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Lampiran 1

METODE BAKU HISTOLOGIS PEMERIKSAAN JARINGAN

(TjartaAchmad, 1992)

A. Cara pengambilan jaringan dan fiksasi

1) Mengambil jaringan sesegera mungkin setelah tikus Wistar dewasa

diterminasi dengan cara pemberian Choloroform dosis sangat

tinggi.

2) Kemudian memasukkan ke dalam larutan fiksasi dan dekalsifier

dengan urutan sebagai berikut :

a) Fiksasi dalam larutan formalin 10%

b) Dekalsifikasi menggunakan TBD-1 rapid declacifier selama 24

jam untuk melunakkan tulang sebelum di masukkan dalam blok

parafin.

c) Xylol paraffin 1:1 selama 20 menit/24 jam dengan dipanaskan

dalam oven 600C.

d) Embedding dan blocking : paraffin 1, 2, 3 selama 20 menit, lalu

jaringan dicetak blok paraffin kemudian didinginkan, sehingga

cetakan dapat dibuka.

e) Trimming : memotong balok-balok paraffin sehingga jaringan

mudah dipotong dengan mikrotom.

B. Cara pemotongan blok (sectioning)

1) Menyiapkan kaca objek bersih.

2) Kaca objek diberi albumin ditengahnya dan direkatkan.

3) Blok yang sudah disiapkan dipotong dengan ketebalan 5

mikron, lalu dimasukkan dalam air panas kurang lebih 600C.

Setelah jaringan mengembang jaringan diambil dengan kaca

objek yang sudah diberi albumin.

4) Kemudian dikeringkan.

5) Paraffin yang ada pada kaca objek atau jaringan dihilangkan

dengan dipanaskan dalam oven 600C atau dengan tungku.

B. Pewarnaan HE

Slide jaringan dimasukkan dalam :

1) Xylol 1, 2, 3 masing-masing 10 menit.

2) Rehidrasi dengan alkohol xylol selama 5 menit.

3) Bilas alkohol 30-96% masing-masing kurang lebih 30 menit.

4) Bilas aquades 1 kali kurang lebih 10 menit.

5) Rendam dalam hematoksilin kurang lebih 10 menit.

6) Bilas dengan air mengalir sampai bersih.

7) Bilas aquades, lalu acid alcohol (alkohol+NaCl 0,9%).

8) Bilas alkohol 50-96%.

9) Eosin kurang lebih 2-5%.

10) Bilas alkohol 96% sebanyak 2 kali.

11) Bilas alkohol xylol.

12) Keringkan dengan kertas saring langsung dibersihkan kotoran-

kotoran yang ada di sekitar jaringan.

13) Xylol 1 (5 menit), xylol 2 (5 menit) tetesi asam canada,

langsung ditutup kaca penutup.

14) Preparat sudah siap untuk diamati di atas mikroskop.

Lampiran 2

Lampiran 3

Lampiran 4. Hasil Pembacaan Slide Penelitian

Tikus

Lapangan Pandang

I II III

Kontrol 1 5 5 4

Kontrol 2 5 4 5

Kontrol 3 5 3 4

Kontrol 4 4 4 5

Kontrol 5 5 5 4

Kontrol 6 5 4 4

Kontrol 7 5 3 4

Perlakuan 1 3 3 4

Perlakuan 2 3 4 4

Perlakuan 3 4 4 3

Perlakuan 4 4 4 3

Perlakuan 5 4 3 5

Perlakuan 6 3 3 4

Perlakuan 7 4 4 4

Lampiran 5. Analisis data menggunakan program SPSS

Descriptives

Statistic Std. Error

Kontrol Mean 4.3814 .11403

95% Confidence Interval for Mean Lower Bound 4.1024

Upper Bound 4.6604

5% Trimmed Mean 4.3866

Median 4.3300

Variance .091

Std. Deviation .30169

Minimum 4.00

Maximum 4.67

Range .67

Interquartile Range .67

Skewness -.333 .794

Kurtosis -1.842 1.587

Perlakuan Mean 3.6671 .10339

95% Confidence Interval for Mean Lower Bound 3.4142

Upper Bound 3.9201

5% Trimmed Mean 3.6674

Median 3.6700

Variance .075

Std. Deviation .27354

Minimum 3.33

Maximum 4.00

Range .67

Interquartile Range .67

Skewness -.033 .794

Kurtosis -1.200 1.587

Tests of Normality

Kolmogorov-Smirnova

Shapiro-Wilk

Statistic df Sig. Statistic df Sig.

Kontrol .259 7 .170 .818 7 .062

Perlakuan .218 7 .200*

.858 7 .144

*. This is a lower bound of the true significance.

a. Lilliefors Significance Correction

Group Statistics

Kelompok N Mean Std. Deviation Std. Error Mean

Derajat Penyembuhan Fraktur 1.00 7 3.6671 .27354 .10339

2.00 7 4.3814 .30169 .11403

Lampiran 6. Dokumentasi Penelitian dan Gambaran Mikroskopis Kalus

Pemberian label pada tikus

Anestesi dengan ketamine IM

Frakturisasi tertutup

Injeksi ketorolac intraperitoneal hari pertama

Blok Paraffin

Pengecatan HE

Skor Allen 3

Skor Allen 4

Skor Allen 5

Skor Allen 5