lampiran 1 gambar / foto sel mitosis pada jaringan karsinoma epidermoid...

76

LAMPIRAN 1

Gambar / foto sel mitosis pada jaringan karsinoma epidermoid (anak panah merah

dengan perbesaran 400x)

Kelompok kontrol Kelompok P1

Kelompok P2

Kelompok P3

77

LAMPIRAN 2

Analisa Statistik Perubahan Diameter Tumor

Case Summaries

Selisih diameter tumor

3 1.1000 .26458 1.0000 .90 1.403 .4333 .25166 .4000 .20 .703 .4000 .10000 .4000 .30 .503 -.2667 .20817 -.2000 -.50 -.10

12 .4167 .53739 .4000 -.50 1.40

KelompokKP1P2P3Total

N Mean Std. Deviation Median Minimum Maximum

Case Summaries

3 31.3000 2.4000

1.47986 1.34536.6000 2.0000

.30 1.303.00 3.90

3 3.5667 1.0000

.55076 .78102.3000 .6000

.20 .501.20 1.90

3 31.1000 1.5000

1.47986 1.57162.4000 .8000

.10 .402.80 3.30

3 3.9667 .7000

1.15902 .95394.4000 .2000

.20 .102.30 1.80

12 12.9833 1.4000

1.08362 1.22771.4000 1.0500

.10 .103.00 3.90

NMeanStd. DeviationMedianMinimumMaximumNMeanStd. DeviationMedianMinimumMaximumNMeanStd. DeviationMedianMinimumMaximumNMeanStd. DeviationMedianMinimumMaximumNMeanStd. DeviationMedianMinimumMaximum

KelompokK

P1

P2

P3

Total

Diametertumor pre test

Diametertumor post

test

78

NPar Tests Kruskal-Wallis Test

Tests of Normality

.314 3 . .893 3 .363

.219 3 . .987 3 .780

.175 3 . 1.000 3 1.000

.292 3 . .923 3 .463

KelompokKP1P2P3

Selisih diameter tumorStatistic df Sig. Statistic df Sig.

Kolmogorov-Smirnova Shapiro-Wilk

Lilliefors Significance Correctiona.

Test of Homogeneity of Variances


1.129 3 8 .394

LeveneStatistic df1 df2 Sig.

79

Mann Whitney Test

Test Statisticsb

.0006.000

-1.964 .049

.100a

Mann-Whitney UWilcoxon WZAsymp. Sig. (2-tailed) Exact Sig. [2*(1-tailedSig.)]

Tumordiameterdiference

Not corrected for ties.a.

Grouping Variable: Groupb.

Test Statisticsb

.0006.000

-1.964 .049

.100a





80

Test Statisticsb

.0006.000

-1.964 .049

.100a





81

Test Statisticsb

.0006.000

-1.964 .049

.100a





Test Statisticsb

.0006.000

-1.964 .049

.100a





82

LAMPIRAN 3

Analisa Statistik Indek Mitosis

NPar Tests

Kruskal-Wallis Test

Case Summaries

Index mitosis

3 3.2000 .34641 3.4000 2.80 3.403 1.1333 .11547 1.2000 1.00 1.203 1.0667 .11547 1.0000 1.00 1.203 .6667 .11547 .6000 .60 .80

12 1.5167 1.04606 1.1000 .60 3.40


N Mean Std. Deviation Median Minimum Maximum

Tests of Normality

.385 3 . .750 3 .000

.385 3 . .750 3 .000

.385 3 . .750 3 .000

.385 3 . .750 3 .000

KelompokKP1P2P3

Index mitosisStatistic df Sig. Statistic df Sig.

Kolmogorov-Smirnova Shapiro-Wilk


Test of Homogeneity of Variances

Index mitosis

5.333 3 8 .026

LeveneStatistic df1 df2 Sig.

Ranks

3 11.003 7.003 6.003 2.00

12


Index mitosisN Mean Rank

83

NPar Tests

Mann-Whitney Test

NPar Tests

Mann-Whitney Test

Test Statisticsa,b

9.8043

.020

Chi-SquaredfAsymp. Sig.

Index mitosis

Kruskal Wallis Testa.

Grouping Variable: Kelompokb.

Ranks

3 5.00 15.003 2.00 6.006

KelompokKP1Total

Index mitosisN Mean Rank Sum of Ranks

Ranks

3 5.00 15.003 2.00 6.006

KelompokKP2Total


Test Statisticsb

.0006.000

-2.023.043

.100a

Mann-Whitney UWilcoxon WZAsymp. Sig. (2-tailed)Exact Sig. [2*(1-tailedSig.)]

Index mitosis



84

NPar Tests

Mann-Whitney Test

NPar Tests

Mann-Whitney Test

Ranks

3 5.00 15.003 2.00 6.006

KelompokKP3Total


Test Statisticsb

.0006.000

-2.023.043

.100a


Index mitosis



Ranks

3 4.00 12.003 3.00 9.006

KelompokP1P2Total


85

NPar Tests

Mann-Whitney Test

Test Statisticsb

3.0009.000-.745.456

.700a


Index mitosis



Ranks

3 5.00 15.003 2.00 6.006

KelompokP1P3Total


Test Statisticsb

.0006.000

-2.023.043

.100a


Index mitosis



86

NPar Tests

Mann-Whitney Test

Ranks

3 5.00 15.003 2.00 6.006

KelompokP2P3Total


Test Statisticsb

.0006.000

-2.023.043

.100a


Index mitosis



87

LAMPIRAN 4

Uji Korelasi Selisih Diameter Tumor dan Indek Mitosis

Explore

Nonparametric Correlations

Case Summaries

12 12.4167 1.5167

.53739 1.04606.4000 1.1000

-.50 .601.40 3.40

NMeanStd. DeviationMedianMinimumMaximum

Selisihdiameter

tumor Index mitosis

Tests of Normality

.105 12 .200* .988 12 .999

.369 12 .000 .742 12 .002Selisih diameter tumorIndex mitosis

Statistic df Sig. Statistic df Sig.Kolmogorov-Smirnova Shapiro-Wilk

This is a lower bound of the true significance.*.


Correlations

1.000 .813**. .001

12 12.813** 1.000.001 .

12 12

Correlation CoefficientSig. (2-tailed)NCorrelation CoefficientSig. (2-tailed)N


Index mitosis

Spearman's rho

Selisihdiameter

tumor Index mitosis

Correlation is significant at the 0.01 level (2-tailed).**.

88

LAMPIRAN 5

Patologi Anatomi Sampel, Ukuran Diameter Tumor Pre dan Post Perlakuan, Indek Mitosis Post Perlakuan

SCORING INDEX MITOSIS DALAM 5 LAPANG PANDANG BESAR

HASIL PATOLOGI ANATOMI

KELOMPOK I II III IV V Mean Kontrol

A 4 3 3 5 2 3.4 SQUAMOUS CELL CA WELL DIFFERENTIATED

B 2 3 4 3 2 2.8 SQUAMOUS CELL CA Insitu C 4 3 2 3 5 3.4 SQUAMOUS CELL CA Insitu

mean 3.2 P1

A 1 2 1 1 1 1.2 KERATOACANTOMA DENGAN SQUAMOUS CELL CA MIKROINVASIF

B 0 1 2 1 1 1 PAPILOMA CELL SQUAMOUS DENGAN FOKUS CA

C 3 1 1 1 0 1.2 SQUAMOUS CELL CA Insitu Mean 3.4

P2

A 1 1 1 0 2 1 SQUAMOUS CELL CA WELL DIFF DENGAN NEKROSIS LUAS

B 2 2 0 1 1 1.2 SQUAMOUS CELL CA WELL DIFFERENTIATED

C 1 0 2 2 0 1 SQUAMOUS CELL CA WELL DIFFERENTIATED

mean 1.06 P3

A 0 1 1 2 0 0.8 SQUAMOUS CELL CA WELL DIFFERENTIATED

B 1 0 0 1 1 0.6 SQUAMOUS CELL CA Insitu

C 1 1 0 1 0 0.6 SQUAMOUS CELL CA INSITU + MIKROINVASIF

mean

0.6

89

Data Ukuran Diameter Tumor Pre dan Post Perlakuan

Kelompok Diameter

tumor pre test (cm)

Diameter tumor post

test (cm)

Selisih diameter tumor (cm)

Kontrol A 3 3,9 0,9 B 0,6 2 1,4 C 0,3 1,3 1

Mean 1,1 P1 A 1,2 1,9 0,7 B 0,2 0,6 0,4 C 0,3 0,5 0,2

Mean 0,43 P2 A 2,8 3,3 0,5 B 0,1 0,4 0,3 C 0,4 0,8 0,4

Mean 0,4 P3 A 2,3 1,8 -0,5 B 0,4 0,2 -0,2 C 0,2 0,1 -0,1

Mean -0,26

Keterangan :

K : Kontrol

P1 : Phaleria macrocarpa 0,0715 mg (0,36 ml/hari).

P2 : sitostatika Paclitaxel 175 mg/m2 dan cisplatin 50 mg/m2

P3 : sitostatika Paclitaxel 175 mg/m2 dan cisplatin 50 mg/m2 dan dikombinasikan dengan Phaleria macrocarpa 0,0715 mg (0,36 ml/hari)

A : tanda asam pikrat pada mencit di kepala

B : tanda asam pikrat pada mencit di kaki depan

C : tanda asam pikrat pada mencit di ekor

90

LAMPIRAN 6

Foto Mencit yang Diinduksi Tumor, Perlakuan, dan Pengukuran Tumor

Gambar. Mencit yang diinduksi karsinoma epidermoid

Gambar. Pemberian Phaleria macrocarpa via sonde

Gambar. Penyuntikan sitostatika

Gambar. Pengukuran diameter tumor menggunakan caliper

91

LAMPIRAN 7

Foto Pemeliharaan Mencit

92

LAMPIRAN 8

Foto Pengolahan Ekstrak Phaleria macrocarpa

93

LAMPIRAN 9

94

LAMPIRAN 10

lampiran 1 gambar / foto sel mitosis pada jaringan karsinoma epidermoid...

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