s
a n t i o k s i d a n u b i j a l a r u n g u s e t e l a h
d i o l a h
m e n j a d i m i n u m a n s a r i
u b i
j a l a r
u n g u .
I n i
t e r l i h a t p a d a t e r j a d i n y a
p e n u r u n a n a k t i v i t a s a n t i o k s i d a n m i n u m a n
s a r i u b i j a l a r u n g u . S e m a k i n m e n i n g k a t n y a
s u h u
p e m a n a s a n d a n
l a m a
p e m a n a s a n
p e m b u a t a n m i n u m a n s a r i u b i j a l a r u n g u ,
a k t i v i t a s
a n t i o k s i d a n y a n g d i h a s i l k a n n y a
p u n s e m a k i n r e n d a h . S a l a h s a t u s e n y a w a
g o l o n g a n m e t a b o l i t s e k u n d e r d a l a m
u b i
j a l a r
u n g u y a n g d a p a t b e r t i n d a k
s e b a g a i
a n t i o k s i d a n a d a l a h a n t o s i a n i n . A n t o s i a n i n
s a n g a t r e n t a n
t e r h a d a p
p r o s e s p e m a n a s a n .
S u h u
p e n y i m p a n a n m a u p u n s u h u p r o s e s
p e n g o l a h a n y a n g t i n g g i a k a n m e n y a b a b k a n
d e g r a d a s i
s e n y a w a a n t o s i a n i n
( H a y a t i ,
2 0 1 2 ) .
A p a b i l a a n t o s i a n i n t e r d e g r a d a s i
m a k a
d a p a t
m e n y e b a b k a n p e r u b a h a n
s t r u k t u r
a n t o s i a n i n m e n j a d i p r o d u k k e t o n
y a n g
d a p a t
m e n u r u n k a n k e m a m p u a n n y a
u n t u k m e r e d a m r a d i k a l b e b a s s e h i n g g a
a K t i Y i t a s a n t i 9 k ?
i d a n n
y
a
p
u n
semakin
b e r k u r a n g .
Ha s i l
Pengujian
Karaktcr i s t ik
Minuman
Sari
Ubi
Jalar
Ungu Selama
Penyimpanan
P e n g u j i a n k a r a k t c r i s t i k m i n u m a n
s a r i
u b i
j a l a r u n g u s e l a m a p e n y i m p a n a n
p a d a s u h u k a m a r d i l a k u k a n m e l a l u i
p e n g a m a t a n k o n d i s i
f i s i k d a n
p e n g u k u r a n
p H p a d a
s e t i a p m i n u m a n s a r i
u b i
j a l a r
u n g u y a n g d i b u a t
d e n g a n
b e r b a g a i v a r i a s i
s u h u
p e m a n a s a n d a n
l a m a
p e m a n a s a n .
T u j u a n
d a r i p e n g u j i a n k a r a k t e r i s t i k
m i n u m a n
s a r i u b i j a l a r u n g u a d a l a h u n t u k
m e n c n t u k a n m i n u m a n s a r i u b i j a l a r u n g u
y a n g m e m p u n y a i k c t a h a n a n y a n g p a l i n g
b a i k . S a m p e l m i n u m a n s a r i u b i j a l a r u n g u
d i s i m p a n p a d a s u h u k a m a r dan
p e n g a m a t a n n y a d i l a k u k a n s a m p a i t e r j a d i
p e r u b a h a n k o n d i s i
f i s i k
d a n p e r u b a h a n p H .
U j i
k a r a k t e r i s t i k m i n u m a n s a r i
u b i
j a l a r
u n g u s e l a m a p e n y i m p a n a n d i l a k u k a n
s e l a m a
5
h a r i .
H a s i
D a r i h a s i l p e n g a m a t a n k o n d i s i
f i s i k
s a m p e l ( T a b e l
7 ) ,
d i k e t a h u i b a h w a
s u h u
p e m a n a s a n
d a p a t
m c n g h a m b a t
p e r t u m b u h a n m i k r o b a . H a l t e r s e b u t t e r l i h a t
p a d a s e m a k i n t i n g g i n y a s u h u p e m a n a s a n ,
m a k a p e r t u m b u h a n m i k r o b a p u n s e m a k i n
t e r h a m b a t s e h i n g g a k o n d i s i
f i s i k
m i n u m a n
s a r i u b i j a l a r u n g u p u n d a p a t l e b i h t e r j a g a .
B e r d a s a r k a n h a s i l p e n g u j i a n a k t i v i t a s
a n t i o k s i d a n d a n t o t a l a n t o s i a n i n m i n u m a n
s a r i u b i j a l a r u n g u y a n g m e m i l i k i a k t i v i t a s
a n t i o k s i d a n
d a n t o t a l
a n t o s i a n i n t e r t i n g g i
a d a l a h m i n u m a n s a r i u b i j a l a r u n g u y a n g
d i b u a t
p a d a
s u h u
p e m a n a s a n 7 0 ° C
s e l a m a
5 m e n i t
d e n g a n
a k t i v i t a s a n t i o k s i d a n
s e b e s a r 9 0 , 6 3 %
d a n t o t a l
a n t o s i a n i n
s e b e s a r 2 1 5 , 0 8 m g / L . N a m u n
p a d a
h a s i l
p e n g u j i a n k a r a k t c r i s t i k m i n u m a n s a r i u b i
j a l a r
u n g u s e l a m a p e n y i m p a n a n
m e n u n j u k a n b a h w a m i n u m a n s a r i u b i j a l a r
u n g u y a n g d i b u a t p a d a s u h u
p e m a n a s a n
7 0 ° C
s e l a m a
5
m e n i t m e n g a l a m i
p e r u b a h a n k o n d i s i
f i s i k
y a k n i
m e m u d a r n y a
w a m a ,
t i m b u l e n d a p a n d a n t i m b u l b u s a
d a l a m w a k t u 1
h a r i .
T a b e l
7 .
H a s i l
U j i K a r a k t e r i s t i k M i n u m a n S a r i U b i
J a l a r I
Suhu
dan L a m a Pemanasan
Hari
t
7 0 ° C
1 5
1 0 .
5
menit)
80
n
C
5
1 0 , 1 5
menit)
9 0 ° C
a
i o
i 5
menit)
0
Minuman sari
ubi jalar ungu
berwarna ungu
tua dan
memiliki pH 6
M inuinan
sari ubi jaiar
ungu
berwarna
ungu tua dan
memiliki pH
6
Minuman
sari ubi jalar
ungu
berwarna
ungu tua dan
memiliki pH
6
1
Mengalanii
pemudaran
wama, terdapat
endapan dun
busa. Memiliki
p H 5.
Mengalami
sedikit
pemudaran
wama dan
memiliki pH
6
Minuman
sari ubi jalar
ungu
berwarna
ungu tua dan
memiliki pH
6
2
Warna semakin
mcmudar, busa
semakin
banyak, tercium
bau masain.
Memiliki
pH 5.
Mengalami
pemudaran
wama,
terdapat
endapan dan
busa.
Memiliki pH
Mengalami
pemudaran
warna,
dan
inula)
tcrdapal
endapan.
Memiliki pH
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J u r n a l
S a i n s
dan
T ek no lo gi K i m i a
-
J i l i d
5
N o .
2
O k to be r 2 0 1 4
5.
5.
3
Warna
minuman
menjadi merah,
busa semakin
banyak.
Memiliki
pH 4,
Warna
minuman
menjadi
merah, busa
semakin
banyalt.
Memiliki
pH
5.
Wama
semakin
memudar,
dan
tenlapat
busa.
Memiliki
pH
5.
4
Wama
minuman
semakin merah,
tcrdapal lapisan
putih
dipennukaan.
Memiliki
pH 4.
Wama
minuman
merah,
busa
dan endapan
semakin
banyak.
Memiliki
pH
4.
Wama
minuman
menjadi
merah, busa
dan endapan
semakin
banyak.
Memiliki pH
4 .
B e r d a s a r k a n h a s i l p e n g u j i a n
k a r a k t e r i s t i k ,
m i n u m a n s a r i u b i j a l a r u n g u
y a n g m e m p u n y a i k e t a h a n a n t e r b a i k
t e r d a p a t p a d a
m i n u m a n s a r i u b i j a l a r u n g u
y a n g d i b u a t p a d a s u h u p e m a n a s a n 80°C.
D e n g a n d e m i k i a n , m i n u m a n s a r i u b i j a l a r
u n g u y a n g m e m i l i k i a k t i v i t a s a n t i o k s i d a n
d a n t o t a l a n t o s i a n i n y a n g c u k u p t i n g g i d a n
m e m i l i k i k e t a h a n a n y a n g c u k u p b a i k y a i t u
s e l a m a 2 h a r i , t e r d a p a t p a d a m i n u m a n s a r i
u b i j a l a r u n g u y a n g d i b u a t
p a d a
s u h u
p e m a n a s a n
8 °C
s e l a m a 5 m e n i t , d e n g a n
a k t i v i t a s
a n t i o k s i d a n s e b e s a r 8 3 , 6 8 % d a n
t o t a l
a n t o s i a n i n s e b e s a r 1 8 1 , 0 1 m g / L .
K E S I M P U L A N
B e r d a s a r k a n p e n e l i t i a n y a n g t e l a h
dilakukan,
d a p a t d i s i m p u l k a n b a h w a :
1 . K a n d u n g a n s e n y a w a m e t a b o l i t s e k u n d e r
y a n g
t e r d a p a t
d a l a m u b i j a l a r u n g u y a n g
b e r p e r a n
s e b a g a i
a n t i o k s i d a n a d a l a h
a n t o s i a n i n , f l a v o n o i d , t e r p e n o i d , dan
t a n i n .
2 .
A k t i v i t a s a n t i o k s i d a n d a n t o t a l
a n t o s i a n i n m i n u m a n s a r i u b i j a l a r u n g u
s e m a k i n m e n u r u n s e i r i n g
d e n g a n
m e n i n g k a t n y a s u h u
d a n
l a m a
p e m a n a s a n .
3 . P r o s e d u r p e m b u a t a n m i n u m a n s a r i u b i
j a l a r u n g u y a n g m c n g h a s i l k a n a k t i v i t a s
a n t i o k s i d a n , t o t a l a n t o s i a n i n , dan
k e t a h a n y a n g c u k u p b a i k a d a l a h
p r o s e d u r p e m b u a t a n m i n u m a n s a r i u b i
I S S N
2 0 8 7 - 7 4 1 2
j a l a r u n g u p a d a s u h u p e m a n a s a n 8 °C
s e l a m a
5
m e n i t .
D A F T A R P U S T A K A
C h a n g C ,
et
a / . ( 2 0 0 2 ) . E s t i m a t i o n o f
T o t a l
F l a v o n o i d
C o n t e n t i n P r o p o l i s b y
T w o
C o m p l e m e n t a r y C o l o r i m e t r i c
M e t h o d s . J . F o o d D r u g A n a t a y s i s ,
1 0 : 1 7 8 - 1 8 2 .
G a r c i a
E
et al. ( 2 0 1 2 ) . A n t i o x i d a n t
A c t i v i t y b y D P P H A s s a y o f
P o t e n t i a l S o l u t i o n s t o b e A p p l i e d
o n
B l e a c h e d T e e t h .
B r a z D e n t J
2 3 ( 1 ) :
2 2 - 2 7 .
H a r a d a ,
et a l
( 2 0 0 4 ) . A b s o r p t i o n
o f
A c y l a t e d
A n t h o c y a n i n s i n R a t s a n d
H u m a n s a f t e r I n g e s t i n g
a n
E x t r a c t
o f I p o m o e a b a t a t a s P u r p l e s w e e t
P o t a t o T u b e r .
B i o s c i . B i o e t e c h n o l . B i o c h e m ,
6 8 ( 7 ) ,
1 5 0 0 - 1 5 0 7 .
H a y a t i ,
E . , B u d i , U . , H e r m a w a n , R . ( 2 0 1 2 ) .
K o n s c n t r a s i t o t a l s e n y a w a
a n t o s i a n i n e k s t r a k k e l o p a k b u n g a
r o s e l l a ( H i b i s c u s s a b d a r i f f a L . ) :
P e n g a r u h t e m p e r a t u r d a n p H .
J u r n a l K i m i a ( J I N M a u l a n a M a l i k
I b r a h i m
M a k i n g
2:138 147.
J a w i , et al .
( 2 0 0 8 ) . U b i J a l a r U n g u
M e n u r u n k a n K a d a r M D A d a l a m
D a r a h d a n h a t i M c n c i t s e t e l a h
A k t i v i t a s
F i s i k
M a k s i m a l .
V e t e r i n e r ,
9 ( 2 ) , 6 5 - 7 2 .
K a n o , et al .
( 2 0 0 5 ) . A n l i o x i d a t i v e
A c t i v i t y o f A n t h o c y a n i n s
f r o m
P u r p l e S w e e t P o t a t o , I p o m o e a
b a t a t a s C u l t i v a r A y a m u r a s a k i .
Biosci. B i o e t e c h n o l . B i o c h e m . , 6 9
( 5 ) , 9 7 9 - 9 8 8 .
K u m a l a n i n g s i h . ( 2 0 0 7 ) .
A n t io k s id a n A l a m i
P e n a n g k a l R a d i k a l Bebas.
S u r a b a y a : T r u b u s A g r i s a r a n a .
M a r k a k i s , P . ( 1 9 8 2 ) . I n t r o d u c t i o n in
A n t h o c y a n i n in P r i n t s , V e g e ta b le ,
a n d G r a i n .
L o n d o n
:
C R C P r e s s .
P o u m o r a d , F . ,
et a l
( 2 0 0 6 ) . A n t i o x i d a n t
A c t i v i t y , P h e n o l a n d F l a v o n o i d
C o n t e n t s o f S o m e S e l e c t e d I r a n i a n
M e d i c i n a l P l a n t s . A f r i c a n J o u r n a l
9 4
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J u r n a l S a i n s J a n T ek no lo gi K i m i a - J i l i c l 5 N o . 2 O k i o b e r 2 0 1 4
I S S N 2 0 8 7 - 7 4 I 2
O f B i o t e c h n o l o g y ,
V o l ( 1 1 ) , 1 1 4 2 -
1 1 4 5 .
S a n g i , M . , d k k . ( 2 0 0 8 ) . A n a l i s i s
F i t o k i m i a T u m b u h a n O b a t d i
K a b u p a t e n M i n a h a s a U t a r a .
C h e m i s t r y
Progress.
1 ( I ) : 4 7 - 5 3 .
S a t y a t a m a , D . I . ( 2 0 0 8 ) . P e n g a r u h
K o p i g m e n t a s i T e r h a d a p S t a b i l i t a s
W a r n a A n t o s i a n i n B u a h D u w e t
( S y z y g i u m c u m i n i ) . Tests. B o g o r :
F a k u l t a s P e r t a n i a n . I n s t i t u t P e r t a n i a n
B o g o r .
S t e e d ,
L . E a n d V . D . T r u o n g . ( 2 0 0 8 ) .
A n t h o c y a n i n C o n t e n t , A n t i o x i d a n t
A c t i v i t y ,
a n d S e l e c t e d P h y s i c a l
P r o p e r t i e s o f F l o w a b l e P u r p l e
F l e s h e d S w e e t P o t a t o P u r e e s .
J o u r n a l of F o o d
Science, 7 3 ( 5 ) ,
2 1 5 - 2 2 1 .
S u h a r y o n o . ( 2 0 0 8 ) .
K h a s i a t
& M a n f a a t
J a h e M e r a h s i R i m p a n g A j a i b .
J a k a r t a :
A g r o M e d i a P u s t a k a .
W i n a r s i , H e r y . ( 2 0 0 7 ) .
A n t i o k s i d a n
A l a m i
d a n R a d i k a l
Bebas.
Y o g y a k a r t a :
K a n i s i u s .
Y o s h i m o t o ,
e t a l .
( 2 0 0 1 ) .
A n t i m u t a g e n i c i t y o f D e a c y l a t e d
A n t h o c y a n i n s i n P u r p l e - f l e s h e d
S w e e t p o t a t o .
Biosci.
B i o e t e c h n o l .
B i o c h e m . ,
6 5 ( 7 ) , 1 6 5 2 - 1 6 5 5 .